A Genome-Wide siRNA Screen to Identify Host Factors Necessary for Growth of the Parasite Toxoplasma gondii

PLoS ONE

Volumn 8, Issue 6, 2013, Pages

  Moser, Lindsey A ^a   Pollard, Angela M ^b   Knoll, Laura J ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b AGILE SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HOST FACTOR; SMALL INTERFERING RNA;

ARTICLE; CELL DEATH; GENE FUNCTION; GENETIC ASSOCIATION; GENETIC IDENTIFICATION; GROWTH; HOST CELL; HUMAN; HUMAN CELL; MOLECULAR LIBRARY; NONHUMAN; PARASITE DEVELOPMENT; PROTEIN DEGRADATION; RNA ANALYSIS; RNA TRANSCRIPTION; RNA TRANSLATION; TOXOPLASMA GONDII;

ANIMALS; CELL DEATH; CELL LINE, TUMOR; CELLS, CULTURED; FIBROBLASTS; GENOME-WIDE ASSOCIATION STUDY; HELA CELLS; HOST-PARASITE INTERACTIONS; HUMANS; PARASITES; PROTEINS; RNA, SMALL INTERFERING; TOXOPLASMA;

ANIMALIA; TOXOPLASMA GONDII;

EID: 84879517056     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0068129     Document Type: Article

References (35)

