Degradation of HSA-AX15(R13K) when expressed in Pichia pastoris can be reduced via the disruption of YPS1 gene in this yeast

Journal of Biotechnology

Volumn 139, Issue 2, 2009, Pages 131-136

  Yao, Xue Qin ^a   Zhao, Hong Liang ^a   Xue, Chong ^a   Zhang, Wei ^a   Xiong, Xiang Hua ^a   Wang, Zhi Wei ^a   Li, Xian Yu ^a   Liu, Zhi Min ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Gene disruption; HSA AX15(R13K); Pichia pastoris; Protein degradation; YPS1 gene

Indexed keywords

GENE DISRUPTION; HSA-AX15(R13K); PICHIA PASTORIS; PROTEIN DEGRADATION; YPS1 GENE;

CELL CULTURE; CELL GROWTH; GROWTH (MATERIALS); GROWTH KINETICS; PROTEINS; STRAIN;

DEGRADATION;

ARGININE; ASPARTIC PROTEINASE; LYSINE; PROTEIN HSA AX15; PROTEIN YPS1; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CELL GROWTH; CONTROLLED STUDY; FUNGAL GENE; FUNGAL STRAIN; GENE CONSTRUCT; GENE DISRUPTION; HOMOLOGOUS RECOMBINATION; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; SUPERNATANT; WESTERN BLOTTING; WILD TYPE;

ASPARTIC ENDOPEPTIDASES; BLOTTING, WESTERN; CELL PROLIFERATION; CILIARY NEUROTROPHIC FACTOR; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FUNGAL PROTEINS; HUMANS; MUTANT PROTEINS; MUTATION; PICHIA; RECOMBINANT FUSION PROTEINS;

PICHIA PASTORIS;

EID: 57949087782     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.09.006     Document Type: Article

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