Single-molecule motility: Statistical analysis and the effects of track length on quantification of processive motion

Biophysical Journal

Volumn 104, Issue 12, 2013, Pages 2651-2661

  Thompson, Andrew R ^a   Hoeprich, Gregory J ^a   Berger, Christopher L ^a  

^a UNIVERSITY OF VERMONT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KINESIN;

ANIMAL; BIOLOGICAL MODEL; CHEMISTRY; HUMAN; KINETICS; MICROTUBULE; MOTION; STATISTICAL MODEL;

ANIMALS; HUMANS; KINESIN; KINETICS; MICROTUBULES; MODELS, BIOLOGICAL; MODELS, STATISTICAL; MOTION;

EID: 84879200496     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2013.05.022     Document Type: Article

References (36)

1. D.M. Warshaw, and G.G. Kennedy K.M. Trybus Differential labeling of myosin V heads with quantum dots allows direct visualization of hand-over-hand processivity Biophys. J. 88 2005 L30 L32
2. S. Shastry, and W.O. Hancock Neck linker length determines the degree of processivity in kinesin-1 and kinesin-2 motors Curr. Biol. 20 2010 939 943
3. S. Shastry, and W.O. Hancock Interhead tension determines processivity across diverse N-terminal kinesins Proc. Natl. Acad. Sci. USA 108 2011 16253 16258
4. R. Dixit, and J.L. Ross E.L. Holzbaur Differential regulation of dynein and kinesin motor proteins by Tau Science 319 2008 1086 1089
5. D.P. McVicker, L.R. Chrin, and C.L. Berger The nucleotide-binding state of microtubules modulates kinesin processivity and the ability of Tau to inhibit kinesin-mediated transport J. Biol. Chem. 286 2011 42873 42880
6. M. Vershinin, and B.C. Carter S.P. Gross Multiple-motor based transport and its regulation by Tau Proc. Natl. Acad. Sci. USA 104 2007 87 92
7. R.H. Miller, R.J. Lasek, and M.J. Katz Preferred microtubules for vesicle transport in lobster axons Science 235 1987 220 222
8. T. Nakata, and S. Niwa N. Hirokawa Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport J. Cell Biol. 194 2011 245 255
9. D. Cai, and D.P. McEwen K.J. Verhey Single molecule imaging reveals differences in microtubule track selection between kinesin motors PLoS Biol. 7 2009 e1000216
10. N.A. Reed, and D. Cai K.J. Verhey Microtubule acetylation promotes kinesin-1 binding and transport Curr. Biol. 16 2006 2166 2172
11. Y. Konishi, and M. Setou Tubulin tyrosination navigates the kinesin-1 motor domain to axons Nat. Neurosci. 12 2009 559 567
12. J.W. Hammond, and C.F. Huang K.J. Verhey Posttranslational modifications of tubulin and the polarized transport of kinesin-1 in neurons Mol. Biol. Cell 21 2010 572 583
13. G. Morfini, and G. Pigino S.T. Brady Tau binding to microtubules does not directly affect microtubule-based vesicle motility J. Neurosci. Res. 85 2007 2620 2630
14. S. Courty, and C. Luccardini M. Dahan Tracking individual kinesin motors in living cells using single quantum-dot imaging Nano Lett. 6 2006 1491 1495
15. S.R. Nelson, and M.Y. Ali D.M. Warshaw Random walk of processive, quantum dot-labeled myosin Va molecules within the actin cortex of COS-7 cells Biophys. J. 97 2009 509 518
16. J. Yoo, and T. Kambara H. Higuchi Intracellular imaging of targeted proteins labeled with quantum dots Exp. Cell Res. 314 2008 3563 3569
17. D. Cai, K.J. Verhey, and E. Meyhöfer Tracking single kinesin molecules in the cytoplasm of mammalian cells Biophys. J. 92 2007 4137 4144
18. S.T. Brady, R.J. Lasek, and R.D. Allen Video microscopy of fast axonal transport in extruded axoplasm: a new model for study of molecular mechanisms Cell Motil. 5 1985 81 101
19. T.J. Gould, V.V. Verkhusha, and S.T. Hess Imaging biological structures with fluorescence photoactivation localization microscopy Nat. Protoc. 4 2009 291 308
20. W. Yu, and P.W. Baas Changes in microtubule number and length during axon differentiation J. Neurosci. 14 1994 2818 2829
21. E. Meijering, O. Dzyubachyk, and I. Smal Methods for cell and particle tracking Methods Enzymol. 504 2012 183 200
22. R.D. Vale, and R.A. Milligan The way things move: looking under the hood of molecular motor proteins Science 288 2000 88 95
23. A. Yildiz, and M. Tomishige P.R. Selvin Kinesin walks hand-over-hand Science 303 2004 676 678
24. S.M. Block, L.S. Goldstein, and B.J. Schnapp Bead movement by single kinesin molecules studied with optical tweezers Nature 348 1990 348 352
25. R.D. Vale, and T. Funatsu T. Yanagida Direct observation of single kinesin molecules moving along microtubules Nature 380 1996 451 453
26. G.P. McCabe, and D.S. Moore Introduction to the Practice of Statistics 2006 W. H. Freeman New York
27. S. Nagy, and B.L. Ricca R.S. Rock A myosin motor that selects bundled actin for motility Proc. Natl. Acad. Sci. USA 105 2008 9616 9620
28. W.J. Walter, and V. Beránek S. Diez Tubulin acetylation alone does not affect kinesin-1 velocity and run length in vitro PLoS ONE 7 2012 e42218
29. P.I. Good Permutation Tests: a Practical Guide to Resampling Methods for Testing Hypotheses 2000 Springer New York
30. M. Gyimesi, and K. Sarlós M. Kovács Streamlined determination of processive run length and mechanochemical coupling of nucleic acid motor activities Nucleic Acids Res. 38 2010 e102
31. Y. Jeune-Smith, and H. Hess Engineering the length distribution of microtubules polymerized in vitro Soft Matter 8 2010 1778 1784
32. M. Dogterom, and S. Leibler Physical aspects of the growth and regulation of microtubule structures Phys. Rev. Lett. 70 1993 1347 1350
33. D.J. Sharp, and J.L. Ross Microtubule-severing enzymes at the cutting edge J. Cell Sci. 125 2012 2561 2569
34. J.L. Ross, M.Y. Ali, and D.M. Warshaw Cargo transport: molecular motors navigate a complex cytoskeleton Curr. Opin. Cell Biol. 20 2008 41 47
35. S. Dunn, and E.E. Morrison M. Peckham Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells J. Cell Sci. 121 2008 1085 1095
36. J.L. Ross, and H. Shuman Y.E. Goldman Kinesin and dynein-dynactin at intersecting microtubules: motor density affects dynein function Biophys. J. 94 2008 3115 3125