A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells

Nature Communications

Volumn 4, Issue , 2013, Pages

  Zhang, Rong ^a,f   Mjoseng, Heidi K ^b   Hoeve, Marieke A ^b   Bauer, Nina G ^b   Pells, Steve ^b   Besseling, Rut ^c   Velugotla, Srinivas ^d   Tourniaire, Guilhem ^a   Kishen, Ria E B ^b   Tsenkina, Yanina ^b   Armit, Chris ^b   Duffy, Cairnan R E ^b   Helfen, Martina ^e   Edenhofer, Frank ^e   De Sousa, Paul A ^b   Bradley, Mark ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c NV ORGANON   (Netherlands)

^d UNIVERSITY OF EDINBURGH   (United Kingdom)

^e UNIVERSITY OF BONN   (Germany)

^f CHANGZHOU UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLIC ACID; COLLAGEN; LAMININ; MATRIGEL; PROTEOGLYCAN;

CELL ORGANELLE; EMBRYONIC DEVELOPMENT; GEL; GROWTH; TEMPERATURE EFFECT;

ARTICLE; CELL GROWTH; CHEMICAL COMPOSITION; CONTROLLED STUDY; EMBRYONIC STEM CELL; ENVIRONMENTAL TEMPERATURE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HYDROGEL; MEDICAL RESEARCH; MODULATION; PLURIPOTENT STEM CELL; SHEAR STRESS; STEM CELL CULTURE; THERMAL ANALYSIS; BIOPHYSICS; CELL ADHESION; CELL CULTURE; CHEMISTRY; CLONOGENIC ASSAY; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; DRUG COMBINATION; DRUG EFFECT; MECHANICAL STRESS; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE; TIME;

BIOPHYSICAL PHENOMENA; CELL ADHESION; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COLLAGEN; COLONY-FORMING UNITS ASSAY; CULTURE MEDIA; DRUG COMBINATIONS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EMBRYONIC STEM CELLS; HUMANS; HYDROGEL; LAMININ; PROTEOGLYCANS; STRESS, MECHANICAL; TEMPERATURE; TIME FACTORS;

MLCS; MLOWN;

EID: 84879131924     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms2341     Document Type: Article

References (43)

