Integrated RNA extraction and RT-PCR for semi-quantitative gene expression studies on a microfluidic device

Laboratory Investigation

Volumn 93, Issue 8, 2013, Pages 961-966

  Shaw, Kirsty J ^a   Hughes, Elizabeth M ^b   Dyer, Charlotte E ^c   Greenman, John ^c   Haswell, Stephen J ^c  

^a MANCHESTER METROPOLITAN UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF HULL   (United Kingdom)

^c UNIVERSITY OF HULL   (United Kingdom)

Author keywords

Diagnostic; Metabolism; Microfluidics; Point of care

Indexed keywords

3 METHYLCHOLANTHRENE; CYTOCHROME P450; CYTOCHROME P450 1A2; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DRUG SCREENING; ELUTION; GENE EXPRESSION; HOUSEKEEPING GENE; INTRON; LIVER CELL; MALE; MICROFLUIDIC ANALYSIS; MICROFLUIDICS; NONHUMAN; POINT OF CARE TESTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON TRANSPORT; QUALITATIVE ANALYSIS; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SOLID PHASE EXTRACTION;

ANIMALS; CYTOCHROME P-450 CYP1A2; GENE EXPRESSION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HEPATOCYTES; LIVER; MALE; MICROFLUIDIC ANALYTICAL TECHNIQUES; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA;

EID: 84881065768     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2013.76     Document Type: Article

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