A validation study of the Nucleix DSI-Semen kit - A methylation-based assay for semen identification

International Journal of Legal Medicine

Volumn 127, Issue 2, 2013, Pages 299-308

  Larue, Bobby L ^a   King, Jonathan L ^a   Budowle, Bruce ^a  

^a UNIVERSITY OF NORTH TEXAS   (United States)

Author keywords

CpG methylation; Degraded DNA; Forensic DNA analysis; Forensic tissue typing; Methylation patterns; Semen; Sexual assault

Indexed keywords

DNA; MICROSATELLITE DNA; PRIMER DNA; RESTRICTION ENDONUCLEASE;

ANIMAL; ARTICLE; BLOOD ANALYSIS; COMPUTER PROGRAM; DNA DEGRADATION; DNA FINGERPRINTING; DNA FRAGMENTATION; DNA METHYLATION; DRUG EFFECT; ELECTROPHORESIS; EQUIPMENT; FORENSIC GENETICS; HUMAN; LIMIT OF DETECTION; MALE; METABOLISM; MOUTH MUCOSA; MULTIPLEX POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SALIVA; SPERM; STATISTICAL MODEL; ULTRAVIOLET RADIATION; VALIDATION STUDY;

ANIMALS; BLOOD CHEMICAL ANALYSIS; DNA; DNA DEGRADATION, NECROTIC; DNA FINGERPRINTING; DNA FRAGMENTATION; DNA METHYLATION; DNA PRIMERS; DNA RESTRICTION ENZYMES; ELECTROPHORESIS; FORENSIC GENETICS; HUMANS; LIKELIHOOD FUNCTIONS; LIMIT OF DETECTION; MALE; MICROSATELLITE REPEATS; MOUTH MUCOSA; MULTIPLEX POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SALIVA; SEMEN; SOFTWARE; ULTRAVIOLET RAYS;

EID: 84879505808     PISSN: 09379827     EISSN: 14371596     Source Type: Journal    
DOI: 10.1007/s00414-012-0760-0     Document Type: Article

References (24)

1. Truman JL (2011) Criminal victimization (2009) DIANE
2. Hochmeister MN, Budowle B, Rudin O, Gehrig C, Borer U, Thali M, Dirnhofer R (1999) Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid. J Forensic Sci 44:1057-1060
3. Sato I, Kojima K, Yamasaki T, Yoshida K, Yoshiike M, Takano S, Mukai T, Iwamoto T (2004) Rapid detection of semenogelin by one-step immunochromatographic assay for semen identification. J Immunol Methods 287(1-2):137-145
4. Virkler K, Lednev IK (2008) Raman spectroscopy offers great potential for the nondestructive confirmatory identification of body fluids. Forensic Sci Int 181(1):e1-e5
5. Virkler K, Lednev IK (2009) Analysis of body fluids for forensic purposes: from laboratory testing to non-destructive rapid confirmatory identification at a crime scene. Forensic Sci Int 188(1):1-17
6. Sanders CT, Sanchez N, Ballantyne J, Peterson DA (2006) Laser microdissection separation of pure spermatozoa from epithelial cells for short tandem repeat analysis. J Forensic Sci 51(4):748-757
7. Jones DA (1972) Blood samples: probability of discrimination. J Forensic Sci Soc 12(2):355-359
8. National Research Council WDC. Committee on DNA Technology in Forensic Science (1992) DNA technology in forensic science. The National Academies, Washington, DC
9. Juusola J, Ballantyne J (2003) Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification. Forensic Sci Int 135(2):85-96
10. Juusola J, Ballantyne J (2005) Multiplex mRNA profiling for the identification of body fluids. Forensic Sci Int 152(1):1-12
11. Setzer M, Juusola J, Ballantyne J (2008) Recovery and stability of RNA in vaginal swabs and blood, semen, and saliva stains. J Forensic Sci 53(2):296-305
12. Dixon LA, Murray CM, Archer EJ, Dobbins AE, Koumi P, Gill P (2005) Validation of a 21-locus autosomal SNP multiplex for forensic identification purposes. Forensic Sci Int 154(1):62-77
13. Moretti T, Baumstark A, Defenbaugh D, Keys K, Smerick J, Budowle B (2001) Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. J Forensic Sci 46(3):647
14. LaFountain MJ, Schwartz MB, Svete PA, Walkinshaw MA, Buel E (2001) TWGDAM validation of the AmpFeSTR Profiler Plus and AmpFeSTR COfiler STR multiplex systems using capillary electrophoresis. J Forensic Sci 46(5):1191-1198
15. LaRue BL, Ge J, King JL, Budowle B (2012) A validation study of the Qiagen Investigator DIPplex® kit; an INDEL-based assay for human identification. Int J Legal Med 1-8
16. Pereira R, Phillips C, Alves C, Amorim A, Carracedo Á, Gusmão L (2009) Insertion/deletion polymorphisms: a multiplex assay and forensic applications. Forensic Sci Int Genet Suppl Ser 2(1):513-515
17. Frumkin D, Wasserstrom A, Budowle B, Davidson A (2011) DNA methylation-based forensic tissue identification. Forensic Sci Int Genet 5:517-524
18. Frumkin D, Wasserstrom A, Davidson A, Grafit A (2010) Authentication of forensic DNA samples. Forensic Sci Int Genet 4(2):95-103
19. Lee HY, Park MJ, Choi A, An JH, Yang WI, Shin KJ (2012) Potential forensic application of DNA methylation profiling to body fluid identification. Int J Legal Med 126:55-62
20. Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB (1996) Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci 93(18):9821
21. Akane A, Matsubara K, Nakamura H, Takahashi S, Kimura K (1994) Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J Forensic Sci 39:362-372
22. Alaeddini R (2012) Forensic implications of PCR inhibition - a review. Forensic Sci Int Genet 6(3):297-305
23. Opel KL, Chung D, McCord BR (2010) A study of PCR inhibition mechanisms using real time PCR. J Forensic Sci 55(1):25-33
24. Tebbe CC, Vahjen W (1993) Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast. Appl Environ Microbiol 59(8):2657-2665