Large-scale purification of pharmaceutical-grade plasmid DNA using tangential flow filtration and multi-step chromatography

Journal of Bioscience and Bioengineering

Volumn 116, Issue 3, 2013, Pages 281-286

  Sun, Bo ^a   Yu, Xiang Hui ^a   Yin, Yuhe ^b   Liu, Xintao ^b   Wu, Yongge ^a   Chen, Yan ^a   Zhang, Xizhen ^a   Jiang, Chunlai ^a   Kong, Wei ^a  

^a JILIN UNIVERSITY   (China)

^b CHANGCHUN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Alkaline lysis; Chromatography; DNA vaccine; Gene therapy; Purification

Indexed keywords

AGAROSE GEL ELECTROPHORESIS; ALKALINE LYSIS; ANION EXCHANGE CHROMATOGRAPHY; DNA VACCINE; EFFICIENT PRODUCTION; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; PHARMACEUTICAL GRADE PLASMID DNA; TANGENTIAL FLOW FILTRATIONS;

BIOMOLECULES; CHROMATOGRAPHY; ELECTROPHORESIS; GENE THERAPY; LIQUID CHROMATOGRAPHY; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; VACCINES;

NUCLEIC ACIDS;

ENDOTOXIN; PLASMID DNA;

AGAR GEL ELECTROPHORESIS; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACTERIAL CELL; DNA CONTENT; FILTRATION; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; LYSIS; NONHUMAN; PROTEIN CONTENT; PURIFICATION; TANGENTIAL FLOW FILTRATION;

BACTERIA (MICROORGANISMS);

ALKALINE LYSIS; CHROMATOGRAPHY; DNA VACCINE; GENE THERAPY; PURIFICATION;

CHROMATOGRAPHY; CHROMOSOMES, BACTERIAL; DNA; DRUG CONTAMINATION; FILTRATION; GENETIC THERAPY; PLASMIDS; RNA; VACCINES, DNA;

EID: 84881114899     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.03.015     Document Type: Article

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