An improved flow cytometry-based natural killer cytotoxicity assay involving calcein AM staining of effector cells

Annals of Clinical and Laboratory Science

Volumn 42, Issue 1, 2012, Pages 42-49

  Jang, Youn Young ^a,b   Cho, Duck ^a,b,c   Kim, Sang Ki ^a,d   Shin, Dong Jun ^a   Park, Min Ho ^a,b   Lee, Je Jung ^a,b   Shin, Myung Geun ^b,c   Shin, Jong Hee ^b,c   Suh, Soon Pal ^b,c   Ryang, Dong Wook ^b,c  

^a Chonnam National University Hwasun Hospital and Medical School   (South Korea)

^b Chonnam National University   (South Korea)

^c Chonnam National University Medical School   (South Korea)

^d Kongju National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CALCEIN; PROPIDIUM IODIDE;

ARTICLE; CANCER CELL CULTURE; CELL ACTIVITY; CELL EXPANSION; CELL ISOLATION; CELL STRAIN K 562; CONTROLLED STUDY; CYTOTOXICITY; DENSITY GRADIENT CENTRIFUGATION; EFFECTOR CELL; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; INCUBATION TIME; NATURAL KILLER CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; TARGET CELL;

CELL LINE, TUMOR; CELL SEPARATION; CHROMIUM RADIOISOTOPES; CYTOTOXICITY TESTS, IMMUNOLOGIC; CYTOTOXICITY, IMMUNOLOGIC; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HUMANS; KILLER CELLS, NATURAL; STAINING AND LABELING;

EID: 84863270428     PISSN: 00917370     EISSN: 15508080     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Chang L, Gusewitch GA, Chritton DB, Folz JC, Lebeck LK, Nehlsen-Cannarella SL. Rapid flow cytometric assay for the assessment of natural killer cell activity. J Immunol Methods 1993; 166:45-54.
2. Kane KL, Ashton FA, Schmitz JL, Folds JD. Determination of natural killer cell function by flow cytometry. Clin Diagn Lab Immunol 1996; 3:295-300.
3. von Zons P, Crowley-Nowick P, Friberg D, Bell M, Koldovsky U, Whiteside TL. Comparison of europium and chromium release assays: cytotoxicity in healthy individuals and patients with cervical carcinoma. Clin Diagn Lab Immunol 1997; 4:202-207.
4. Godoy-Ramirez K, Franck K, Gaines H. A novel method for the simultaneous assessment of natural killer cell conjugate formation and cytotoxicity at the single-cell level by multi-parameter flow cytometry. J Immunol Methods 2000; 239:35-44.
5. Vitale M, Zamai L, Neri LM, Manzoli L, Facchini A, Papa S. Natural killer function in flow cytometry: identification of human lymphoid subsets able to bind to the NK sensitive target K562. Cytometry 1991; 12:717-722.
6. Hatam L, Schuval S, Bonagura VR. Flow cytometric analysis of natural killer cell function as a clinical assay. Cytometry 1994; 16:59-68.
7. Cholujova D, Jakubikova J, Kubes M, et al. Comparative study of four fluorescent probes for evaluation of natural killer cell cytotoxicity assays. Immunobiology 2008; 213:629-640.
8. Cho D, Shook DR, Shimasaki N, Chang YH, Fujisaki H, Campana D. Cytotoxicity of activated natural killer cells against pediatric solid tumors. Clin Cancer Res 2010; 16:3901-3909.
9. Hoppner M, Luhm J, Schlenke P, Koritke P, Frohn C. A flowcytometry based cytotoxicity assay using stained effector cells in combination with native target cells. J Immunol Methods 2002; 267:157-163.
10. Kim GG, Donnenberg VS, Donnenberg AD, Gooding W, Whiteside TL. A novel multiparametric flow cytometry-based cytotoxicity assay simultaneously immunophenotypes effector cells: comparisons to a 4 h 51Cr-release assay. J Immunol Methods 2007; 325:51-66.
11. Chung HJ, Park CJ, Lim JH, et al. Establishment of a reference interval for natural killer cell activity through flow cytometry and its clinical application in the diagnosis of hemophagocytic lymphohistiocytosis. Int J Lab Hematol 2010; 32:239-247.
12. Imai C, Iwamoto S, Campana D. Genetic modification of primary natural killer cells overcomes inhibitory signals and induces specific killing of leukemic cells. Blood 2005; 106:376-383.
13. Cho D, Campana D. Expansion and activation of natural killer cells for cancer immunotherapy. Korean J Lab Med 2009; 29:89-96.
14. Roden MM, Lee KH, Panelli MC, Marincola FM. A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology. J Immunol Methods 1999; 226:29-41.
15. Neri S, Mariani E, Meneghetti A, Cattini L, Facchini A. Calcein-acetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants. Clin Diagn Lab Immunol 2001; 8:1131-1135.
16. Dewan MZ, Takada M, Terunuma H, et al. Natural killer activity of peripheral-blood mononuclear cells in breast cancer patients. Biomed Pharmacother 2009; 63:703-706.
17. Callewaert DM, Radcliff G, Waite R, LeFevre J, Poulik MD. Characterization of effector-target conjugates for cloned human natural killer and human lymphokine activated killer cells by flow cytometry. Cytometry 1991; 12:666-676.
18. Radcliff G, Waite R, LeFevre J, Poulik MD, Callewaert DM. Quantification of effector/target conjugation involving natural killer (NK) or lymphokine activated killer (LAK) cells by twocolor flow cytometry. J Immunol Methods 1991; 139:281-292.
19. Motzer SA, Tsuji J, Hertig V, Johnston SK, Scanlan J. Natural killer cell cytotoxicity: a methods analysis of 51chromium release versus flow cytometry. Biol Res Nurs 2003; 5:142-152.