CD47 fusion protein targets CD172a+ cells in Crohn's disease and dampens the production of IL-1β and TNF

Journal of Experimental Medicine

Volumn 210, Issue 6, 2013, Pages 1251-1263

  Baba, Nobuyasu ^a   Van, Vu Quang ^a   Wakahara, Keiko ^a   Rubio, Manuel ^a   Fortin, Geneviève ^a   Panzini, Benoît ^a   Soucy, Geneviève ^a   Wassef, Ramses ^a   Richard, Carole ^a   Tamaz, Raja ^a   Lahaie, Raymond ^a   Bernard, Edmond Jean ^a   Caussignac, Yves ^a   Leduc, Raymond ^a   Lougnarath, Rasmy ^a   Bergeron, Carole ^a   Racicot, Marc André ^a   Bergeron, Fanny ^a   Panzini, Marie Andrée ^a   Demetter, Pieter ^a   Franchimont, Denis ^b   Schäkel, Knut ^c   Weckbecker, Gisbert ^d   Kolbinger, Frank ^d   Heusser, Christoph ^d   Huber, Thomas ^d   Welzenbach, Karl ^d   Sarfati, Marika ^a   more..

^a CENTRE HOSPITALIER DE L UNIVERSITÉ DE MONTRÉAL   (Canada)

^b ERASME HOSPITAL   (Belgium)

^c UNIVERSITY OF HEIDELBERG   (Germany)

^d NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CD172A ANTIGEN; CD172B ANTIGEN; CD209 ANTIGEN; CD47 ANTIGEN; CHEMOKINE RECEPTOR CX3CR1; GAMMA INTERFERON; GLYCOPROTEIN P 15095; HLA DR ANTIGEN; HYBRID PROTEIN; INTERLEUKIN 1BETA; INTERLEUKIN 23; INTERLEUKIN 6; INTERLEUKIN 8; LYMPHOCYTE ANTIGEN; MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA; MACROPHAGE INFLAMMATORY PROTEIN 1BETA; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG; UVOMORULIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; COLITIS; CONTROLLED STUDY; CROHN DISEASE; CYTOKINE PRODUCTION; DENDRITIC CELL; ENTERITIS; HUMAN; HUMAN TISSUE; INFLAMMATORY CELL; INTESTINE MUCOSA; MESENTERY LYMPH NODE; MONONUCLEAR PHAGOCYTE; MOUSE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; T LYMPHOCYTE;

ANTIGENS, CD47; ANTIGENS, DIFFERENTIATION; CADHERINS; CROHN DISEASE; DENDRITIC CELLS; HLA-DR ANTIGENS; HUMANS; INFLAMMATION; INTERLEUKIN-1BETA; INTESTINAL MUCOSA; LYMPH NODES; MONOCYTES; RECEPTORS, CHEMOKINE; RECEPTORS, IMMUNOLOGIC; RECOMBINANT FUSION PROTEINS; TH1 CELLS; TH17 CELLS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84880649447     PISSN: 00221007     EISSN: 15409538     Source Type: Journal    
DOI: 10.1084/jem.20122037     Document Type: Article

References (63)

1. Acosta-Rodriguez, E.V., G. Napolitani, A. Lanzavecchia, and F. Sallusto. 2007. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol. 8:942-949. http://dx.doi.org/10.1038/ni1496
2. Autschbach, F., J. Braunstein, B. Helmke, I. Zuna, G. Schürmann, Z.I. Niemir, R. Wallich, H.F. Otto, and S.C. Meuer. 1998. In situ expression of interleukin-10 in noninflamed human gut and in inflammatory bowel disease. Am. J. Pathol. 153:121-130. http://dx.doi.org/10.1016/S0002-9440(10)65552-6
3. Baba, N., S. Samson, R. Bourdet-Sicard, M. Rubio, and M. Sarfati. 2008. Commensal bacteria trigger a full dendritic cell maturation program that promotes the expansion of non-Tr1 suppressor T cells. J. Leukoc. Biol. 84:468-476. http://dx.doi.org/10.1189/jlb.0108017
