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Volumn 15, Issue 8, 2013, Pages 387-392

High-throughput thermal scanning for protein stability: Making a good technique more robust

Author keywords

differential scanning fluorimetry; formulation; protein stability; protein unfolding; thermal shift assay; thermofluor

Indexed keywords

ARTICLE; HIGH THROUGHPUT SCREENING; LIGHT; METHODOLOGY; PROTEIN STABILITY; RADIATION SCATTERING; TEMPERATURE; VALIDATION STUDY;

EID: 84881486856     PISSN: 21568952     EISSN: None     Source Type: Journal    
DOI: 10.1021/co400013v     Document Type: Article
Times cited : (62)

References (19)
  • 2
    • 0030218803 scopus 로고    scopus 로고
    • SYPRO Orange and SYPRO Red Protein Gel Stains: One-Step Fluorescent Staining of Denaturing Gels for Detection of Nanogram Levels of Protein
    • Steinberg, T. H.; Jones, L. J.; Haugland, R. P.; Singer, V. L. SYPRO Orange and SYPRO Red Protein Gel Stains: One-Step Fluorescent Staining of Denaturing Gels for Detection of Nanogram Levels of Protein Anal. Biochem. 1996, 239 (2) 223-237
    • (1996) Anal. Biochem. , vol.239 , Issue.2 , pp. 223-237
    • Steinberg, T.H.1    Jones, L.J.2    Haugland, R.P.3    Singer, V.L.4
  • 3
    • 79951895571 scopus 로고    scopus 로고
    • Formulation Development of Therapeutic Monoclonal Antibodies Using High-Throughput Fluorescence and Static Light Scattering Techniques: Role of Conformational and Colloidal Stability
    • Goldberg, D. S.; Bishop, S. M.; Shah, A. U.; Sathish, H. A. Formulation Development of Therapeutic Monoclonal Antibodies Using High-Throughput Fluorescence and Static Light Scattering Techniques: Role of Conformational and Colloidal Stability J. Pharm. Sci. 2011, 100 (4) 1306-1315
    • (2011) J. Pharm. Sci. , vol.100 , Issue.4 , pp. 1306-1315
    • Goldberg, D.S.1    Bishop, S.M.2    Shah, A.U.3    Sathish, H.A.4
  • 4
    • 37249005205 scopus 로고    scopus 로고
    • The Use of Differential Scanning Fluorimetry to Detect Ligand Interactions That Promote Protein Stability
    • Niesen, F. H.; Berglund, H.; Vedadi, M. the Use of Differential Scanning Fluorimetry to Detect Ligand Interactions That Promote Protein Stability Nat. Protoc. 2007, 2 (9) 2212-2221
    • (2007) Nat. Protoc. , vol.2 , Issue.9 , pp. 2212-2221
    • Niesen, F.H.1    Berglund, H.2    Vedadi, M.3
  • 6
    • 79960694785 scopus 로고    scopus 로고
    • The Combined Use of the Thermofluor Assay and Thermoq Analytical Software for the Determination of Protein Stability and Buffer Optimization As an Aid in Protein Crystallization
    • Phillips, K.; de la Pena, A. H. The Combined Use of the Thermofluor Assay and Thermoq Analytical Software for the Determination of Protein Stability and Buffer Optimization As an Aid in Protein Crystallization Curr. Protoc. Mol. Biol. 2011, 10, 10-28
    • (2011) Curr. Protoc. Mol. Biol. , vol.10 , pp. 10-28
    • Phillips, K.1    De La Pena, A.H.2
  • 7
    • 77956191215 scopus 로고    scopus 로고
    • Biophysical Characterization of Recombinant Proteins: A Key to Higher Structural Genomics Success
    • Vedadi, M.; Arrowsmith, C. H.; Allali-Hassani, A.; Senisterra, G.; Wasney, G. A. Biophysical Characterization of Recombinant Proteins: A Key to Higher Structural Genomics Success J. Struct. Biol. 2010, 172 (1) 107-119
    • (2010) J. Struct. Biol. , vol.172 , Issue.1 , pp. 107-119
    • Vedadi, M.1    Arrowsmith, C.H.2    Allali-Hassani, A.3    Senisterra, G.4    Wasney, G.A.5
  • 8
    • 77949789023 scopus 로고    scopus 로고
    • High Throughput Thermostability Screening of Monoclonal Antibody Formulations
