EtBr-induced selective degradation of mitochondria occurs via autophagy

Oncology Reports

Volumn 30, Issue 3, 2013, Pages 1201-1208

  Luo, Yan ^a   Hu, Yide ^a   Zhang, Minghui ^a   Xiao, Yu ^a   Song, Zongchang ^a   Xu, Yu ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Autophagy; Beclin 1; Ethidium bromide; Lung cancer cells; Mitochondria

Indexed keywords

BECLIN 1; ETHIDIUM BROMIDE; MICROTUBULE ASSOCIATED PROTEIN; MICROTUBULE ASSOCIATED PROTEIN LIGHT CHAIN 3; PHOSPHATIDYLINOSITOL 3 KINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTOPHAGY; CANCER CELL CULTURE; CELL MIGRATION; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; GROWTH RATE; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; LUNG CANCER; MITOPHAGY; MOUSE; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; TUMOR VOLUME;

ANIMALS; APOPTOSIS REGULATORY PROTEINS; AUTOPHAGY; BLOTTING, WESTERN; CELL MOVEMENT; CELL PROLIFERATION; ETHIDIUM; FLUORESCENT DYES; HUMANS; LUNG NEOPLASMS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MEMBRANE PROTEINS; MICE; MICE, INBRED NOD; MICE, SCID; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; MITOCHONDRIA; PHOSPHATIDYLINOSITOL 3-KINASES; SIGNAL TRANSDUCTION; TUMOR CELLS, CULTURED; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 84880611302     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2013.2590     Document Type: Article

References (35)

