Rapid high-yield mRNA extraction for reverse-transcription PCR

Journal of Biochemical and Biophysical Methods

Volumn 70, Issue 3, 2007, Pages 507-509

  Wang, Chengming ^a   Kim, Teayoun ^a   Gao, Dongya ^a   Vaglenov, Alexander ^a   Kaltenboeck, Bernhard ^a  

^a AUBURN UNIVERSITY   (United States)

Author keywords

DNase; mRNA extraction; Oligo (dT); Reverse transcription PCR; Silica beads

Indexed keywords

DEOXYRIBONUCLEASE; MESSENGER RNA; SILICON DIOXIDE;

ANALYTIC METHOD; ARTICLE; CENTRIFUGATION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

ANIMALS; ARGINASE; LUNG; MICE; OLIGODEOXYRIBONUCLEOTIDES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SILICON DIOXIDE;

EID: 33847643695     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2006.10.003     Document Type: Article

References (5)

1. Ho S.B., Hyslop A., Albrecht R., Jacobson A., Spencer M., Rothenberger D.A., et al. Quantification of colorectal cancer micrometastases in lymph nodes by nested and real-time reverse transcriptase-PCR analysis for carcinoembryonic antigen. Clin Cancer Res 10 (2004) 5777-5784
2. Lai J.P., Yang J.H., Douglas S.D., Wang X., Riedel E., and Ho W.Z. Quantification of CCR5 mRNA in human lymphocytes and macrophages by real-time reverse transcriptase PCR assay. Clin Diagn Lab Immunol 10 (2003) 1123-1128
3. Moniotte S., Vaerman J.L., Kockx M.M., Larrouy D., Langin D., Noirhomme P., et al. Real-time RT-PCR for the detection of beta-adrenoceptor messenger RNAs in small human endomyocardial biopsies. J Mol Cell Cardiol 33 (2001) 2121-2133
4. Ramalho A.S., Beck S., Farinha C.M., Clarke L.A., Heda G.D., Steiner B., et al. Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts. J Cystic Fibros 3 (2004) 11-15
5. Wang C., Gao D., Vaglenov A., and Kaltenboeck B. One-step duplex reverse transcription PCRs simultaneously quantify analyte and housekeeping gene mRNAs. Biotechniques 36 (2004) 508-519