4. Bain, C.C., C.L. Scott, H. Uronen-Hansson, S. Gudjonsson, O. Jansson, O. Grip, M. Guilliams, B. Malissen, W.W. Agace, and A.M. Mowat. 2013. Resident and pro-inflammatory macrophages in the colon represent alternative context-dependent fates of the same Ly6C(hi) monocyte precursors. Mucosal Immunol. 6:498-510. http://dx.doi.org/10.1038/mi.2012.89
5. Bar-On, L., E. Zigmond, and S. Jung. 2011. Management of gut inflammation through the manipulation of intestinal dendritic cells and macrophages? Semin. Immunol. 23:58-64. http://dx.doi.org/10.1016/j.smim.2011.01.002
6. Birnberg, T., L. Bar-On, A. Sapoznikov, M.L. Caton, L. Cervantes-Barragán, D. Makia, R. Krauthgamer, O. Brenner, B. Ludewig, D. Brockschnieder, et al. 2008. Lack of conventional dendritic cells is compatible with normal development and T cell homeostasis, but causes myeloid proliferative syndrome. Immunity. 29:986-997. http://dx.doi.org/10.1016/j.immuni.2008.10.012
7. Bogunovic, M., F. Ginhoux, J. Helft, L. Shang, D. Hashimoto, M. Greter, K. Liu, C. Jakubzick, M.A. Ingersoll, M. Leboeuf, et al. 2009. Origin of the lamina propria dendritic cell network. Immunity. 31:513-525. http://dx.doi.org/10.1016/j.immuni.2009.08.010
8. Braun, D., L. Galibert, T. Nakajima, H. Saito, V.V. Quang, M. Rubio, and M. Sarfati. 2006. Semimature stage: a checkpoint in a dendritic cell maturation program that allows for functional reversion after signal-regulatory protein-alpha ligation and maturation signals. J. Immunol. 177:8550-8559.
9. Brown, E.J., and W.A. Frazier. 2001. Integrin-associated protein (CD47) and its ligands. Trends Cell Biol. 11:130-135. http://dx.doi.org/10.1016/S0962-8924(00)01906-1
10. Bull, D.M., and M.A. Bookman. 9077. Isolation and functional characterization of human intestinal mucosal lymphoid cells. J. Clin. Invest. 59:966-974.
11. Cerovic, V., S.A. Houston, C.L. Scott, A. Aumeunier, U. Yrlid, A.M. Mowat, and S.W. Milling. 2013. Intestinal CD103(-) dendritic cells migrate in lymph and prime effector T cells. Mucosal Immunol. 6:104-113. http://dx.doi.org/10.1038/mi.2012.53
12. Chassaing, B., and A. Darfeuille-Michaud. 2011. The commensal microbiota and enteropathogens in the pathogenesis of inflammatory bowel diseases. Gastroenterology. 140:1720-1728. http://dx.doi.org/10.1053/j.gastro.2011.01.054
13. Cheong, C., I. Matos, J.H. Choi, D.B. Dandamudi, E. Shrestha, M.P. Longhi, K.L. Jeffrey, R.M. Anthony, C. Kluger, G. Nchinda, et al. 2010. Microbial stimulation fully differentiates monocytes to DC-SIGN/CD209(+) dendritic cells for immune T cell areas. Cell. 143:416-429. http://dx.doi.org/10.1016/j.cell.2010.09.039
14. Coombes, J.L., and F. Powrie. 2008. Dendritic cells in intestinal immune regulation. Nat. Rev. Immunol. 8:435-446. http://dx.doi.org/10.1038/nri2335
15. Cros, J., N. Cagnard, K. Woollard, N. Patey, S.Y. Zhang, B. Senechal, A. Puel, S.K. Biswas, D. Moshous, C. Picard, et al. 2010. Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors. Immunity. 33:375-386. http://dx.doi.org/10.1016/j.immuni.2010.08.012
16. de Baey, A., I. Mende, G. Baretton, A. Greiner, W.H. Hartl, P.A. Baeuerle, and H.M. Diepolder. 2003. A subset of human dendritic cells in the T cell area of mucosa-associated lymphoid tissue with a high potential to produce TNF-alpha. J. Immunol. 170:5089-5094.