1. Mizushima N, Levine B, Cuervo AM and Klionsky DJ: Autophagy fights disease through cellular self-digestion. Nature 451: 1069-1075, 2008.
2. Eskelinen EL and Saftig P: Autophagy: a lysosomal degradation pathway with a central role in health and disease. Biochim Biophys Acta 1793: 664-673, 2009.
3. Huang J and Klionsky DJ: Autophagy and human disease. Cell Cycle 6: 1837-1849, 2007.
4. Yue Z, Jin S, Yang C, Levine AJ and Heintz N: Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc Natl Acad Sci USA 100: 15077-15082, 2003.
5. Jin S: Autophagy, mitochondrial quality control, and oncogenesis. Autophagy 2: 80-84, 2006.
6. Mizushima N, Yoshimori T and Ohsumi Y: The role of Atg proteins in autophagosome formation. Annu Rev Cell Dev Biol 27: 107-132, 2011.
7. Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y and Yoshimori T: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J 19: 5720-5728, 2000.
8. Malhotra JD and Kaufman RJ: ER stress and its functional link to mitochondria: role in cell survival and death. Cold Spring Harb Perspect Biol 3: a004424, 2011.
9. Gupta KJ, Igamberdiev AU and Mur LA: NO and ROS homeostasis in mitochondria: a central role for alternative oxidase. New Phytol 195: 1-3, 2012.
10. Lee J, Giordano S and Zhang J: Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling. Biochem J 441: 523-540, 2012.
11. Rambold AS and Lippincott-Schwartz J: Mechanisms of mitochondria and autophagy crosstalk. Cell Cycle 10: 4032-4038, 2011.
12. Bhatia-Kiššová I and Camougrand N: Mitophagy: a process that adapts to the cell physiology. Int J Biochem Cell Biol 45: 30-33, 2013.
13. Ashrafi G and Schwarz TL: The pathways of mitophagy for quality control and clearance of mitochondria. Cell Death Differ 20: 31-42, 2013.
14. Hashiguchi K and Zhang-Akiyama QM: Establishment of human cell lines lacking mitochondrial DNA. Methods Mol Biol 554: 383-391, 2009.
15. Kang R, Zeh HJ, Lotze MT and Tang D: The Beclin 1 network regulates autophagy and apoptosis. Cell Death Differ 18: 571-580, 2011.
16. Alexeyev MF: Is there more to aging than mitochondrial DNA and reactive oxygen species? FEBS J 276: 5768-5787, 2009.
17. Garcia N, Hernández-Esquivel L, Zazueta C, Martínez-Abundis E, Pavón N and Chávez E: Induction of mitochondrial permeability transition by the DNA-intercalating cationic dye ethidium bromide. J Biochem 146: 887-894, 2009.
18. Armand R, Channon JY, Kintner J, White KA, Miselis KA, Perez RP and Lewis LD: The effects of ethidium bromide induced loss of mitochondrial DNA on mitochondrial phenotype and ultrastructure in a human leukemia T-cell line (MOLT-4 cells). Toxicol Appl Pharmacol 196: 68-79, 2004.
19. von Wurmb-Schwark N, Cavelier L and Cortopassi GA: A low dose of ethidium bromide leads to an increase of total mitochondrial DNA while higher concentrations induce the mtDNA 4997 deletion in a human neuronal cell line. Mutat Res 596: 57-63, 2006.
20. Chazotte B: Labeling mitochondria with TMRM or TMRE. Cold Spring Harb Protoc 2011: 895-897, 2011.
21. Rodriguez-Enriquez S, Kim I, Currin RT and Lemasters JJ: Tracker dyes to probe mitochondrial autophagy (mitophagy) in rat hepatocytes. Autophagy 2: 39-46, 2006.
22. Kim I and Lemasters JJ: Mitochondrial degradation by autophagy (mitophagy) in GFP-LC3 transgenic hepatocytes during nutrient deprivation. Am J Physiol Cell Physiol 300: C308-C317, 2011.
23. Dewey WC and Fuhr MA: Quantification of mitochondria during the cell cycle of Chinese hamster cells. Exp Cell Res 99: 23-30, 1976.
24. Segui-Simarro JM, Coronado MJ and Staehelin LA: The mitochondrial cycle of Arabidopsis shoot apical meristem and leaf primordium meristematic cells is defined by a perinuclear tentaculate/cage-like mitochondrion. Plant Physiol 148: 1380-1393, 2008.
25. Mizushima N, Yoshimori T and Levine B: Methods in mammalian autophagy research. Cell 140: 313-326, 2010.
26. Wu YT, Tan HL, Shui G, Bauvy C, Huang Q, Wenk MR, Ong CN, Codogno P and Shen HM: Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. J Biol Chem 285: 10850-10861, 2010.
27. Petiot A, Ogier-Denis E, Blommaart EF, Meijer AJ and Codogno P: Distinct classes of phosphatidylinositol 3'-kinases are involved in signaling pathways that control macroautophagy in HT-29 cells. J Biol Chem 275: 992-998, 2000.
28. Chen N and Karantza-Wadsworth V: Role and regulation of autophagy in cancer. Biochim Biophys Acta 1793: 1516-1523, 2009.
29. White E, Karp C, Strohecker AM, Guo Y and Mathew R: Role of autophagy in suppression of inflammation and cancer. Curr Opin Cell Biol 22: 212-217, 2010.
30. Gorski SM, Ries J and Lum JJ: Targeting autophagy: the Achilles' heel of cancer. Autophagy 8: 1279-1280, 2012.
31. Kulawiec M, Ayyasamy V and Singh KK: p53 regulates mtDNA copy number and mitocheckpoint pathway. J Carcinog 8: 8, 2009.
32. Yu M, Shi Y, Wei X, Yang Y, Zhou Y, Hao X, Zhang N and Niu R: Depletion of mitochondrial DNA by ethidium bromide treatment inhibits the proliferation and tumorigenesis of T47D human breast cancer cells. Toxicol Lett 170: 83-93, 2007.
33. Singh KK: Mitochondria damage checkpoint, aging, and cancer. Ann NY Acad Sci 1067: 182-190, 2006.
34. Amuthan G, Biswas G, Ananadatheerthavarada HK, Vijayasarathy C, Shephard HM and Avadhani NG: Mitochondrial stress-induced calcium signaling, phenotypic changes and invasive behavior in human lung carcinoma A549 cells. Oncogene 21: 7839-7849, 2002.
35. Chen D, Xue W and Xiang J: The intra-nucleus integration of mitochondrial DNA (mtDNA) in cervical mucosa cells and its relation with c-myc expression. J Exp Clin Cancer Res 27: 36, 2008.