Effect of disruption of Pichia pastoris YPS1 gene on viability and production of recombinant proteins

Russian Journal of Genetics

Volumn 49, Issue 6, 2013, Pages 602-608

  Sazonova, E A ^a   Zobnina, A E ^a   Padkina, M V ^a  

^a ST PETERSBURG STATE UNIVERSITY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; ALBURON PROTEIN; ALPHA INTERFERON; CALCIUM CHLORIDE; CHIMERIC PROTEIN; DODECYL SULFATE SODIUM; FUNGAL PROTEIN; INTERFERON ALPHA 16; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; YAPSIN 1;

ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; FUNGAL CELL WALL; FUNGAL STRAIN; FUNGAL VIABILITY; FUNGUS GROWTH; GENE; GENE DISRUPTION; GENETIC TRAIT; IFNA16 GENE; NONHUMAN; PHENOTYPE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; YPS1 GENE;

PICHIA PASTORIS;

EID: 84879140768     PISSN: 10227954     EISSN: 16083369     Source Type: Journal    
DOI: 10.1134/S1022795413040121     Document Type: Article

References (28)

1. Cereghino, G. P., Cereghino, J. L., Ilgen, C., and Cregg, J. M., Production of Recombinant Proteins in Fermenter Cultures of the Yeast Pichia pastoris, Curr. Opin. Biotechnol., 2002, vol. 13, pp. 329-332.
2. Werten, M. W., Bosch, T. J., Wind, R. D., et al., High-Yield Secretion of Recombinant Gelatins by Pichia pastoris, Yeast, 1999, vol. 15, pp. 1087-1096.
3. Shapiro, R. I., Wen, D., Levesque, M., et al., Expression of Sonic Hedgehog-Fc Fusion Protein in Pichia pastoris: Identification and Control of Post-Translational, Chemical, and Proteolytic Modifications, Protein Expres. Purif., 2003, vol. 29, pp. 272-283.
4. Boehm, T., Pirie-Shepherd, S., Trinh, L. B., et al., Disruption of the KEX1 Gene in Pichia pastoris Allows Expression of Full-Length Murine and Human Endostatin, Yeast, 1999, vol. 15, pp. 563-572.
5. Issaly, N., Solsona, O., Joudrier, P., et al., Optimization of the Wheat Puroindoline-a Production in Pichia pastoris, J. Appl. Microbiol., 2001, vol. 90, pp. 397-406.
6. Jahic, M., Gustavsson, M., Jansen, A. K., et al., Analysis and Control of Proteolysis of a Fusion Protein in Pichia pastoris Fed-Batch Processes, J. Biotechnol., 2003, vol. 102, pp. 45-53.
7. Li, Z., Xiong, F., Lin, Q., et al., Low-Temperature Increases the Yield of Biologically Active Herring Antifreeze Protein in Pichia pastoris, Protein Expres. Purif., 2001, vol. 21, pp. 438-445.
8. Kobayashi, K., Kuwae, S., Ohya, T., et al., High-Level Expression of Recombinant Human Serum Albumin from the Methylotrophic Yeast Pichia pastoris with Minimal Protease Production and Activation, J. Biosci. Bioeng., 2000, vol. 89, pp. 55-61.
9. Kang, H. A., Kim, S. J., Choi, E. S., et al., Efficient Production of Intact Human Parathyroid Hormone in a Saccharomyces cerevisiae Mutant Deficient in Yeast Aspartic Protease 3 (YAP3), Appl. Microbiol. Biotechnol., 1998, vol. 50, pp. 187-192.
10. Kerry-Williams, S. M., Gilbert, S. C., Evans, L. R., et al., Disruption of the Saccharomyces cerevisiae YAP3 Gene Reduces the Proteolytic Degradation of Secreted Recombinant Human Albumin, Yeast, 1998, vol. 14, pp. 161-169.
11. Werten, M. and Wolf, F. A., Reduced Proteolysis of Secreted Gelatin and Yps1-Mediated Alpha-Factor Leader Processing in a Pichia pastoris kex2 Disruptant, Appl. Environ. Microbiol., 2005, vol. 71, pp. 2310-2317.