17. Denning, T.L., Y.C. Wang, S.R. Patel, I.R. Williams, and B. Pulendran. 2007. Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses. Nat. Immunol. 8:1086-1094. http://dx.doi.org/10.1038/ni1511
18. Diehl, G.E., R.S. Longman, J.X. Zhang, B. Breart, C. Galan, A. Cuesta, S.R. Schwab, and D.R. Littman. 2013. Microbiota restricts trafficking of bacteria to mesenteric lymph nodes by CX(3)CR1(hi) cells. Nature. 494:116-120. http://dx.doi.org/10.1038/nature11809
19. Fortin, G., M. Raymond, V.Q. Van, M. Rubio, P. Gautier, M. Sarfati, and D. Franchimont. 2009. A role for CD47 in the development of experimental colitis mediated by SIRPalpha+CD103-dendritic cells. J. Exp. Med. 206:1995-2011. http://dx.doi.org/10.1084/jem.20082805
20. Gautier, E.L., T. Shay, J. Miller, M. Greter, C. Jakubzick, S. Ivanov, J. Helft, A. Chow, K.G. Elpek, S. Gordonov, et al; Immunological Genome Consortium. 2012. Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages. Nat. Immunol. 13:1118-1128. http://dx.doi.org/10.1038/ni.2419
21. Ginhoux, F., K. Liu, J. Helft, M. Bogunovic, M. Greter, D. Hashimoto, J. Price, N. Yin, J. Bromberg, S.A. Lira, et al. 2009. The origin and development of nonlymphoid tissue CD103+ DCs. J. Exp. Med. 206:3115-3130. http://dx.doi.org/10.1084/jem.20091756
22. Grimm, M.C., P. Pavli, E. Van de Pol, and W.F. Doe. 9095a. Evidence for a CD14+ population of monocytes in inflammatory bowel disease mucosa-implications for pathogenesis. Clin. Exp. Immunol. 100:291-297. http://dx.doi.org/10.1111/j.1365-2249.1995.tb03667.x
23. Grimm, M.C., W.E. Pullman, G.M. Bennett, P.J. Sullivan, P. Pavli, and W.F. Doe. 9095b. Direct evidence of monocyte recruitment to inflammatory bowel disease mucosa. J. Gastroenterol. Hepatol. 10:387-395. http://dx.doi.org/10.1111/j.1440-1746.1995.tb01589.x
24. Herfarth, H., and J. Schölmerich. 2002. IL-10 therapy in Crohn's disease: at the crossroads. Treatment of Crohn's disease with the anti-inflammatory cytokine interleukin 10. Gut. 50:146-147. http://dx.doi.org/10.1136/gut.50.2.146
25. Jaensson, E., H. Uronen-Hansson, O. Pabst, B. Eksteen, J. Tian, J.L. Coombes, P.L. Berg, T. Davidsson, F. Powrie, B. Johansson-Lindbom, and W.W. Agace. 2008. Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J. Exp. Med. 205:2139-2149. http://dx.doi.org/10.1084/jem.20080414
26. Kamada, N., T. Hisamatsu, S. Okamoto, H. Chinen, T. Kobayashi, T. Sato, A. Sakuraba, M.T. Kitazume, A. Sugita, K. Koganei, et al. 2008. Unique CD14 intestinal macrophages contribute to the pathogenesis of Crohn disease via IL-23/IFN-gamma axis. J. Clin. Invest. 118:2269-2280.