1. Li, Y., Powell, S., Brunette, E., Lebkowski, J. & Mandalam, R. Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products. Biotechnol. Bioeng. 91, 688-698 (2005). (Pubitemid 41283861)
2. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185-187 (2006). (Pubitemid 43221701)
3. Wang, L. et al. Self-renewal of human embryonic stem cells requires insulinlike growth factor-1 receptor and ERBB2 receptor signaling. Blood 110, 4111-4119 (2007). (Pubitemid 350248470)
4. Watanabe, K. et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25, 681-686 (2007). (Pubitemid 46897207)
5. Xu, C. et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat. Biotechnol. 19, 971-974 (2001). (Pubitemid 32947578)
6. Yao, S. et al. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl Acad. Sci. USA 103, 6907-6912 (2006).
7. Oh, S. K. et al. Long-term microcarrier suspension cultures of human embryonic stem cells. Stem Cell Res. 2, 219-230 (2009).
8. Steiner, D. et al. Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat. Biotechnol. 28, 361-364 (2010).
9. Braam, S. R. et al. Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin. Stem Cells 26, 2257-2265 (2008).
10. Liu, Y. et al. A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells. Biochem. Biophys. Res. Commun. 346, 131-139 (2006). (Pubitemid 43865977)
11. Rodin, S. et al. Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511. Nat. Biotechnol. 28, 611-615 (2010).
12. Anderson, D. G., Levenberg, S. & Langer, R. Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells. Nat. Biotechnol. 22, 863-866 (2004). (Pubitemid 38886605)
13. Mei, Y. et al. Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. Nat. Mater. 9, 768-778 (2010).
14. Melkoumian, Z. et al. Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells. Nat. Biotechnol. 28, 606-610 (2010).
15. Villa-Diaz, L. G. et al. Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nat. Biotechnol. 28, 581-583 (2010).
16. Li, Y. J., Chung, E. H., Rodriguez, R. T., Firpo, M. T. & Healy, K. E. Hydrogels as artificial matrices for human embryonic stem cell self-renewal. J. Biomed. Mater. Res. 79A, 1-5 (2006). (Pubitemid 44452848)
17. Chen, G. et al. Chemically defined conditions for human iPSC derivation and culture. Nat. Methods 8, 424-429 (2011).
18. Baran, E. J. Chelation therapies: a chemical and biochemical perspective. Curr. Med. Chem. 17, 3658-3672 (2010).
19. Khalil, W. K. et al. The inhibitory effects of garlic and Panax ginseng extract standardized with ginsenoside Rg3 on the genotoxicity, biochemical, and histological changes induced by ethylenediaminetetraacetic acid in male rats. Arch. Toxicol. 82, 183-195 (2008). (Pubitemid 351502741)
20. da Silva, R. M., Mano, J. F. & Reis, R. L. Smart thermoresponsive coatings and surfaces for tissue engineering: switching cell-material boundaries. Trends Biotechnol. 25, 577-583 (2007). (Pubitemid 350148217)
21. Ohashi, K. et al. Engineering functional two-and three-dimensional liver systems in vivo using hepatic tissue sheets. Nat. Med. 13, 880-885 (2007). (Pubitemid 47038184)
22. Wolff, J. A. & Rozema, D. B. Breaking the bonds: non-viral vectors become chemically dynamic. Mol. Ther. 16, 8-15 (2008).
23. Oya, T. et al. Reversible molecular adsorption based on multiple-point interaction by shrinkable gels. Science 286, 1543-1545 (1999).
24. Zhang, R., Liberski, A., Khan, F., Diaz-Mochon, J. J. & Bradley, M. Inkjet fabrication of hydrogel microarrays using in situ nanolitre-scale polymerisation. Chem. Commun. (Camb) 11, 1317-1319 (2008). (Pubitemid 351490513)
25. Zhang, R., Liberski, A., Sanchez-Martin, R. & Bradley, M. Microarrays of over 2000 hydrogels-identification of substrates for cellular trapping and thermally triggered release. Biomaterials 30, 6193-6201 (2009).
26. Pernagallo, S., Diaz-Mochon, J. J. & Bradley, M. A cooperative polymer-DNA microarray approach to biomaterial investigation. Lab. Chip. 9, 397-403 (2009).
27. Pethig, R., Menachery, A., Pells, S. & De Sousa, P. Dielectrophoresis: a review of applications for stem cell research. J. Biomed. Biotechnol. 2010, 182581 (2010).
28. Khan, F., Tare, R. S., Oreffo, R. O. & Bradley, M. Versatile biocompatible polymer hydrogels: scaffolds for cell growth. Angew. Chem. Int. Ed. Engl. 48, 978-982 (2009).
29. Amps, K. et al. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nat. Biotechnol. 29, 1132-1144 (2011).
30. Caisander, G. et al. Chromosomal integrity maintained in five human embryonic stem cell lines after prolonged in vitro culture. Chromosome Res. 14, 131-137 (2006).
31. Narva, E. et al. High-resolution DNA analysis of human embryonic stem cell lines reveals culture-induced copy number changes and loss of heterozygosity. Nat. Biotechnol. 28, 371-377 (2010).
32. Higuchi, A., Ling, Q. D., Ko, Y. A., Chang, Y. & Umezawa, A. Biomaterials for the feeder-free culture of human embryonic stem cells and induced pluripotent stem cells. Chem. Rev. 111, 3021-3035 (2011).
33. Kleinman, H. K. & Martin, G. R. Matrigel: basement membrane matrix with biological activity. Semin. Cancer Biol. 15, 378-386 (2005).
34. Baker D. E. et al. Adaptation to culture of human embryonic stem cells and oncogenesis in vivo. Nat. Biotechnol. 25, 207-215 (2007). (Pubitemid 46227120)
35. Stephenson, E. et al. Safety paradigm: genetic evaluation of therapeutic grade human embryonic stem cells. J. R. Soc. Interface 7(Suppl 6): S677-S688 (2010).
36. Loh, X. J. et al. Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells. Macromol. Biosci. 9, 1069-1079 (2009).
37. Kang, I. K., Lee, D. W., Lee, S. K. & Akaike, T. Grafting of lactose-carrying styrene onto polystrene dishes using plasma glow discharge and their interaction with hepatocytes. J. Mater. Sci. Mater. Med. 14, 611-616 (2003). (Pubitemid 36851212)
38. Redon, R. et al. Global variation in copy number in the human genome. Nature 444, 444-454 (2006). (Pubitemid 44809057)
39. De Sousa, P. A. et al. Clinically failed eggs as a source of normal human embryo stem cells. Stem Cell Res. 2, 188-197 (2009).
40. Fletcher, J. M. et al. Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines. Cloning Stem Cells 8, 319-334 (2006). (Pubitemid 46146039)
41. Thomson, J. A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147 (1998). (Pubitemid 28516247)
42. Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods 25, 402-408 (2001). (Pubitemid 34164012)
43. Klim, J. R., Li, L., Wrighton, P. J., Piekarczyk, M. S. & Kiessling, L. L. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods 7, 989-994 (2010).