Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin α(100-109) in sera of mice infected with bacteria

Journal of Immunological Methods

Volumn 395, Issue 1-2, 2013, Pages 54-62

  Samara, Pinelopi ^a   Kalbacher, Hubert ^b   Ioannou, Kyriaki ^a   Radu, Dorel L ^c   Livaniou, Evangelia ^d   Promponas, Vasilis J ^e   Voelter, Wolfgang ^b   Tsitsilonis, Ourania ^a  

^a UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c NATIONAL INSTITUTE FOR RESEARCH AND DEVELOPMENT IN MICROTECHNOLOGIES   (Romania)

^d INSTITUTE OF NANOSCIENCE AND NANOTECHNOLOGY   (Greece)

^e UNIVERSITY OF CYPRUS   (Cyprus)

Author keywords

Bacterial infection; ELISA; Immunoreactive peptide; In vivo model; Prothymosin alpha

Indexed keywords

BACTERIAL PROTEIN; POLYCLONAL ANTIBODY; PROTHYMOSIN ALPHA;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; BACTERIAL INFECTION; CARBOXY TERMINAL SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; STREPTOCOCCUS PYOGENES;

2,2′-AZINO-BIS (3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID); ABTS; BACTERIAL INFECTION; BOVINE SERUM ALBUMIN; BSA; CFA; CFU; COLONY-FORMING UNIT; COMPLETE FREUND'S ADJUVANT; ELISA; ENZYME-LINKED IMMUNOSORBENT ASSAY; HORSERADISH PEROXIDASE; HRP; IG; IMMUNOGLOBULIN; IMMUNOREACTIVE PEPTIDE; IN VIVO MODEL; KEYHOLE LIMPET HEMOCYANIN; KLH; PBS; PHOSPHATE BUFFERED SALINE; PROTΑ; PROTHYMOSIN ALPHA; RADIOIMMUNOASSAY; RIA; S. PYOGENES; STREPTOCOCCUS PYOGENES.;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODY SPECIFICITY; APOPTOSIS; COMPUTER SIMULATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; HUMANS; MALE; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; PEPTIDE FRAGMENTS; PROTEIN PRECURSORS; RABBITS; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS PYOGENES; THYMOSIN;

BACTERIA (MICROORGANISMS); MUS; ORYCTOLAGUS CUNICULUS; STREPTOCOCCUS PYOGENES;

EID: 84881557906     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.06.011     Document Type: Article

References (51)

1. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25:3389.
2. Baxevanis C.N., Voutsas I.F., Tsitsilonis O.E. Toll-like receptor agonists: current status and future perspective on their utility as adjuvants in improving anticancer vaccination strategies. Immunotherapy 2013, 5:497.
3. Benson D.A., Cavanaugh M., Clark K., Karsch-Mizrachi I., Lipman D.J., Ostell J., Sayers E.W. GenBank. Nucleic Acids Res. 2013, 41:D36. (Database issue).
4. Bianchi M.E. DAMPs, PAMPs and alarmins: all we need to know about danger. J. Leukoc. Biol. 2007, 81:1.
5. Chiappini N., Seubert A., Telford J.L., Grandi G., Serruto D., Margarit I., Janulczyk R. Streptococcus pyogenes SpyCEP influences host-pathogen interactions during infection in a murine air pouch model. PLoS One 2012, 7:e40411.
6. Cordero O.J., Sarandeses C., López-Rodríguez J.L., Nogueira M. The presence and cytotoxicity of CD16+ CD2- subset from PBL and NK cells in long-term IL-2 cultures enhanced by prothymosin-alpha. Immunopharmacology 1995, 29:215.
7. Costopoulou D., Leondiadis L., Czarnecki J., Ferderigos N., Ithakissios D.S., Livaniou E., Evangelatos G.P. Direct ELISA method for the specific determination of prothymosin alpha in human specimens. J. Immunoassay 1998, 19:295.
8. Crooks G.E., Hon G., Chandonia J.M., Brenner S.E. WebLogo: a sequence logo generator. Genome Res. 2004, 14:1188.
9. Dix M.M., Simon G.M., Cravatt B.F. Global mapping of the topography and magnitude of proteolytic events in apoptosis. Cell 2008, 134:679.
10. Dominguez F., Magdalena C., Cancio E., Roson E., Paredes J., Loidi L., Zalvide J., Fraga M., Forteza J., Regueiro B.J., Puente J.L. Tissue concentrations of prothymosin alpha: a novel proliferation index of primary breast cancer. Eur. J. Cancer 1993, 29A:893.
11. Eckert K., Grünberg E., Garbin F., Maurer H.R. Preclinical studies with prothymosin alpha1 on mononuclear cells from tumor patients. Int. J. Immunopharmacol. 1997, 19:493.
12. Enkemann S.A., Wang R.H., Trumbore M.W., Berger S.L. Functional discontinuities in prothymosin alpha caused by caspase cleavage in apoptotic cells. J. Cell. Physiol. 2000, 182:256.
13. Evstafieva A.G., Belov G.A., Kalkum M., Chichkova N.V., Bogdanov A.A., Agol V.I., Vartapetian A.B. Prothymosin alpha fragmentation in apoptosis. FEBS Lett. 2000, 467:150.
14. Evstafieva A.G., Belov G.A., Rubtsov Y.P., Kalkum M., Joseph B., Chichkova N.V., Sukhacheva E.A., Bogdanov A.A., Pettersson R.F., Agol V.I., Vartapetian A.B. Apoptosis-related fragmentation, translocation, and properties of human prothymosin alpha. Exp. Cell Res. 2003, 284:211.
15. Finn R.D., Clements J., Eddy S.R. HMMER web server: interactive sequence similarity searching. Nucleic Acids Res. 2011, 39:W29. (Web Server issue).
16. Flicek P., Amode M.R., Barrell D., Beal K., Brent S., Carvalho-Silva D., Clapham P., Coates G., Fairley S., Fitzgerald S., Gil L., Gordon L., Hendrix M., Hourlier T., Johnson N., Kähäri A.K., Keefe D., Keenan S., Kinsella R., Komorowska M., Koscielny G., Kulesha E., Larsson P., Longden I., McLaren W., Muffato M., Overduin B., Pignatelli M., Pritchard B., Riat H.S., Ritchie G.R., Ruffier M., Schuster M., Sobral D., Tang Y.A., Taylor K., Trevanion S., Vandrovcova J., White S., Wilson M., Wilder S.P., Aken B.L., Birney E., Cunningham F., Dunham I., Durbin R., Fernández-Suarez X.M., Harrow J., Herrero J., Hubbard T.J., Parker A., Proctor G., Spudich G., Vogel J., Yates A., Zadissa A., Searle S.M. Ensembl. Nucleic Acids Res 2012, 40:D84. (Database issue).
17. Fukao T. Immune system paralysis by anthrax lethal toxin: the roles of innate and adaptive immunity. Lancet Infect. Dis. 2004, 4:166.
18. Haritos A.A., Horecker B.L. A radioimmunoassay for thymosin alpha 1 that detects the native polypeptide, prothymosin alpha. J. Immunol. Methods 1985, 81:199.
