Oligonucleotide immobilization using 10-(carbomethoxy)decyl- dimethylchlorosilane for mRNA isolation and cDNA synthesis on a microfluidic chip

Sensors and Actuators, B: Chemical

Volumn 155, Issue 2, 2011, Pages 437-445

  Hughes Chinkhota, Chantelle N ^a   Banda, Malathi ^b   Smolinski, Joseph M ^a   Thomas, Robert A ^b   Petibone, Dayton M ^b,c   Tucker, James D ^b   Auner, Gregory W ^a  

^a Wayne State University   (United States)

^b WAYNE STATE UNIVERSITY   (United States)

^c NCTR   (United States)

Author keywords

Microfluidic device; mRNA detection; Oligonucleotide immobilization; PCR; Silane

Indexed keywords

ACID EXTRACTION; BIOLOGICAL SAMPLES; CDNA SYNTHESIS; CELL LYSIS; CHIP INTEGRATION; CONTACT ANGLE GONIOMETRY; DOWNSTREAM APPLICATIONS; FUNCTIONALIZATIONS; GENE EXPRESSION ANALYSIS; LIQUID DEPOSITION; LYMPHOBLASTOID CELLS; MICRO FLUIDIC SYSTEM; MICRO-FLUIDIC DEVICES; MICROFLUIDIC CHIP; MRNA BINDING; MRNA DETECTION; MULTI-STEP; OLIGONUCLEOTIDE IMMOBILIZATION; ON CHIPS; OPTIMAL METHODS; PCR; QUANTITATIVE REAL TIME PCR; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; SAMPLE PREPARATION; SUBSTRATE DEPOSITION; SURFACE FUNCTIONALIZATION;

ATOMIC FORCE MICROSCOPY; BIOMOLECULES; CHEMICAL MODIFICATION; CONTACT ANGLE; FLUIDIC DEVICES; FLUORESCENCE MICROSCOPY; FUNCTIONAL POLYMERS; GENES; MICROFLUIDICS; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; SYNTHESIS (CHEMICAL); TRANSCRIPTION;

CELL IMMOBILIZATION;

EID: 79957859737     PISSN: 09254005     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.snb.2010.12.043     Document Type: Article

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