Optimized expression of the dirigent protein AtDIR6 in Pichia pastoris and impact of glycosylation on protein structure and function

Applied Microbiology and Biotechnology

Volumn 97, Issue 16, 2013, Pages 7215-7227

  Kazenwadel, Christian ^a   Klebensberger, Janosch ^a   Richter, Sven ^a   Pfannstiel, Jens ^b   Gerken, Uwe ^b   Pickel, Benjamin ^b   Schaller, Andreas ^b   Hauer, Bernhard ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

Dirigent protein; Fermentation; Lignan biosynthesis; N glycosylation; Phenoxy radical coupling; Pichia pastoris

Indexed keywords

BIOCHEMICAL CHARACTERIZATION; FED-BATCH FERMENTATION; HETEROLOGOUS EXPRESSION; N-GLYCOSYLATION; N-GLYCOSYLATION SITES; PHENOXY RADICALS; PICHIA PASTORIS; STEREOSELECTIVE FORMATION;

BIOSYNTHESIS; CIRCULAR DICHROISM SPECTROSCOPY; ESTERIFICATION; FERMENTATION; GLYCOSYLATION; PLANT CELL CULTURE; POLYSACCHARIDES; YEAST;

PROTEINS;

FUNGAL PROTEIN; GLYCAN;

ALCOHOL; COUPLING; FERMENTATION; PLANT; PROTEIN; RADICAL; SOLUBILITY;

ARTICLE; BIOTRANSFORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DEGLYCOSYLATION; FED BATCH FERMENTATION; GLYCOSYLATION; NONHUMAN; PICHIA PASTORIS; PLANT CELL; PLANT CELL CULTURE; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; SOLANUM; SOLUBILITY; SPECTROSCOPY;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; CIRCULAR DICHROISM; FURANS; GENE EXPRESSION; GLYCOSYLATION; LIGNANS; PHENOLS; PICHIA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

LYCOPERSICON PERUVIANUM; PICHIA PASTORIS;

EID: 84881226999     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4579-x     Document Type: Article

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