19. Hilbi H., Zychlinsky A., Sansonetti P.J. Macrophage apoptosis in microbial infections. Parasitology 1997, 115:S79.
20. Ioannou K., Samara P., Livaniou E., Derhovanessian E., Tsitsilonis O.E. Prothymosin alpha: a ubiquitous polypeptide with potential use in cancer diagnosis and therapy. Cancer Immunol. Immunother. 2012, 61:599.
21. Ioannou K., Derhovanessian E., Samara P., Voutsas I., Voelter W., Kalbacher H., Pawelec G., Tsitsilonis O. Prothymosin alpha and its carboxy-terminal decapeptide proTalpha(100-109) induce Th1-type tumor-specific immune responses. Eur. J. Cancer 2012, 48S5:S259. (1078).
22. Jou Y.C., Tsai Y.S., Hsieh H.Y., Chen S.Y., Tsai H.T., Chen K.J., Wang S.T., Shiau A.L., Wu C.L., Tzai T.S. Plasma thymosin-α1 level as a potential biomarker in urothelial and renal cell carcinoma. Urol. Oncol 2013, (in press). 10.1016/j.urolonc.2012.03.011.
23. Kawashima Y., Fukutomi T., Tomonaga T., Takahashi H., Nomura F., Maeda T., Kodera Y. High-yield peptide-extraction method for the discovery of subnanomolar biomarkers from small serum samples. J. Proteome Res. 2010, 9:1694.
24. Klimentzou P., Paravatou-Petsotas M., Zikos C., Beck A., Skopeliti M., Czarnecki J., Tsitsilonis O., Voelter W., Livaniou E., Evangelatos G.P. Development and immunochemical evaluation of antibodies Y for the poorly immunogenic polypeptide prothymosin alpha. Peptides 2006, 27:183.
25. Klimentzou P., Drougou A., Fehrenbacher B., Schaller M., Voelter W., Barbatis C., Paravatou-Petsotas M., Livaniou E. Immunocytological and preliminary immunohistochemical studies of prothymosin alpha, a human cancer-associated polypeptide, with a well-characterized polyclonal antibody. J. Histochem. Cytochem. 2008, 56:1023.
26. Lancellotti M., Pereira R.F., Cury G.G., Hollanda L.M. Pathogenic and opportunistic respiratory bacteria-induced apoptosis. Braz. J. Infect. Dis. 2009, 13:226.
27. Lexmond W., der Mee J.v, Ruiter F., Platzer B., Stary G., Yen E.H., Dehlink E., Nurko S., Fiebiger E. Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum. J. Immunol. Methods 2011, 373:192.
28. Loidi L., Vidal A., Zalvide J.B., Puente J.L., Reyes F., Domínguez F. Development of ELISA to estimate thymosin alpha1, the N terminus of prothymosin alpha, in human tumors. Clin. Chem. 1997, 43:59.
29. Medina E., Goldmann O., Rohde M., Lengeling A., Chhatwal G.S. Genetic control of susceptibility to group A streptococcal infection in mice. J. Infect. Dis. 2001, 184:846.
30. Moody T.W., Leyton J., Zia F., Tuthill C., Badamchian M., Goldstein A.L. Thymosinalpha1 is chemopreventive for lung adenoma formation in A/J mice. Cancer Lett. 2000, 155:121.
31. Mosoian A., Teixeira A., Burns C.S., Sander L.E., Gusella G.L., He C., Blander J.M., Klotman P., Klotman M.E. Prothymosin-alpha inhibits HIV-1 via Toll-like receptor 4-mediated type I interferon induction. Proc. Natl. Acad. Sci. U.S.A. 2010, 107:10178.
32. Ngai P.K., Ackermann F., Wendt H., Savoca R., Bosshard H.R. Protein A antibody-capture ELISA (PACE): an ELISA format to avoid denaturation of surface-adsorbed antigens. J. Immunol. Methods 1993, 158:267.
33. Promponas V.J., Enright A.J., Tsoka S., Kreil D.P., Leroy C., Hamodrakas S., Sander C., Ouzounis C.A. CAST: an iterative algorithm for the complexity analysis of sequence tracts. Bioinformatics 2000, 16:915.
34. Pruitt K.D., Tatusova T., Brown G.R., Maglott D.R. NCBI Reference Sequences (RefSeq): current status, new features and genome annotation policy. Nucleic Acids Res. 2012, 40:D130. (Database issue).
35. Ravichandran K.S. Beginnings of a good apoptotic meal: the find-me and eat-me signaling pathways. Immunity 2011, 35:445.
36. Samara P., Ioannou K., Neagu M., Arnogiannaki N., Ardavanis A., Voelter W., Tsitsilonis O. The C-terminal decapeptide of prothymosin α is responsible for its stimulatory effect on the functions of human neutrophils in vitro. Int. Immunopharmacol. 2013, 15:50.