12. Padkina, M. V., Karabel'skii, A. V., Zinov'eva, Yu. G., et al., RF Patent 2373286, 2007.
13. Karabel'skii, A. V. and Padkina, M. V., Purification of the Chimeric Protein Alburon16 from a Culture Medium of the Yeast Pichia pastoris, Prikl. Biokhim. Mikrobiol., 2012, vol. 48, no. 4, pp. 457-462.
14. Yao, X. Q., Zhao, H. L., Xue, C., et al., Degradation of HSA-AX15(R13K) When Expressed in Pichia pastoris Can Be Reduced via the Disruption of YPS1 Gene in This Yeast, J. Biotechnol., 2009, vol. 139, pp. 131-136.
15. Gagnon-Arsenault, I., Tremblay, J., and Bourbonnais, Y., Fungal Yapsins and Cell Wall: A Unique Family of Aspartic Peptidases for a Distinctive Cellular Function, FEMS Yeast Res., 2006, vol. 6, pp. 966-978.
16. Miller, K. A., DiDone, L., and Krysan, D. J., Extracellular Secretion of Overexpressed Glycosylphosphatidylinositol-Linked Cell Wall Protein Utr2/Crh2p as a Novel Protein Quality Control Mechanism in Saccharomyces cerevisiae, Eukaryot. Cell, 2010, vol. 9, pp. 1669-1679.
17. Bourbonnais, Y., Larouche, C., and Tremblay, G. M., Production of Full-Length Human Pre-Elafin, an Elastase Specific Inhibitor, from Yeast Requires the Absence of a Functional Yapsin 1 (Yps1p) Endoprotease, Protein Expres. Purif., 2000, vol. 20, pp. 485-491.
18. Frieman, M. B. and Cormack, B. P., The Omega-Site Sequence of Glycosylphosphatidylinositol-Anchored Proteins in Saccharomyces cerevisiae Can Determine Distribution between the Membrane and the Cell Wall, Mol. Microbiol., 2003, vol. 50, pp. 883-896.
19. Sreekrishna, K., Potenz, R. H. B., Cruze, J. A., et al., High Level Expression of Heterologous Proteins in Methylotrophic Yeast Pichia pastoris, J. Basic. Microbiol., 1988, vol. 28, pp. 265-278.
20. Maniatis, T., Fritsch, E. F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1982.
21. Fujimura, H. and Sakuma, Y., Simplified Isolation of Chromosomal and Plasmid DNA from Yeasts, Bio-Techniques, 1993, vol. 14, pp. 538-540.
22. DNA, Cloning: A Practical Approach, Glover, D., Ed., Oxford: IRL Press, 1985, pp. 291-402.
23. Al-Maghrebi, M., Brule, H., Padkina, M., et al., The 3′ Untranslated Region of Human Vimentin mRNA Interacts with Protein Complexes Containing eEF-1gamma and HAX-1, Nucleic Acids Res., 2002, vol. 30, pp. 5017-5028.
24. Krysan, D.-J., Ting, E., Abeijon, C., et al., Yapsins Are a Family of Aspartyl Proteases Required for Cell Wall Integrity in Saccharomyces cerevisiae, Eukaryot. Cell, 2005, vol. 4, pp. 1364-1374.
25. Gagnon-Arsenault, I., Parise, L., Tremblay, J., and Bourbonnais, Y., Activation Mechanism, Functional Role and Shedding of Glycosylphosphatidylinositol-Anchored Yps1p at the Saccharomyces cerevisiae Cell Surface, Mol. Microbiol., 2008, vol. 69, pp. 982-993.
26. Padkina, M. V., Parfenova, L. V., Sambuk, E. V., et al., RF Patent 2203950, 2002.
27. Cregg, J. M. and Madden, K. R., Development of the Methylotrophic Yeast, Pichia pastoris, as a Host System for the Production of Foreign Proteins, Dev. Ind. Microbiol. (J. Ind. Microbiol.), 1988, vol. 29, suppl. 3, pp. 33-41.
28. Padkina, M. V., Parfenova, L. V., Gradoboeva, A. E., and Sambuk, E. V., Heterologous Interferons Synthesis in Yeast Pichia pastoris, Prikl. Biokhim. Mikrobiol., 2010, vol. 46, no. 4, pp. 448-455.