27. Kamada, N., T. Hisamatsu, H. Honda, T. Kobayashi, H. Chinen, M.T. Kitazume, T. Takayama, S. Okamoto, K. Koganei, A. Sugita, et al. 2009. Human CD14+ macrophages in intestinal lamina propria exhibit potent antigen-presenting ability. J. Immunol. 183:1724-1731. http://dx.doi.org/10.4049/jimmunol.0804369
28. Kaser, A., S. Zeissig, and R.S. Blumberg. 2010. Inflammatory bowel disease. Annu. Rev. Immunol. 28:573-621. http://dx.doi.org/10.1146/annurev-immunol-030409-101225
29. Kobayashi, T., S. Okamoto, T. Hisamatsu, N. Kamada, H. Chinen, R. Saito, M.T. Kitazume, A. Nakazawa, A. Sugita, K. Koganei, et al. 2008. IL23 differentially regulates the Th1/Th17 balance in ulcerative colitis and Crohn's disease. Gut. 57:1682-1689. http://dx.doi.org/10.1136/gut.2007.135053
30. Kryczek, I., E. Zhao, Y. Liu, Y. Wang, L. Vatan, W. Szeliga, J. Moyer, A. Klimczak, A. Lange, and W. Zou. 2011. Human TH17 cells are longlived effector memory cells. Sci. Transl. Med. 3:104ra100. http://dx.doi.org/10.1126/scitranslmed.3002949
31. Latour, S., H. Tanaka, C. Demeure, V. Mateo, M. Rubio, E.J. Brown, C. Maliszewski, F.P. Lindberg, A. Oldenborg, A. Ullrich, et al. 2001. Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation. J. Immunol. 167:2547-2554.
32. Liu, K., G.D. Victora, T.A. Schwickert, P. Guermonprez, M.M. Meredith, K. Yao, F.F. Chu, G.J. Randolph, A.Y. Rudensky, and M. Nussenzweig. 2009. In vivo analysis of dendritic cell development and homeostasis. Science. 324:392-397. http://dx.doi.org/10.1126/science.1171243
33. MacDonald, T.T., I. Monteleone, M.C. Fantini, and G. Monteleone. 2011. Regulation of homeostasis and inflammation in the intestine. Gastroenterology. 140:1768-1775. http://dx.doi.org/10.1053/j.gastro.2011.02.047
34. Maeda, S., L.C. Hsu, H. Liu, L.A. Bankston, M. Iimura, M.F. Kagnoff, L. Eckmann, and M. Karin. 2005. Nod2 mutation in Crohn's disease potentiates NF-kappaB activity and IL-1beta processing. Science. 307:734-738. http://dx.doi.org/10.1126/science.1103685
35. Maloy, K.J., and F. Powrie. 2011. Intestinal homeostasis and its breakdown in inflammatory bowel disease. Nature. 474:298-306. http://dx.doi.org/10.1038/nature10208
36. Medina-Contreras, O., D. Geem, O. Laur, I.R. Williams, S.A. Lira, A. Nusrat, C.A. Parkos, and T.L. Denning. 2011. CX3CR1 regulates intestinal macrophage homeostasis, bacterial translocation, and colitogenic Th17 responses in mice. J. Clin. Invest. 121:4787-4795. http://dx.doi.org/10.1172/JCI59150
37. Miller, J.C., B.D. Brown, T. Shay, E.L. Gautier, V. Jojic, A. Cohain, G. Pandey, M. Leboeuf, K.G. Elpek, J. Helft, et al; Immunological Genome Consortium. 2012. Deciphering the transcriptional network of the dendritic cell lineage. Nat. Immunol. 13:888-899. http://dx.doi.org/10.1038/ni.2370
38. Mills, K.H., and A. Dunne. 2009. Immune modulation: IL-1, master mediator or initiator of inflammation. Nat. Med. 15:1363-1364. http://dx.doi.org/10.1038/nm1209-1363
39. Plantinga, M., M. Guilliams, M. Vanheerswynghels, K. Deswarte, F. Branco-Madeira, W. Toussaint, L. Vanhoutte, K. Neyt, N. Killeen, B. Malissen, et al. 2013. Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain T helper 2 cell-mediated immunity to house dust mite allergen. Immunity. 38:322-335. http://dx.doi.org/10.1016/j.immuni.2012.10.016
