Gluco-oligomers initially formed by the reuteransucrase enzyme of Lactobacillus reuteri 121 incubated with sucrose and malto-oligosaccharides

Glycobiology

Volumn 23, Issue 9, 2013, Pages 1084-1096

  Dobruchowska, Justyna M ^a   Meng, Xiangfeng ^a   Leemhuis, Hans ^a   Gerwig, Gerrit J ^a,b   Dijkhuizen, Lubbert ^a   Kamerling, Johannis P ^a,b  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UTRECHT UNIVERSITY   (Netherlands)

Author keywords

D glucans; Glucanotransferase; Glucansucrase; Lactobacillus reuteri; Structural analysis

Indexed keywords

GLUCOSE; GLUCOSYLTRANSFERASE; MALTOSE; OLIGOMER; OLIGOSACCHARIDE; POLYMER; POLYSACCHARIDE; REUTERANSUCRASE; SUCROSE; UNCLASSIFIED DRUG;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACTERIUM CULTURE; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL STRUCTURE; CONTROLLED STUDY; DONOR; ENZYME SPECIFICITY; ENZYME SYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LACTOBACILLUS REUTERI; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN POLYMERIZATION; PROTON NUCLEAR MAGNETIC RESONANCE;

GLUCANOTRANSFERASE; GLUCANSUCRASE; LACTOBACILLUS REUTERI; STRUCTURAL ANALYSIS; Α-D-GLUCANS;

GLYCOSYLTRANSFERASES; LACTOBACILLUS REUTERI; OLIGOSACCHARIDES; SUCROSE;

EID: 84880959523     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwt048     Document Type: Article

References (25)

