Molecular characterization of a novel glucosyltransferase from Lactobacillus reuteri strain 121 synthesizing a unique, highly branched glucan with α(1→4) and α(1→6) glucosidic bonds

Applied and Environmental Microbiology

Volumn 68, Issue 9, 2002, Pages 4283-4291

  Kralj, S ^a   Van Geel Schutten, G H ^a   Rahaoui, H ^a   Leer, R J ^a   Faber, E J ^a   Van der Maarel, M J E C ^a   Dijkhuizen, L ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CARBOXYLIC ACIDS; CHEMICAL BONDS; ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; GLUCOSE; MOLECULAR BIOLOGY;

LINKAGES; MOLECULAR CHARACTERIZATION;

ENZYMES;

GLUCAN; GLUCOSYLTRANSFERASE;

BACTERIUM; ENZYME;

AMINO ACID SEQUENCE; ARTICLE; CARBOHYDRATE SYNTHESIS; ENZYME ISOLATION; ENZYME MECHANISM; LACTOBACILLUS REUTERI; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME;

AMINO ACID SEQUENCE; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GLUCANS; GLUCOSYLTRANSFERASES; LACTOBACILLUS; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTOBACILLUS; LACTOBACILLUS REUTERI; POSIBACTERIA; PROKARYOTA;

EID: 0036731751     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.9.4283-4291.2002     Document Type: Article

References (47)

1. Basic local alignment search tool
2. Simple and rapid method for isolating large plasmids DNA from lactic streptococci
3. Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355
4. A rho-recognition site on phage lambda cro-gene mRNA
5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
6. Technique for isolating plasmids from exopolysaccharides-producing Lactobacillus spp.
7. Salt tolerance of lactose-grown Vibrio parahaemolyticus carrying Escherichia coli lac genes
8. A medium for the cultivation of lactobacilli
9. Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharca and characterization of the recombinant enzyme
10. pEMBL: A new family of single stranded plasmids
11. Knowledge-based model of a glucosyltransferase from the oral bacterial group of mutans streptococci
12. Temperature-sensitive dextran-sucrase production by a lactobacillus
13. Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28
14. Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans
15. Water-soluble and water-insoluble glucans produced by Escherichia coli recombinant dextransucrases from Leuconostoc mesenteroides NRRL B-512F
16. Quantification of the structural features of starch polysaccharides by NMR spectroscopy
17. Sequence of the gtfK gene of Streptococcus salivarius ATCC 25975 and evolution of the gtf genes of oral streptococci
18. Definition of a fundamental repeating unit in streptococcal glucosyltransferase glucan-binding regions and related sequences
19. Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans
20. Dextran production by a human oral strain of Lactobacillus casei
21. Studies on transformation of Escherichia coli with plasmids
22. CLUSTAL: A package for performing multiple sequence alignment on a microcomputer
23. Location of repeat elements in glucansucrases of Leuconostoc and Streptococcus species
24. Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products
25. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose
26. A circularly permuted alpha-amylase-type alpha/beta-barrel structure in glucan-synthesizing glucosyltransferases
27. Isolation of an active catalytic core of Streptococcus downei MFe28 GTF-I glucosyltransferase
28. Characterization of Leuconostoc mesenteroides NRRL B-512F dextransucrase (DSRS) and identification of amino-acid residues playing a key role in enzyme activity
29. Glucansucrases: Mechanism of action and structure-function relationships
30. Cloning and sequencing of a gene coding for a novel dextransucrase from Leuconostoc mesenteroides NRRL B-1299 synthesizing only α(1-6) and α(1-3) linkages
31. Degradation of the thiocarbamate herbicide EPTC (S-ethyl dipropylcarbamothioate) and biosafening by Rhodococcus sp. strain N186/21 involve an inducible cytochrome P-450 system and aldehyde dehydrogenase
32. High resolution two-dimensional electrophoresis of proteins
33. Genetics of lactobacilli: Plasmids and gene expression
34. DNA sequencing with chain-terminating inhibitors
35. Dextrans
36. Streptococcus salivarius ATCC 25975 possesses at least two genes coding for primer-independent glucosyltransferases
37. A mutant strain of Leuconostoc mesenteroides B-1355 producing a glucosyltransferase synthesizing 32 α(1→2) glucosidic linkages
38. Sequence analysis of the gtfC gene from Streptococcus mutans GS-5
39. X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family
40. TREECON for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment
41. Engineering of cyclodextrin glycosyltransferase reaction and product specificity
42. Biochemical and structural characterization of the glucan and fructan exopolysaccharides synthesized by the Lactobacillus reuteri wild-type strain and by mutant strains
43. Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides
44. A family of clostridial and streptococcal ligand-binding proteins with conserved C-terminal repeat sequences