A high throughput method for enrichment of natural killer cells and lymphocytes and assessment of in vitro cytotoxicity

Journal of Immunological Methods

Volumn 394, Issue 1-2, 2013, Pages 40-48

  So, Edward C ^a,b   Sallin, Michelle A ^b   Zhang, Xiaoyu ^b   Chan, Siaw L ^c   Sahni, Lepakshi ^a,b   Schulze, Dan H ^b   Davila, Eduardo ^b,d   Strome, Scott E ^b   Jain, Ajay ^a,b  

^a BALTIMORE VA MEDICAL CENTER   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c Gliknik Inc   (United States)

^d UNIVERSITY OF MARYLAND GREENEBAUM CANCER CENTER   (United States)

Author keywords

ADCC; Calcein; Chromium release; Magnetic cell separation; Rapid high throughput; Tetrameric antibody complexes

Indexed keywords

CALCEIN; CD94 ANTIGEN; GRANZYME B; NATURAL CYTOTOXICITY TRIGGERING RECEPTOR 1; NATURAL CYTOTOXICITY TRIGGERING RECEPTOR 3; NATURAL KILLER CELL RECEPTOR NKG2D; PERFORIN;

ANALYTICAL EQUIPMENT; ANTIGEN EXPRESSION; ARTICLE; CELL DENSITY; CELL ISOLATION; CELL SELECTION; CELL SEPARATION; CYTOTOXICITY; DENSITY GRADIENT CENTRIFUGATION; DRUG EFFICACY; ENZYME LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTE; FLOW CYTOMETRY; IN VITRO STUDY; LYMPHOCYTE; NATURAL KILLER CELL; PRIORITY JOURNAL;

ADCC; CALCEIN; CHROMIUM RELEASE; MAGNETIC CELL SEPARATION; RAPID HIGH-THROUGHPUT; TETRAMERIC ANTIBODY COMPLEXES;

CENTRIFUGATION, DENSITY GRADIENT; CYTOTOXICITY, IMMUNOLOGIC; HIGH-THROUGHPUT SCREENING ASSAYS; HT29 CELLS; HUMANS; IMMUNOMAGNETIC SEPARATION; K562 CELLS; KILLER CELLS, NATURAL; LYMPHOCYTES;

EID: 84880045270     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.05.001     Document Type: Article

References (25)

