Egc characterization of enterotoxigenic Staphylococcus aureus isolates obtained from raw milk and cheese

International Journal of Food Microbiology

Volumn 165, Issue 3, 2013, Pages 227-230

  Viçosa, Gabriela Nogueira ^a   Le Loir, Alban ^a,b,c   Le Loir, Yves ^b,c   de Carvalho, Antônio Fernandes ^a   Nero, Luís Augusto ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

^b UMR1253   (France)

^c AGROCAMPUS OUEST   (France)

Author keywords

Cheese; Egc; Enterotoxin gene cluster; Milk; PCR RFLP; Staphylococcus aureus

Indexed keywords

STAPHYLOCOCCUS ENTEROTOXIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CHEESE; CONTROLLED STUDY; ENTEROTOXIN GENE CLUSTER; GENE CLUSTER; GENE FREQUENCY; GENE LOCUS; GENE SEQUENCE; GENETIC VARIABILITY; GENOTYPE; MILK; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS AUREUS;

EID: 84879574662     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2013.05.023     Document Type: Article

References (28)

1. Aydin A., Sudagidan M., Muratoglu K. Prevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Region of Turkey. International Journal of Food Microbiology 2011, 148:99-106.
2. Bania J., Dabrowska A., Bystron J., Korzekwa K., Chrzanowska J., Molenda J. Distribution of newly described enterotoxin-like genes in Staphylococcus aureus from food. International Journal of Food Microbiology 2006, 108:36-41.
3. Blaiotta G., Ercolini D., Pennacchia C., Fusco V., Casaburi A., Pepe O., Villani F. PCR detection of staphylococcal enterotoxin genes in Staphylococcus spp. strains isolated from meat and dairy products. Evidence for new variants of seG and seI in S. aureus AB-8802. Journal of Applied Microbiology 2004, 97:719-730.
4. Bystron J., Podkowik M., Piasecki T., Wieliczko A., Molenda J., Bania J. Genotypes and enterotoxin gene content of S. aureus isolates from poultry. Veterinary Microbiology 2010, 144:498-501.
5. Carmo L.S., Dias R.S., Linardi V.R., Sena M.J., Santos D.A., Faria M.E., Pena E.C., Jett M., Heneine L.G. Food poisoning due to enterotoxigenic strains of Staphylococcus present in Minas cheese and raw milk in Brazil. Food Microbiology 2002, 19:9-14.
6. (v) gene using PCR-RFLP. Journal of Medical Microbiology 2007, 56:208-216.
7. Collery M.M., Smyth D.S., Tumilty J.J.G., Twohig J.M., Smyth C.J. Associations between enterotoxin gene cluster types egc1, egc2 and egc3, agr types, enterotoxin and enterotoxin-like gene profiles, and molecular typing characteristics of human nasal carriage and animal isolates of Staphylococcus aureus. Journal of Medical Microbiology 2009, 58:13-25.
8. Cretenet M., Even S., Le Loir Y. Unveiling Staphylococcus aureus enterotoxin production in dairy products: a review of recent advances to face new challenges. Dairy Science & Technology 2011, 91:127-150.
9. Elazhari M., Elhabchi D., Zerouali K., Dersi N., Elmalki A., Hassar M., Saile R., Timinouni M. Prevalence and distribution of superantigen toxin genes in clinical community isolates of Staphylococcus aureus. Journal of Bacteriology & Parasitology 2011, 2:1000107.
10. Gharsa H., Ben Slama K., Lozano C., Gomez-Sanz E., Klibi N., Ben Sallem R., Gomez P., Zarazaga M., Boudabous A., Torres C. Prevalence, antibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia. Veterinary Microbiology 2012, 156:367-373.
11. Ikeda T., Tamate N., Yamaguchi K., Makin S. Mass outbreak of food poisoning disease caused by small amounts of staphylococcal enterotoxins A and H. Applied and Environmental Microbiology 2005, 71:2793-2795.
12. Jarraud S., Peyrat M.A., Lim A., Tristan A., Bes M., Mougel C., Etienne J., Vandenesch F., Bonneville M., Lina G. egc, A highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus. Journal of Immunology 2001, 166:669-677.
