Isolation of mutants of Acinetobacter calcoaceticus deficient in wax ester synthesis and complementation of one mutation with a gene encoding a fatty acyl coenzyme A reductase

Journal of Bacteriology

Volumn 179, Issue 9, 1997, Pages 2969-2975

  Reiser, Steven ^a,b   Somerville, Chris ^a  

^a GEOPHYSICAL LABORATORY   (United States)

^b MONSANTO COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL COENZYME A DEHYDROGENASE; ESTER; HEXADECANE; HEXADECANOL; NITROGEN; NITROSOGUANIDINE; PALMITIC ACID; WAX;

ACINETOBACTER CALCOACETICUS; ARTICLE; BACTERIUM MUTANT; CATALYSIS; ESCHERICHIA COLI; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; REDUCTION;

EID: 0031002077     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.9.2969-2975.1997     Document Type: Article

References (27)

1. Allen, L. N., and R. S. Hanson. 1985. Construction of broad-host-range cosmid cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol. J. Bacteriol. 161:955-962.
2. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
3. Arrowsmith, T. J., F. Malpartida, D. H. Sherman, A. Birch, D. A. Hopwood, and J. A. Robinson. 1992. Characterization of act1-homologous DNA encoding polyketide synthase genes from the monensin producer Streptomyces cinnamonensis. Mol. Gen. Genet. 234:254-264.
4. Asperger, O., and H.-P. Kleber. Metabolism of alkanes by Acinetobacter, p. 323-350. In K. J. Towner, E. Bergogne-Bérézin, and C. A. Fewson (ed.), The biology of Acinetobacter: taxonomy, clinical importance, molecular biology, physiology, industrial relevance. FEMS Symposium No. 57. New York. N. Y.
5. Bachmann, B. J. 1987. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12, p. 1190-1219. In F. C. Neidhardt. J. L. Ingraham. K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
6. 20, n-alkanes. J. Am. Oil Chem. Soc. 59:69-74.
7. Ervin, J. L., J. Geigert, S. L. Neidleman, and J. Wadsworth. 1984. Substratedependent and growth temperature-dependent changes in the wax ester compositions produced by Acinetobacter sp. H01-N, p. 217-222. in C. Ratledge, P. Dawson. and L. Rattray (ed.), Biotechnology for the oils and fats industry. American Oil Chemists Society. Champaign, III.
8. Figurski, D., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
9. Finnerty, W. R. 1989. Lipids of Acinetobacter, p. 184-188. In T. H. Applewhite, (ed.). Proceedings of the World Conference on Biotechnology for the Fats and Oils Industry. American Oil Chemists Society, Champaign. Ill.
10. Fixter, L. M., and M. K. Sherwani. 1991. Energy reserves in Acinetobacter, p. 273-294. In K. J. Towner, E. Bergogne-Bérézin. and C. A. Fewson (ed.). The biology of Acinetobacter: taxonomy, clinical importance, molecular biology, physiology, industrial relevance. FEMS Symposium No. 57. New York. N.Y.
11. Fox, M. G. A., M. Dickinson, and C. Ratledge. 1992. Long-chain alcohol and aldehyde dehydrogenase activities in Acinetobacter calcoaceticus strain H01-N. J. Gen. Microbiol. 138:1963-1972.
12. Juni, E., and A. Janik. 1969. Transformation of Acinetobacter calcoaceticus (Bacterium anitratum). J. Bacteriol. 98:281-288.
13. Kleckner, N., J. Bender, and S. Gottesman. 1991. Uses of transposons with emphasis on Tn10, p. 139-180. In J. H. Miller (ed.). Methods in enzymology: bacterial genetic systems, vol. 204. Academic Press Inc., San Diego, Calif.
14. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
15. Makula, R. A., P. J. Lockwood, and W. R. Finnerty. 1975. Comparative analysis of lipids of Acinetobacter species grown on hexadecane. J. Bacteriol. 121:250-258.
16. Metz, J. G., K. D. Lardizabal, and M. W. Lassner. August 1995. U.S. patent 5,445.947.
17. Persson, B., M. Kroo, and H. Jörnvall. 1991. Characteristics of short-chain alcohol dehydrogenases and related enzymes. Eur. J. Biochem. 200:537-543.
18. Pollard, M. R., and J. G. Metz. May 1995. U.S. patent 5,411,879.
19. Raleigh, E. A., K. Lech, and R. Brent. 1989. Selected topics from classical bacterial genetics, p. 1.4.1-1.4.14. In F. M. Ausubel (ed.), Current protocols in molecular biology. Publishing Associates and Wiley Ir erscience, New York, N.Y.
20. Reiser, S. E. 1996. Genetic analysis of wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus strain BD413. Ph.D. thesis. Michigan State University, East Lansing.
21. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N.Y.
22. Scott, C. C. L., and W. R. Finnerty. 1976. Characterization of intracytoplasmic hydrocarbon inclusions from the hydrocarbon-oxidizing Acinetobacter species H01-N. J. Bacteriol. 127:481-489.
23. Singer, M. E., S. M. Tyler, and W. R. Finnerty. 1985. Growth of Acinetobacter sp. strain H01-N on n-hexadecanol: physiological and ultrastructural characteristics. Mol. Gen. Genet. 162:162-169.
24. Singer, M. E., and W. R. Finnerty. 1985. Alcohol dehydrogenases in Acinetobacter sp. strain H01-N: role in hexadecane and hexadecanol metabolism. J. Bacteriol. 164:1017-1024.
25. Singer, M. E., and W. R. Finnerty. 1985. Fatty aldehyde dehydrogenases in Acinetobacter sp. strain H01-N: role in hexadecane and hexadecanol metabolism. J. Bacteriol. 164:1011-1016.
26. Wang, L., K. Takayama, D. S. Goldman, and H. K. Schnoes. 1972. Synthesis of alcohol and wax ester by a cell-free system in Mycobacterium tuberculosis. Biochim. Biophys. Acta 260:41-18.
27. Yuan, Y., R. E. Lee, G. S. Besra, J. T. Belisle, and C. E. Barry. 1995. Identification of a gene involved in the biosynthesis of cyclopropanated mycolic acids in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 92:6630-6634.