1. Beziat V., Duffy D., Quoc S.N., Le Garff-Tavernier M., Decocq J., Combadiere B., Debre P., Vieillard V. CD56brightCD16+ NK cells: a functional intermediate stage of NK cell differentiation. J. Immunol. 2011, 186:6753.
2. Bjorkstrom N.K., Riese P., Heuts F., Andersson S., Fauriat C., Ivarsson M.A., Bjorklund A.T., Flodstrom-Tullberg M., Michaelsson J., Rottenberg M.E., Guzman C.A., Ljunggren H.G., Malmberg K.J. Expression patterns of NKG2A, KIR, and CD57 define a process of CD56dim NK-cell differentiation uncoupled from NK-cell education. Blood 2010, 116:3853.
3. Borella P., Bargellini A., Salvioli S., Medici C.I., Cossarizza A. The use of non-radioactive chromium as an alternative to 51Cr in NK assay. J. Immunol. Methods 1995, 186:101.
4. Busch R., Cesar D., Higuera-Alhino D., Gee T., Hellerstein M.K., McCune J.M. Isolation of peripheral blood CD4(+) T cells using RosetteSep and MACS for studies of DNA turnover by deuterium labeling. J.n Immunol. Methods 2004, 286:97.
5. De Paoli P., Reitano M., Battistin S., Castiglia C., Santini G. Enumeration of human lymphocyte subsets by monoclonal antibodies and flow cytometry: a comparative study using whole blood or mononuclear cells separated by density gradient centrifugation. J. Immunol. Methods 1984, 72:349.
6. English D., Andersen B.R. Single-step separation of red blood cells. Granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque. J. Immunol. Methods 1974, 5:249.
7. Gatti R., Belletti S., Orlandini G., Bussolati O., Dall'Asta V., Gazzola G.C. Comparison of annexin V and calcein-AM as early vital markers of apoptosis in adherent cells by confocal laser microscopy. J. Histochem. Cytochem. 1998, 46:895.
8. Grutzkau A., Radbruch A. Small but mighty: how the MACS-technology based on nanosized superparamagnetic particles has helped to analyze the immune system within the last 20years. Cytometry A 2010, 77:643.
9. Jang Y.Y., Cho D., Kim S.K., Shin D.J., Park M.H., Lee J.J., Shin M.G., Shin J.H., Suh S.P., Ryang D.W. An improved flow cytometry-based natural killer cytotoxicity assay involving calcein AM staining of effector cells. Ann. Clin. Lab. Sci. 2012, 42:42-49.
10. Kono K., Takahashi A., Ichihara F., Sugai H., Fujii H., Matsumoto Y. Impaired antibody-dependent cellular cytotoxicity mediated by herceptin in patients with gastric cancer. Cancer Res. 2002, 62:5813.
11. Levy E.M., Roberti M.P., Mordoh J. Natural killer cells in human cancer: from biological functions to clinical applications. J. Biomed. Biotechnol. 2011, 676198.
12. Lichtenfels R., Biddison W.E., Schulz H., Vogt A.B., Martin R. CARE-LASS (calcein-release-assay), an improved fluorescence-based test system to measure cytotoxic T lymphocyte activity. J. Immunol. Methods 1994, 172:227.
13. Mansour I., Bourin P., Rouger P., Doinel C. A rapid technique for lymphocyte preparation prior to two-color immunofluorescence analysis of lymphocyte subsets using flow cytometry. Comparison with density gradient separation. J. Immunol. Methods 1990, 127:61.
14. Miltenyi S., Muller W., Weichel W., Radbruch A. High gradient magnetic cell separation with MACS. Cytometry 1990, 11:231.
15. Neri S., Mariani E., Meneghetti A., Cattini L., Facchini A. Calcein-acetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants. Clin. Diagn. Lab. Immunol. 2001, 8:1131.
16. Nishimura M., Mitsunaga S., Akaza T., Mitomi Y., Tadokoro K., Juji T. Protection against natural killer cells by interferon-gamma treatment of K562 cells cannot be explained by augmented major histocompatibility complex class I expression. Immunology 1994, 83:75.
17. Palma P.F., Baggio G.L., Spada C., Silva R.D., Ferreira S.I., Treitinger A. Evaluation of annexin V and calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection. Braz. J. Infect. Dis. 2008, 12:108.
18. Roden M.M., Lee K.H., Panelli M.C., Marincola F.M. A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology. J. Immunol. Methods 1999, 226:29.
19. Sanchez-Correa B., Morgado S., Gayoso I., Bergua J.M., Casado J.G., Arcos M.J., Bengochea M.L., Duran E., Solana R., Tarazona R. Human NK cells in acute myeloid leukaemia patients: analysis of NK cell-activating receptors and their ligands. Cancer Immunol. Immunother. 2011, 60:1195.
20. Savary C.A., Phillips J.H., Lotzova E. Inhibition of murine natural killer cell-mediated cytotoxicity by pretreatment with ammonium chloride. J. Immunol. Methods 1979, 25:189.
21. Schlaeth M., Berger S., Derer S., Klausz K., Lohse S., Dechant M., Lazar G.A., Schneider-Merck T., Peipp M., Valerius T. Fc-engineered EGF-R antibodies mediate improved antibody-dependent cellular cytotoxicity (ADCC) against KRAS-mutated tumor cells. Cancer Sci. 2010, 101:1080.
22. Strome S.E., Sausville E.A., Mann D. A mechanistic perspective of monoclonal antibodies in cancer therapy beyond target-related effects. Oncologist 2007, 12:1084.
23. Uellner R., Zvelebil M.J., Hopkins J., Jones J., MacDougall L.K., Morgan B.P., Podack E., Waterfield M.D., Griffiths G.M. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipid-binding C2 domain. EMBO J. 1997, 16:7287.
24. Watanabe M., Kono K., Kawaguchi Y., Mizukami Y., Mimura K., Maruyama T., Izawa S., Fujii H. NK cell dysfunction with down-regulated CD16 and up-regulated CD56 molecules in patients with esophageal squamous cell carcinoma. Dis. Esophagus. 2010, 23:675.
25. Yokoyama W.M., Kim S. Licensing of natural killer cells by self-major histocompatibility complex class I. Immunol. Rev. 2006, 214:143.