13. Jarraud S., Mougel C., Thioulouse J., Lina G., Meugnier H., Forey F., Nesme X., Etienne J., Vandenesch F. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (Alleles), and human disease. Infection and Immunity 2002, 70:631-641.
14. Larkin E.A., Carman R.J., Krakauer T., Stiles B.G. Staphylococcus aureus: the toxic presence of a pathogen extraordinaire. Current Medicinal Chemistry 2009, 16:4003-4019.
15. Lawrynowicz-Paciorek M., Kochman M., Piekarska K., Grochowska A., Windyga B. The distribution of enterotoxin and enterotoxin-like genes in Staphylococcus aureus strains isolated from nasal carriers and food samples. International Journal of Food Microbiology 2007, 117:319-323.
16. Le Loir Y., Baron F., Gautier M. Staphylococcus aureus and food poisoning. Genetics and Molecular Research 2003, 2:63-76.
17. Letertre C., Perelle S., Dilasser F., Fach P. Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus. Journal of Applied Microbiology 2003, 95:38-43.
18. McLauchlin J., Narayanan G.L., Mithani V., O'Neill G. The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction. Journal of Food Protection 2000, 63:479-488.
19. Mehrotra M., Wang G., Johnson W.M. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. Journal of Clinical Microbiology 2000, 38:1032-1035.
20. Mempel M., Lina G., Hojka M., Schnopp C., Seidl H.P., Schafer T., Ring J., Vandenesch F., Abeck D. High prevalence of superantigens associated with the egc locus in Staphylococcus aureus isolates from patients with atopic eczema. European Journal of Clinical Microbiology & Infectious Diseases 2003, 22:306-309.
21. Nashev D., Toshkova K., Bizeva L., Akineden O., Laemmler C., Zschoeck M. Distribution of enterotoxin genes among carriage- and infection-associated isolates of Staphylococcus aureus. Letters in Applied Microbiology 2007, 45:681-685.
22. Schelin J., Wallin-Carlquist N., Cohn M.T., Lindqvist R., Barker G.C., Radstrom P. The formation of Staphylococcus aureus enterotoxin in food environments and advances in risk assessment. Virulence 2011, 2:580-592.
23. Smyth D.S., Hartigan P.J., Meaney W.J., Ftzgerald J.R., Deobald C.F., Bohach G.A., Smyth C.J. Superantigen genes encoded by the egc cluster and SaPlbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry. Journal of Medical Microbiology 2005, 54:401-411.
24. Thomas D.Y., Jarraud S., Lemercier B., Cozon G., Echasserieau K., Etienne J., Gougeon M.-L., Lina G., Vandenesch F. Staphylococcal enterotoxin-like toxins U2 and V, two new staphylococcal superantigens arising from recombination within the enterotoxin gene cluster. Infection and Immunity 2006, 74:4724-4734.
25. Vautor E., Magnone V., Rios G., Le Brigand K., Bergonier D., Lina G., Meugnier H., Barbry P., Thiery R., Pepin M. Genetic differences among Staphylococcus aureus isolates from dairy ruminant species: a single-dye DNA microarray approach. Veterinary Microbiology 2009, 133:105-114.
26. Viçosa G.N., Moraes P.M., Yamazi A.K., Nero L.A. Enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese: an evaluation of Baird-Parker agar, Rabbit Plasma Fibrinogen agar and the Petrifilm™ Staph Express count system. Food Microbiology 2010, 27:447-452.
27. Zhang C., Shen Y., Dong M. Distribution, polymorphism and temporal expression of egc in Staphylococcus aureus isolates from various foods in China. Food Control 2013, 29:279-285.
28. Zocche F., Bastos C.P., Silva W.P. Detecção de genes do cluster egc em Staphylococcus aureus isolados de alimentos de origem animal. Ciência Rural 2010, 40:1134-1140.