MHC genotyping of non-model organisms using next-generation sequencing: A new methodology to deal with artefacts and allelic dropout

BMC Genomics

Volumn 14, Issue 1, 2013, Pages

  Sommer, Simone ^a   Courtiol, Alexandre ^a   Mazzoni, Camila J ^a,b  

^a LEIBNIZ INSTITUTE FOR ZOO AND WILDLIFE RESEARCH   (Germany)

^b Berlin Center for Genomics in Biodiversity Research   (Germany)

Author keywords

454 pyrosequencing; Allelic dropout; Amplification efficiency; Delomys sublineatus; Major histocompatibility complex; Molecular cloning; Next generation sequencing; PCR and sequencing artefacts; Rodent

Indexed keywords

MAJOR HISTOCOMPATIBILITY ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2;

ALLELE; ALLELIC IMBALANCE; ANIMAL TISSUE; ARTICLE; ARTIFACT REDUCTION; CRICETIDAE; DELOMYS SUBLINEATUS; GENE; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC VARIABILITY; GENOTYPE; GENOTYPING TECHNIQUE; INTERMETHOD COMPARISON; MAJOR HISTOCOMPATIBILITY COMPLEX; MHC GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROCESS DEVELOPMENT; PYROSEQUENCING; VALIDATION PROCESS;

ALLELES; ANIMALS; ARTIFACTS; ARVICOLINAE; CLONING, MOLECULAR; DATABASES, GENETIC; GENOTYPING TECHNIQUES; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MAJOR HISTOCOMPATIBILITY COMPLEX;

DELOMYS SUBLINEATUS; RODENTIA;

EID: 84881126426     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-14-542     Document Type: Article

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