40. Raymond, M., M. Rubio, G. Fortin, K.H. Shalaby, H. Hammad, B.N. Lambrecht, and M. Sarfati. 2009. Selective control of SIRP-alphapositive airway dendritic cell trafficking through CD47 is critical for the development of T(H)2-mediated allergic inflammation. J. Allergy Clin. Immunol. 124:1333-1342: e1. http://dx.doi.org/10.1016/j.jaci.2009.07.021
41. Rivollier, A., J. He, A. Kole, V. Valatas, and B.L. Kelsall. 2012. Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon. J. Exp. Med. 209:139-155. http://dx.doi.org/10.1084/jem.20101387
42. Schäkel, K., M. von Kietzell, A. Hänsel, A. Ebling, L. Schulze, M. Haase, C. Semmler, M. Sarfati, A.N. Barclay, G.J. Randolph, et al. 2006. Human 6-sulfo LacNAc-expressing dendritic cells are principal producers of early interleukin-12 and are controlled by erythrocytes. Immunity. 24:767-777. http://dx.doi.org/10.1016/j.immuni.2006.03.020
43. Schenk, M., A. Bouchon, F. Seibold, and C. Mueller. 2007. TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases. J. Clin. Invest. 117:3097-3106. http://dx.doi.org/10.1172/JCI30602
44. Schulthess, J., B. Meresse, E. Ramiro-Puig, N. Montcuquet, S. Darche, B. Bègue, F. Ruemmele, C. Combadière, J.P. Di Santo, D. Buzoni-Gatel, and N. Cerf-Bensussan. 2012. Interleukin-15-dependent NKp46+ innate lymphoid cells control intestinal inflammation by recruiting inflammatory monocytes. Immunity. 37:108-121. http://dx.doi.org/10.1016/j.immuni.2012.05.013
45. Schulz, O., E. Jaensson, E.K. Persson, X. Liu, T. Worbs, W.W. Agace, and O. Pabst. 2009. Intestinal CD103+, but not CX3CR1+, antigen sampling cells migrate in lymph and serve classical dendritic cell functions. J. Exp. Med. 206:3101-3114. http://dx.doi.org/10.1084/jem.20091925
46. Segura, E., J. Valladeau-Guilemond, M.H. Donnadieu, X. Sastre-Garau, V. Soumelis, and S. Amigorena. 2012. Characterization of resident and migratory dendritic cells in human lymph nodes. J. Exp. Med. 209:653-660. http://dx.doi.org/10.1084/jem.20111457
47. Segura, E., M. Touzot, A. Bohineust, A. Cappuccio, G. Chiocchia, A. Hosmalin, M. Dalod, V. Soumelis, and S. Amigorena. 2013. Human inflammatory dendritic cells induce Th17 cell differentiation. Immunity. 38:336-348. http://dx.doi.org/10.1016/j.immuni.2012.10.018
48. Seiffert, M., P. Brossart, C. Cant, M. Cella, M. Colonna, W. Brugger, L. Kanz, A. Ullrich, and H.J. Bühring. 2001. Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. Blood. 97:2741-2749. http://dx.doi.org/10.1182/blood. V97.9.2741
49. Siddiqui, K.R., S. Laffont, and F. Powrie. 2010. E-cadherin marks a subset of inflammatory dendritic cells that promote T cell-mediated colitis. Immunity. 32:557-567. http://dx.doi.org/10.1016/j.immuni.2010.03.017
50. Smith, P.D., L.E. Smythies, R. Shen, T. Greenwell-Wild, M. Gliozzi, and S.M. Wahl. 2011. Intestinal macrophages and response to microbial encroachment. Mucosal Immunol. 4:31-42. http://dx.doi.org/10.1038/mi.2010.66
51. Smythies, L.E., M. Sellers, R.H. Clements, M. Mosteller-Barnum, G. Meng, W.H. Benjamin, J.M. Orenstein, and P.D. Smith. 2005. Human intestinal macrophages display profound inflammatory anergy despite avid phagocytic and bacteriocidal activity. J. Clin. Invest. 115:66-75.