1. Dubey JP, (2009) History of the discovery of the life cycle of Toxoplasma gondii. Int J Parasitol 39: 877-882.
2. Blader IJ, Manger ID, Boothroyd JC, (2001) Microarray analysis reveals previously unknown changes in Toxoplasma gondii-infected human cells. J Biol Chem 276: 24223-24231.
3. Gail M, Gross U, Bohne W, (2001) Transcriptional profile of Toxoplasma gondii-infected human fibroblasts as revealed by gene-array hybridization. Mol Genet Genomics 265: 905-912.
4. Nash PB, Purner MB, Leon RP, Clarke P, Duke RC, et al. (1998) Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis. J Immunol 160: 824-1830.
5. Shapira S, Harb OS, Margarit J, Matrajt M, Han J, et al. (2005) Initiation and termination of NF-kappaB signaling by the intracellular protozoan parasite Toxoplasma gondii. J Cell Sci 118: 3501-3508.
6. Kim SK, Fouts AE, Boothroyd JC, (2007) Toxoplasma gondii dysregulates IFN-gamma-inducible gene expression in human fibroblasts: insights from a genome-wide transcriptional profiling. J Immunol 178: 5154-5165.
7. Nelson MM, Jones AR, Carmen JC, Sinai AP, Burchmore R, et al. (2008) Modulation of the host cell proteome by the intracellular apicomplexan Toxoplasma gondii. Infect Immun 76: 828-44.
8. Xiao J, Jones-Brando L, Talbot CC, Yolken RH, (2011) Differential effects of three canonical Toxoplasma strains on gene expression in human neuroepithelial cells. Infect Immun 79: 1363-1373.
9. Karlas A, Machuy N, Shin Y, Pleisner KP, Artarini A, et al. (2010) Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication. Nature 463: 818-822.
10. Jayaswal S, Kamal MA, Dua R, Gupta S, Majumdar T, et al. (2010) Identification of host-dependent survival factors for intracellular Mycobacterium tuberculosis through an siRNA screen. PLoS Path 6: e1000839.
11. Kumar M, Khan FG, Sharma S, Kumar R, Faujdar J, et al. (2010) Identification of Mycobacterium tuberculosis genes preferentially expressed during human infection. Microb Pathog 50: 31-38.
12. Genovesio A, Giardini MA, Kwon YJ, de Macedo Dossin F, Choi SY, et al. (2011) Visual genome-wide RNAi screening to identify human host factors required for Trypanosoma cruzi infection. PLoS One 6: e19733.
13. Prudêncio M, Rodrigues CD, Hannus M, Martin C, Real E, et al. (2008) Kinome-wide RNAi screen implicates at least 5 host hepatocyte kinases in Plasmodium sporozoite infection. PLoS Pathog 4: e1000201.
14. Donald RG, Carter D, Ullman B, Roos DS, (1996) Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphribosyltransferase gene. Use as a selectable marker for stable transformation. J Bio Chem 271: 14010-14019.
15. Chtanova T, Schaeffer M, Han SJ, van Dooren GG, Nollmann M, et al. (2008) Dynamics of neutrophil migration in lymph nodes during infection. Immunity 29: 487-496.
16. Molestina RE, El-Guendy N, Sinai AP, (2008) Infection with Toxoplasma gondii results in dysregulation of the host cell cycle. Cell Microbiol. 10: 1153-65.
17. Lavine MD, Arrizabalaga G, (2009) Induction of mitotic S-phase of host and neighboring cells by Toxoplasma gondii enhances parasite invasion. Mol Biochem Parasitol 164: 95-9.
18. Walker ME, Hjort EE, Smith SS, Tripathi A, Hornick JE, et al. (2008) Toxoplasma gondii actively remodels the microtubule network in host cells. Microbes Infect 10: 1440-9.
19. Sato M, Toda T, (2010) Space shuttling in the cell: nucleocytoplasmic transport and microtubule organization during the cell cycle. Nucleus 1: 231-6.
20. Hautbergue GM, Hung ML, Golovanov AP, Lian LY, Wilson SA, (2008) Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP. Proc Natl Acad Sci USA 105(13): 5154-9.
21. Zolotukhin AS, Uranishi H, Lindtner S, Bear J, Pavlakis GN, et al. (2009) Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA. Nuc Acid Res 37: 7151-7162.
22. Pendergast AM, (1996) Nuclear tyrosine kinases: from Abl to WEE1. Curr Opin Cell Biol 8: 174-181.
23. Sørensen CS, Syljuåsen RG, (2012) Safeguarding genome integrity: the checkpoint kinases ATR, CHK1 and WEE1 restrain CDK activity during normal DNA replication. Nuc Acid Res 40: 477-86.
24. Parsons BD, Schindler A, Evans DH, Foley E, (2009) A direct phenotypic comparison of siRNA pools and multiple individual duplexes in a functional assay. PLoS ONE 4: e8471.
25. Shibuya T, Tange TØ, Sonenberg N, Moore MJ, (2004) eIF4AIII binds spliced mRNA in the exon junction complex and is essential for nonsense-mediated decay. Nat Structl Mol Biol 11: 346-351.
26. Bachi A, Braun IC, Rodrigues JP, Panté N, Ribbeck K, et al. (2000) The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates. RNA 6: 136-158.
27. Custódio N, Carvalho C, Condado I, Antoniou M, Blencowe BJ, et al. (2004) In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei. RNA 10: 622-633.
28. Tseng SS, Weaver PL, Liu Y, Hitomi M, Tartakoff AM, et al. (1998) Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export. EMBO J 17: 2651-2662.
29. Montpetit B, Thomsen ND, Helmke KJ, Seeliger MA, Berger JM, et al. (2011) A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export. Nature 472: 238-242.
30. Sibley LD, Andrews NW, (2000) Cell invasion by un-palatable parasites. Traffic 1: 100-106.
31. Saeij JP, Boyle JP, Coller S, Taylor S, Sibley LD, et al. (2006) Polymorphic secreted kinases are key virulence factors in toxoplasmosis. Science 314: 1780-1783.
32. Ahn HJ, Kim S, Nam HW, (2007) Nucleolar translocalizaton of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells. Korean J Parasitol 45: 165-174.
33. Gilbert LA, Ravindran S, Turetzky JM, Boothroyd JC, Bradly PJ, (2007) Toxoplasma gondii targets a protein phophatase 2C to the nuclei of infected host cells. Euk Cell 6: 73-83.
34. Saeij JP, Boyle JP, Boothroyd JC, (2005) Differences among the three major strains of Toxoplasma gondii and their specific interactions with the infected host. Trends Parasitol. 21: 476-81.
35. Hill RD, Gouffon JS, Saxton AM, Su C, (2012) Differential gene expression in mice infected with distinct Toxoplasma strains. Infect Immun. 80: 968-74.