Total biosynthesis of deoxynucleoside triphosphates using deoxynucleoside monophosphate kinases for PCR application

Biotechnology and Bioengineering

Volumn 98, Issue 1, 2007, Pages 1-11

  Bao, Jie ^a,b   Ryu, Dewey D Y ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Deoxynucleoside monophosphate kinases (dNMP kinases); Deoxynucleoside triphosphates (dNTP); DNA biosynthesis; PCR application; Total biosynthesis

Indexed keywords

DEOXYNUCLEOSIDE MONOPHOSPHATE KINASES (DNMP KINASES); DEOXYNUCLEOSIDE TRIPHOSPHATES (DNTP); DNA BIOSYNTHESIS; PCR APPLICATION; TOTAL BIOSYNTHESIS;

BIOSYNTHESIS; ENZYMES; ESCHERICHIA COLI; PHOSPHORYLATION; REACTION KINETICS;

PHOSPHATES;

DEOXYNUCLEOSIDE DIPHOSPHATE; DEOXYNUCLEOSIDE MONOPHOSPHATE KINASE; DEOXYNUCLEOSIDE TRIPHOSPHATE; MAGNESIUM; PHOSPHOTRANSFERASE; POTASSIUM; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BIOTECHNOLOGY; CONTROLLED STUDY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; FEASIBILITY STUDY; GENE AMPLIFICATION; GENE ISOLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PHOSPHORYLATION; POLYMERASE CHAIN REACTION;

CLONING, MOLECULAR; DEOXYRIBONUCLEOTIDES; ESCHERICHIA COLI; PHOSPHOTRANSFERASES (PHOSPHATE GROUP ACCEPTOR); POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING;

EID: 34548221584     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21498     Document Type: Article

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