52. Sun, C.M., J.A. Hall, R.B. Blank, N. Bouladoux, M. Oukka, J.R. Mora, and Y. Belkaid. 2007. Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid. J. Exp. Med. 204:1775-1785. http://dx.doi.org/10.1084/jem.20070602
53. Tamoutounour, S., S. Henri, H. Lelouard, B. de Bovis, C. de Haar, C.J. van der Woude, A.M. Woltman, Y. Reyal, D. Bonnet, D. Sichien, et al. 2012. CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis. Eur. J. Immunol. 42:3150-3166. http://dx.doi.org/10.1002/eji.201242847
54. te Velde, A.A., Y. van Kooyk, H. Braat, D.W. Hommes, T.A. Dellemijn, J.F. Slors, S.J. van Deventer, and F.A. Vyth-Dreese. 2003. Increased expression of DC-SIGN+IL-12+IL-18+ and CD83+IL-12-IL-18-dendritic cell populations in the colonic mucosa of patients with Crohn's disease. Eur. J. Immunol. 33:143-151. http://dx.doi.org/10.1002/immu.200390017
55. van den Berg, T.K., and C.E. van der Schoot. 2008. Innate immune 'self' recognition: a role for CD47-SIRPalpha interactions in hematopoietic stem cell transplantation. Trends Immunol. 29:203-206. http://dx.doi.org/10.1016/j.it.2008.02.004
56. Varol, C., A. Vallon-Eberhard, E. Elinav, T. Aychek, Y. Shapira, H. Luche, H.J. Fehling, W.D. Hardt, G. Shakhar, and S. Jung. 2009. Intestinal lamina propria dendritic cell subsets have different origin and functions. Immunity. 31:502-512. http://dx.doi.org/10.1016/j.immuni.2009.06.025
57. Varol, C., E. Zigmond, and S. Jung. 2010. Securing the immune tightrope: mononuclear phagocytes in the intestinal lamina propria. Nat. Rev. Immunol. 10:415-426. http://dx.doi.org/10.1038/nri2778
58. Verstege, M.I., F.J. ten Kate, S.M. Reinartz, C.M. van Drunen, F.J. Slors, W.A. Bemelman, F.A. Vyth-Dreese, and A.A. te Velde. 2008. Dendritic cell populations in colon and mesenteric lymph nodes of patients with Crohn's disease. J. Histochem. Cytochem. 56:233-241. http://dx.doi.org/10.1369/jhc.7A7308.2007
59. Villani, A.C., M. Lemire, G. Fortin, E. Louis, M.S. Silverberg, C. Collette, N. Baba, C. Libioulle, J. Belaiche, A. Bitton, et al. 2009. Common variants in the NLRP3 region contribute to Crohn's disease susceptibility. Nat. Genet. 41:71-76. http://dx.doi.org/10.1038/ng.285
60. Vos, A.C., M.E. Wildenberg, I. Arijs, M. Duijvestein, A.P. Verhaar, G. de Hertogh, S. Vermeire, P. Rutgeerts, G.R. van den Brink, and D.W. Hommes. 2012. Regulatory macrophages induced by infliximab are involved in healing in vivo and in vitro. Inflamm. Bowel Dis. 18:401-408. http://dx.doi.org/10.1002/ibd.21818
61. Weber, B., L. Saurer, M. Schenk, N. Dickgreber, and C. Mueller. 2011. CX3CR1 defines functionally distinct intestinal mononuclear phagocyte subsets which maintain their respective functions during homeostatic and inflammatory conditions. Eur. J. Immunol. 41:773-779. http://dx.doi.org/10.1002/eji.201040965
62. Zhao, X.W., E.M. van Beek, K. Schornagel, H. Van der Maaden, M. Van Houdt, M.A. Otten, P. Finetti, M. Van Egmond, T. Matozaki, G. Kraal, et al. 2011. CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction. Proc. Natl. Acad. Sci. USA. 108:18342-18347. http://dx.doi.org/10.1073/pnas.1106550108
63. Zielinski, C.E., F. Mele, D. Aschenbrenner, D. Jarrossay, F. Ronchi, M. Gattorno, S. Monticelli, A. Lanzavecchia, and F. Sallusto. 2012. Pathogen-induced human TH17 cells produce IFN-? or IL-10 and are regulated by IL-1β. Nature. 484:514-518. http://dx.doi.org/10.1038/nature10957