    • He, F.; Hogan, S.; Latypov, R. F.; Narhi, L. O.; Razinkov, V. I. High Throughput Thermostability Screening of Monoclonal Antibody Formulations J. Pharm. Sci. 2010, 99 (4) 1707-1720
    • (2010) J. Pharm. Sci. , vol.99 , Issue.4 , pp. 1707-1720
    • He, F.1    Hogan, S.2    Latypov, R.F.3    Narhi, L.O.4    Razinkov, V.I.5
  • 9
    • 60649093306 scopus 로고    scopus 로고
    • High Throughput Methods of Assessing Protein Stability and Aggregation
    • Senisterra, G. A.; Finerty, P. J. High Throughput Methods of Assessing Protein Stability and Aggregation Mol. BioSyst. 2009, 5 (3) 217-223
    • (2009) Mol. BioSyst. , vol.5 , Issue.3 , pp. 217-223
    • Senisterra, G.A.1    Finerty, P.J.2
  • 11
    • 79951892441 scopus 로고    scopus 로고
    • Screening of Monoclonal Antibody Formulations Based on High-Throughput Thermostability and Viscosity Measurements: Design of Experiment and Statistical Analysis
    • He, F.; Woods, C. E.; Trilisky, E.; Bower, K. M.; Litowski, J. R.; Kerwin, B. A.; Becker, G. W.; Narhi, L. O.; Razinkov, V. I. Screening of Monoclonal Antibody Formulations Based on High-Throughput Thermostability and Viscosity Measurements: Design of Experiment and Statistical Analysis J. Pharm. Sci. 2011, 100 (4) 1330-1340
    • (2011) J. Pharm. Sci. , vol.100 , Issue.4 , pp. 1330-1340
    • He, F.1    Woods, C.E.2    Trilisky, E.3    Bower, K.M.4    Litowski, J.R.5    Kerwin, B.A.6    Becker, G.W.7    Narhi, L.O.8    Razinkov, V.I.9
  • 14
    • 77951818493 scopus 로고    scopus 로고
    • Differential Scanning Fluorimetry Measurement of Protein Stability Changes upon Binding to Glycosaminoglycans: A Screening Test for Binding Specificity
    • Uniewicz, K. A.; Ori, A.; Xu, R. Y.; Ahmed, Y.; Wilkinson, M. C.; Fernig, D. G.; Yates, E. A. Differential Scanning Fluorimetry Measurement of Protein Stability Changes upon Binding to Glycosaminoglycans: A Screening Test for Binding Specificity Anal. Chem. 2010, 82 (9) 3796-3802
    • (2010) Anal. Chem. , vol.82 , Issue.9 , pp. 3796-3802
    • Uniewicz, K.A.1    Ori, A.2    Xu, R.Y.3    Ahmed, Y.4    Wilkinson, M.C.5    Fernig, D.G.6    Yates, E.A.7
  • 15
    • 33748929006 scopus 로고    scopus 로고
    • Thermofluor-Based High-Throughput Stability Optimization of Proteins for Structural Studies
    • Ericsson, U. B.; Hallberg, B. M.; DeTitta, G. T.; Dekker, N.; Nordlund, P. Thermofluor-Based High-Throughput Stability Optimization of Proteins for Structural Studies Anal. Biochem. 2006, 357 (2) 289-298
    • (2006) Anal. Biochem. , vol.357 , Issue.2 , pp. 289-298
    • Ericsson, U.B.1    Hallberg, B.M.2    Detitta, G.T.3    Dekker, N.4    Nordlund, P.5
  • 18
    • 0025129302 scopus 로고
    • Characterization of Precrystallization Aggregation of Canavalin by Dynamic Light Scattering
    • Kadima, W.; McPherson, A.; Dunn, M. F.; Jurnak, F. A. Characterization of Precrystallization Aggregation of Canavalin by Dynamic Light Scattering Biophys. J. 1990, 57 (1) 125-132
    • (1990) Biophys. J. , vol.57 , Issue.1 , pp. 125-132
    • Kadima, W.1    McPherson, A.2    Dunn, M.F.3    Jurnak, F.A.4
  • 19
    • 36749117497 scopus 로고
    • Photon Correlation Spectroscopy of Particle Distributions
    • Gulari, E.; Gulari, E.; Tsunashima, Y.; Chu, B. Photon Correlation Spectroscopy of Particle Distributions J. Chem. Phys. 1979, 70 (8) 3965-3972
    • (1979) J. Chem. Phys. , vol.70 , Issue.8 , pp. 3965-3972
    • Gulari, E.1    Gulari, E.2    Tsunashima, Y.3    Chu, B.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.