Chemical and enzymatic characterization of recombinant rabbit muscle pyruvate kinase

Biological Chemistry

Volumn 394, Issue 5, 2013, Pages 695-701

  Boehme, Christian ^a,b   Bieber, Frank ^b   Linnemann, Julia ^b   Breitling, Reinhard ^c   Lorkowski, Stefan ^a   Reissmann, Siegmund ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b LAROVA GmbH   (Germany)

^c JenaBios GmbH   (Germany)

Author keywords

Comparison to native enzyme; Enzymatic synthesis of thymidine triphosphate; Influence of metal ions; Kinetic parameters; Purification; Recombinant expression

Indexed keywords

ADENOSINE DIPHOSPHATE; DEOXYADENOSINE DIPHOSPHATE; MAGNESIUM ION; POTASSIUM ION; PYRUVATE KINASE; RECOMBINANT ENZYME; THYMIDINE DIPHOSPHATE;

AFFINITY CHROMATOGRAPHY; CARBOXY TERMINAL SEQUENCE; CENTRIFUGATION; COLUMN CHROMATOGRAPHY; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; FRACTIONATION; GEL FILTRATION; INCUBATION TEMPERATURE; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; MUSCLE; NONHUMAN; OPEN READING FRAME; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RABBIT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STORAGE; SYNTHESIS;

ANIMALS; HYDROGEN-ION CONCENTRATION; KINETICS; MUSCLES; PHOSPHOENOLPYRUVATE; PYRUVATE KINASE; RABBITS; THYMINE NUCLEOTIDES;

EID: 84881629939     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/hsz-2012-0334     Document Type: Conference Paper

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