Validation of short tandem repeats (STRs) for forensic usage: Performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples

Journal of Forensic Sciences

Volumn 46, Issue 3, 2001, Pages 647-660

  Moretti, T R ^a   Baumstark, A L ^a   Defenbaugh, D A ^a   Keys, K M ^a   Smerick, J B ^b   Budowle, B ^b  

^a FBI LABORATORY   (United States)

^b Laboratory Division   (United States)

Author keywords

Core CODIS loci; CSF1PO; D13S317; D16S539; D18S51; D21S11; D3S1358; D5S818; D7S820; D8S1179; DNA typing; FGA; Fluorescence; Forensic science; Multiplex amplification; Polymerase chain reaction; Short tandem repeats; TH01; TPOX; Validation; VWA

Indexed keywords

ARTICLE; BINDING SITE; CONTROLLED STUDY; DEVICE; DNA BINDING; DNA DETERMINATION; DNA SEQUENCE; FLUORESCENCE; FORENSIC MEDICINE; GENE AMPLIFICATION; GENE DUPLICATION; GENE LOCUS; GENE TRANSLOCATION; GENETIC ANALYSIS; GENOTYPE; HETEROZYGOSITY; HUMAN; PERFORMANCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SIMULATION; TANDEM REPEAT; VALIDATION PROCESS;

EID: 0034990151     PISSN: 00221198     EISSN: None     Source Type: Journal    
DOI: 10.1520/jfs15018j     Document Type: Article

References (41)

1. Population data on the thirteen CODIS core short tandem repeat loci in African Americans, U.S. Caucasians, Hispanics, Bahamians, Jamaicans and Trinidadians
2. Validation studies of the CTT STR multiplex system
3. Validation of highly polymorphic fluorescent multiplex short tandem repeat systems using two generations of DNA sequencers
4. Validation of a highly discriminating multiplex short tandem repeat amplification system for human identification
5. The validation of short tandem repeat (STR) loci for use in forensic casework
6. Validation of multiplex polymorphism STR amplification sets developed for personal identification applications
7. The validation of a 7-locus multiplex STR test for use in forensic case- work. (II) Artefacts, casework studies and success rates
8. The validation of a 7-locus multiplex STR test for use in forensic casework. (I) Mixtures, ageing, degradation and species specificity
9. TWGDAM validation of the AmpFℓSTR Blue PCR amplification kit for forensic casework analysis
10. DNA extraction strategies for amplified fragment length polymorphism analysis
11. Use of Chelex to improve the PCR signal from a small number of cells
12. Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material
13. Simple protocols for typing forensic biological evidence: Chemiluminescent detection for human DNA quantitation and RFLP analyses and manual typing of PCR amplified polymorphisms
14. A simple method for quantifying human genomic DNA in forensic specimen extracts
15. D1S80 typing of DNA from simulated forensic specimens
16. Validation and population studies of the loci LDLR, GYPA, HBGG, D7S8, and Gc (PM loci), and HLA-DQα using a multiplex amplification and typing procedure
17. Forensic application of DNA "finger-prints."
18. Enhancement of PCR amplification yield and specificity using AmpliTaq Gold™ DNA polymerase
19. Typing of DNA extracted from compact bone tissue from human remains
20. Validation of STR typing by capillary electrophoresis
21. Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA
22. Preferential PCR amplification of alleles: Mechanisms and solutions
23. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases
24. Substrate nucleotide-determined non-templated addition of adenine by Taq DNA polymerase: Implications for PCR-based genotyping and cloning
25. Somatic mutation processes at a human minisatellite
26. Deoxyribonucleic acid (DNA) analysis by restriction fragment length polymorphisms of blood and other body fluid stains subjected to contamination and environmental insults
27. Multiplex amplification and typing procedure for the loci D1S80 and amelogenin
28. Approaches to genetic analyses: Forensics
29. Validation studies on the analysis of the HLA DQα locus using the polymerase chain reaction
30. Evaluation of deoxyribonucleic acid (DNA) isolated from human blood-stains exposed to ultraviolet light, light, heat, humidity, and soil contamination
31. The effects of environment and substrata on deoxyribonucleic acid (DNA): The use of casework samples from New York City
32. HUMTH01 validation studies: Effect of substrate, environment and mixtures
33. Investigation of species specificity using nine PCR-based-human STR systems