The use of tissue-nonspecific alkaline phosphatase (TNAP) and PHOSPHO1 inhibitors to affect mineralization by cultured cells

Methods in Molecular Biology

Volumn 1053, Issue , 2013, Pages 125-134

  Kiffer Moreira, Tina ^a   Narisawa, Sonoko ^a  

^a BURNHAM INSTITUTE   (United States)

Author keywords

Cell culture; High throughput screening; Kinetic studies; Pharmacological inhibitors; PHOSPHO1; Small molecules; TNAP; Vascular smooth muscle cells

Indexed keywords

CALCIUM; PHOSPHATASE; PHOSPHO1 PROTEIN; PROTEIN INHIBITOR; RECOMBINANT PROTEIN; TISSUE NONSPECIFIC ALKALINE PHOSPHATASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL TISSUE; AORTA; ARTICLE; COLORIMETRY; MINERALIZATION; MOUSE; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STRUCTURE ACTIVITY RELATION; VASCULAR SMOOTH MUSCLE;

ALKALINE PHOSPHATASE; ANIMALS; CELLS, CULTURED; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; HIGH-THROUGHPUT SCREENING ASSAYS; MICE; MUSCLE, SMOOTH, VASCULAR; MYOCYTES, SMOOTH MUSCLE; OSTEOBLASTS; PHOSPHORIC MONOESTER HYDROLASES; SKULL; STRUCTURE-ACTIVITY RELATIONSHIP; VASCULAR CALCIFICATION;

EID: 84881103103     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-562-0_8     Document Type: Article

References (16)

1. Towler DA (2008) Vascular calcifi cation: A perspective on an imminent disease epidemic. IBMS Bonekey 5:41-58
2. O'Neill WC, Sigrist MK, McIntyre CW (2010) Plasma pyrophosphate and vascular calcifi cation in chronic kidney disease. Nephrol Dial Transplant 25:187-191
3. Narisawa S, Harmey D, Yadav MC et al (2007) Novel inhibitors of alkaline phosphatase suppress vascular smooth muscle cell calcifi cation. J Bone Miner Res 22:1700-1710
4. Lomashvili KA, Garg P, Narisawa S et al (2008) Upregulation of alkaline phosphatase and pyrophosphate hydrolysis: Potential mechanism for uremic vascular calcifi cation. Kidney Int 73:1024-1030
5. Villa-Bellosta R, Wang X, Millán JL et al (2011) Extracellular pyrophosphate metabolism and calcifi cation in vascular smooth muscle. Am J Physiol Heart Circ Physiol 301:H61-H68
6. Hessle L, Johnson KA, Anderson HC et al (2002) Tissue-nonspecifi c alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization. Proc Natl Acad Sci U S A 99: 9445-9449
7. Houston B, Seawright E, Jefferies D et al (1999) Identifi cation and cloning of a novel phosphatase expressed at high levels in differentiating growth plate chondrocytes. Biochim Biophys Acta 1448:500-506
8. Stewart AJ, Schmid R, Blindauer CA et al (2003) Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily. Protein Eng 16:889-895
9. Houston B, Stewart AJ, Farquharson C (2004) PHOSPHO1-A novel phosphatase specifi cally expressed at sites of mineralisation in bone and cartilage. Bone 34:629-637
10. Stewart AJ, Roberts SJ, Seawright E et al (2006) The presence of PHOSPHO1 in matrix vesicles and its developmental expression prior to skeletal mineralization. Bone 39:1000-1007
11. Roberts S, Narisawa S, Harmey D et al (2007) Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization. J Bone Miner Res 22:617-627
12. Roberts SJ, Stewart AJ, Sadler PJ et al (2004) Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities. Biochem J 382:59-65
13. Hsu HH, Camacho NP (1999) Isolation of calcifi able vesicles from human atherosclerotic aortas. Atherosclerosis 143:353-362
14. Kapustin AN, Davies JD, Reynolds JL et al (2011) Calcium regulates key components of vascular smooth muscle cell-derived matrix vesicles to enhance mineralization. Circ Res 109:e1-e12
15. Kiffer-Moreira T, Yadav M, Zhu D et al (2013) Pharmacological inhibition of PHOSPHO1 suppresses vascular smooth muscle cell calcifi-cation. J Bone Miner Res 28(1):81-91
16. Dahl R, Sergienko EA, Su Y et al (2009) Discovery and validation of a series of aryl sulfonamides as selective inhibitors of tissuenonspecifi c alkaline phosphatase (TNAP). J Med Chem 52:6919-6925