1. Bock K, Thøgersen H. 1982. Nuclear magnetic resonance spectroscopy in the study of mono- and oligosaccharides. Annu Rep NMR Spectrosc. 13: 1-57.
2. Brison Y, Laguerre S, Lefoulon F, Morel S, Monties N, Potocki-Véronèse G, Monsan P, Remaud-Simeon M. 2013. Branching pattern of gluco-oligosaccharides and 1.5 kDa dextran grafted by the α-1, 2 branching sucrase GBD-CD2. Carbohydr Polym. 94: 567-576.
3. Brison Y, Pijning T, Malbert Y, Fabre É, Mourey L, Morel S, Potocki-Véronèse G, Monsan P, Tranier S, Remaud-Siméon M, et al. 2012. Functional and structural characterization of α-(1 → 2) branching sucrase derived from DSR-E glucansucrase. J Biol Chem. 287: 7915-7924.
4. Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B. 2009. The Carbohydrate-Active EnZymes database (CAZy): An expert resource for glycogenomics. Nucleic Acids Res. 37: D233-D238.
5. Côté G, Sheng S, Dunlap CA. 2008. Alternansucrase acceptor products. Biocat Biotransform. 26: 161-168.
6. Dijkhuizen L, van der Maarel MJEC, Kamerling JP, Leemhuis RJ, Kralj S, Dobruchowska JM. 2010. Glucose-oligosaccharides comprising (α1 → 4) and (α1 → 6) glycosidic bonds, use thereof, and methods for providing them. Patent WO 2010/128859 A2.
7. Dobruchowska JM, Gerwig GJ, Kralj S, Grijpstra P, Leemhuis H, Dijkhuizen L, Kamerling JP. 2012. Structural characterization of linear isomalto-/maltooligomer products synthesized by the novel GTFB 4, 6-α-glucanotransferase enzyme from Lactobacillus reuteri 121. Glycobiology. 22: 517-528.
8. Jansson P-E, Stenutz R, Widmalm G. 2006. Sequence determination of oligosaccharides and regular polysaccharides using NMR spectroscopy and a novel web-based version of the computer program. Carbohydr Res. 341: 1003-1010.
9. Kralj S, Grijpstra P, van Leeuwen SS, Leemhuis H, Dobruchowska JM, van der Kaaij RM, Malik A, Oetari A, Kamerling JP, Dijkhuizen L. 2011. 4, 6-α-Glucanotransferase, a novel enzyme that structurally and functionally provides an evolutionary link between glycoside hydrolase enzyme families 13 and 70. Appl Environ Microbiol. 77: 8154-8163.
10. Kralj S, van Geel-Schutten GH, Dondorff MMG, Kirsanovs S, van der Maarel MJEC, Dijkhuizen L. 2004. Glucan synthesis in the genus Lactobacillus: Isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains. Microbiology. 150: 3681-3690.
11. Kralj S, van Geel-Schutten GH, Rahaoui H, Leer RJ, Faber EJ, van der Maarel MJEC, Dijkhuizen L. 2002. Molecular characterization of a novel glucosyltransferase from Lactobacillus reuteri strain 121 synthesizing a unique, highly branched glucan with α-(1 → 4) and α-(1 → 6) glucosidic bonds. Appl Environ Microbiol. 68: 4283-4291.
12. Kralj S, van Geel-Schutten GH, van der Maarel MJEC, Dijkhuizen L. 2004. Biochemical and molecular characterization of Lactobacillus reuteri 121 reuteransucrase. Microbiology. 150: 2099-2112.
13. Leemhuis H, Dijkman WP, Dobruchowska JM, Pijning T, Grijpstra P, Kralj S, Kamerling JP, Dijkhuizen L. 2013. 4, 6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily. Appl Microbiol Biotechnol. 97: 181-193.
14. Leemhuis H, Pijning T, Dobruchowska JM, van Leeuwen SS, Kralj S, Dijkstra BW, Dijkhuizen L. 2013. Glucansucrases: Three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications. J Biotechnol. 163: 250-272.
15. Levitt MH, Freeman R, Frenkiel J. 1982. Broadband heteronuclear decoupling. J Magn Reson. 47: 328-330.
16. Monchois V, Willemot R-M, Monsan P. 1999. Glucansucrases: Mechanism of action and structure-function relationships. FEMS Microbiol Rev. 23: 131-151.
17. Monsan P, Ouarné F. 2010. Oligosaccharides derived from sucrose. In: Chalarampopoulos D, Rastall RA, editors. Prebiotics and Probiotics Science and Technology. Springer. p. 293-336.
18. Monsan P, Remaud-Siméon M, André I. 2010. Transglucosidases as efficient tools for oligosaccharide and glucoconjugate synthesis. Curr Opin Microbiol. 13: 293-300.
19. Moulis C, Joucla G, Harrison D, Fabre E, Potocki-Veronese G, Monsan P, Remaud-Siméon M. 2006. Understanding the polymerization mechanism of glycoside-hydrolase family 70 glucansucrases. J Biol Chem. 281: 31254-31267.
20. Pijning T, Vujicic-Zagar A, Kralj S, Dijkhuizen L, Dijkstra BW. 2012. Structure of the α-1, 6/α-1, 4-specific glucansucrase GTFA from Lactobacillus reuteri 121. Acta Cryst. F68: 1448-1454.
21. Timmermans JW, de Waard P, Tournois K, Leeflang BR, Vliegenthart JFG. 1993. NMR spectroscopy of nystose. Carbohydr Res. 243: 379-384.
22. Van Geel-Schutten GH, Faber EJ, Smit E, Bonting K, Smith MR, ten Brink B, Kamerling JP, Vliegenthart JFG, Dijkhuizen L. 1999. Biochemical and structural characterization of the glucan and fructan exopolysaccharides synthesized by the Lactobacillus reuteri wild-type strain and by mutant strains. Appl Environ Microbiol. 65: 3008-3014.
23. Van Geel-Schutten GH, Flesch F, ten Brink B, Smith MR, Dijkhuizen L. 1998. Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides. Appl Microbiol Biotechnol. 50: 697-703.
24. Van Leeuwen SS, Kralj S, van Geel-Schutten IH, Gerwig GJ, Dijkhuizen L, Kamerling JP. 2008. Structural analysis of the α-D-glucan (EPS35-5) produced by the Lactobacillus reuteri strain 35-5 glucansucrase GTFA enzyme. Carbohydr Res. 343: 1251-1265.
25. 1H NMR structural-reporter-group concept for the primary structural characterisation of α-D-glucans. Carbohydr Res. 343: 1114-1119.