37. Singer M. Biomarkers in sepsis. Curr. Opin. Pulm. Med. 2013, 19:305.
38. Skopeliti M., Voutsas I.F., Klimentzou P., Tsiatas M.L., Beck A., Bamias A., Moraki M., Livaniou E., Neagu M., Voelter W., Tsitsilonis O.E. The immunologically active site of prothymosin alpha is located at the carboxy-terminus of the polypeptide Evaluation of its in vitro effects in cancer patients. Cancer Immunol. Immunother. 2006, 55:1247.
39. Skopeliti M., Iconomidou V.A., Derhovanessian E., Pawelec G., Voelter W., Kalbacher H., Hamodrakas S.J., Tsitsilonis O.E. Prothymosin alpha immunoactive carboxyl-terminal peptide TKKQKTDEDD stimulates lymphocyte reactions, induces dendritic cell maturation and adopts a beta-sheet conformation in a sequence-specific manner. Mol. Immunol. 2009, 46:784.
40. Sukhacheva E.A., Evstafieva A.G., Fateeva T.V., Shakulov V.R., Efimova N.A., Karapetian R.N., Rubtsov Y.P., Vartapetian A.B. Sensing prothymosin alpha origin, mutations and conformation with monoclonal antibodies. J. Immunol. Methods 2002, 266:185.
41. Timmer A.M., Timmer J.C., Pence M.A., Hsu L.C., Ghochani M., Frey T.G., Karin M., Salvesen G.S., Nizet V. Streptolysin O promotes group A Streptococcus immune evasion by accelerated macrophage apoptosis. J. Biol. Chem. 2009, 284:862.
42. Tsitsiloni O.E., Heimer E., Felix A., Yialouris P.P., Vamvoukakis J., Voelter W., Haritos A.A. Radioimmunoassays for the C-terminus of prothymosin alpha and the N-terminus of parathymosin alpha for the measurement of the levels of alpha-thymosins in human cancer. J. Immunol. Methods 1994, 169:163.
43. Tzai T.S., Tsai Y.S., Shiau A.L., Wu C.L., Shieh G.S., Tsai H.T. Urine prothymosin-alpha as novel tumor marker for detection and follow-up of bladder cancer. Urology 2006, 67:294.
44. Ulett G.C., Ketheesan N., Hirst R.G. Cytokine gene expression in innately susceptible BALB/c mice and relatively resistant C57BL/6 mice during infection with virulent Burkholderia pseudomallei. Infect. Immun. 2000, 68:2034.
45. UniProt Consortium Reorganizing the protein space at the Universal Protein Resource (UniProt). Nucleic Acids Res. 2012, 40:D71. (Database issue).
46. Van den Berg S., Laman J.D., Boon L., ten Kate M.T., de Knegt G.J., Verdijk R.M., Verbrugh H.A., Nouwen J.L., Bakker-Woudenberg I.A. Distinctive cytokines as biomarkers predicting fatal outcome of severe Staphylococcus aureus bacteremia in mice. PLoS One 2013, 8:e59107.
47. Voutsas I.F., Baxevanis C.N., Gritzapis A.D., Missitzis I., Stathopoulos G.P., Archodakis G., Banis C., Voelter W., Papamichail M. Synergy between interleukin-2 and prothymosin alpha for the increased generation of cytotoxic T lymphocytes against autologous human carcinomas. Cancer Immunol. Immunother. 2000, 49:449.
48. Voutsas I.F., Pistamaltzian N., Tsiatas M.L., Skopeliti M., Katsila T., Mavrothalassiti I., Spyrou S., Dimopoulos M.A., Tsitsilonis O.E., Bamias A. Ovarian malignant ascites-derived lymphocytes stimulated with prothymosin α or its immunoactive decapeptide lyse autologous tumour cells in vitro and retard tumour growth in SCID mice. Eur. J. Cancer 2013, 49:1706.
49. Waterhouse A.M., Procter J.B., Martin D.M., Clamp M., Barton G.J. Jalview version 2-a multiple sequence alignment editor and analysis workbench. Bioinformatics 2009, 25:1189.
50. Yialouris P.P., Evangelatos G.P., Soteriadis-Vlahos C., Heimer E.P., Felix A.M., Tsitsiloni O.E., Haritos A.A. The identification of prothymosin alpha-like material in vertebrate lymphoid organs by a radioimmunoassay for the N-terminal decapeptide. J. Immunol. Methods 1988, 106:267.
51. Zaidi N., Herrmann T., Baechle D., Schleicher S., Gogel J., Driessen C., Voelter W., Kalbacher H. A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles. FEBS J. 2007, 274:3138.