Selection of endogenous reference genes for gene expression analysis in the mediterranean species of the Bemisia tabaci (Hemiptera: Aleyrodidae) complex

Journal of Economic Entomology

Volumn 106, Issue 3, 2013, Pages 1446-1455

  Su, Yun Lin ^a   He, Wen Bo ^a   Wang, Jia ^b   Li, Jun Min ^c   Liu, Shu Sheng ^a   Wang, Xiao Wei ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b SOUTHWEST UNIVERSITY   (China)

^c INSTITUTE OF PLANT PROTECTION AND MICROBIOLOGY   (China)

Author keywords

Bemisia tabaci; Endogenous reference gene; Gene expression; Quantitative PCR; RNA seq

Indexed keywords

INSECTICIDE;

ANIMAL; ARTICLE; BIOLOGICAL PEST CONTROL; DRUG EFFECT; ESSENTIAL GENE; FEMALE; GENE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HEMIPTERA; INSECT; INSECT CONTROL; MALE; MICROBIOLOGY; NYMPH; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

ANIMALS; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENES, ESSENTIAL; GENES, INSECT; HEMIPTERA; INSECT CONTROL; INSECTICIDES; MALE; NYMPH; PEST CONTROL, BIOLOGICAL; PUPA; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, RNA;

ALEYRODIDAE; BACTERIA (MICROORGANISMS); BEMISIA TABACI; HEMIPTERA;

EID: 84878821176     PISSN: 00220493     EISSN: None     Source Type: Journal    
DOI: 10.1603/EC12459     Document Type: Article

References (48)

1. Alon, M., M. Elbaz, M. M. Ben-Zvi, E. Feldmesser, A. Vainstein, and S. Morin. 2012. Insights into the transcriptomics of polyphagy: Bemisia tabaci adaptability to phenylpropanoids involves coordinated expression of defense and metabolic genes. Insect Biochem. Mol. Biol. 42: 251-263.
2. Andersen, C. L., J. L. Jensen, and T. F. Ørntoft. 2004. Normalization of real-time quantitative reverse transcription-PCRdata: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64: 5245-5250.
3. Bansal, R., P. Mamidala, M.A.R. Mian, O. Mittapalli, and A. P. Michel. 2012. Validation of reference genes for gene expression studies in Aphis glycines (Hemiptera: Aphididae). J. Econ. Entomol. 105: 1432-1438.
4. Berdiales, B., J. J. Bernal, E. Sáez, B. Woudt, F. Beitia, and E. Rodríguez-Cerezo. 1999. Occurrence of cucurbit yellow stunting disorder virus (CYSDV) and beet pseudoyellows virus in cucurbit crops in Spain and transmission of CYSDV by two biotypes of Bemisia tabaci. Phytopathology 105: 211-215.
5. Bustin, S. 2002. Quanti-cation of mRNA using real-time reverse transcriptionPCR(RT-PCR): Trends and problems. J. Mol. Endocrinol. 29: 23-39.
6. Cheng,W.C., C.W.Chang, C. R. Chen, M. L. Tsai,W.Y. Shu, C. Y. Li, and I. C. Hsu. 2011. Identi-cation of reference genes across physiological states for qRT-PCR through microarray meta-analysis. PLoS One 6: E17347.
7. De Barro, P., and F. Driver. 1997. Use of RAPD PCR to distinguish the B biotype from other biotypes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Aust. J. Entomol. 36: 149-152.
8. De Barro, P. J., S. S. Liu, L. M. Boykin, and A. B. Dinsdale. 2011. Bemisia tabaci: A statement of species status. Annu. Rev. Entomol. 56: 1-19.
9. DeBoer, M., T.DeBoer, J. Marie?n,M.Timmermans, B. Nota, N. Van Straalen, J. Ellers, and D. Roelofs. 2009. Reference genes for QRT-PCR tested under various stress conditions in Folsomia candida and Orchesella cincta (Insecta, Collembola). BMC Mol. Biol. 10: 54.
10. Elbaz, M., M. Weiser, and S. Morin. 2011. Asymmetry in thermal tolerance trade-offs between the B and Q sibling species of Bemisia tabaci (Hemiptera: Aleyrodidae). J. Evol. Biol. 24: 1099-1109.
11. Fernández, E., C. Grávalos, P. J. Haro, D. Cifuentes, and P. Bielza. 2009. Insecticide resistance status of Bemisia tabaci Q-biotype in south-astern Spain. Pest Manag. Sci. 65: 885-891.
12. Ginzinger, D. G. 2002. Gene quanti-cation using real-time quantitative PCR: An emerging technology hits the mainstream. Exp. Hematol. 30: 503-512.
13. Grabherr, M. G., B. J. Haas, M. Yassour, J. Z. Levin, D. A. Thompson, I. Amit, X. Adiconis, L. Fan, R. Raychowdhury, and Q. Zeng. 2011. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29: 644-652.
14. Guillermo, M., S. Mó nica, M. Vanesa, and T. Graciela. 2011. Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers. Virol. J. 8: 308.
15. Horowitz, A. R., S. Kontsedalov, V. Khasdan, and I. Ishaaya. 2005. Biotypes B and Q of Bemisia tabaci and their relevance to neonicotinoid and pyriproxyfen resistance. Arch. Insect Biochem. Physiol. 58: 216-225.
16. Hu, J., P. De Barro, H. Zhao, J. Wang, F. Nardi, and S. S. Liu. 2011. An extensive field survey combined with a phylogenetic analysis reveals rapid and widespread invasion of two alien whiteßies in China. PLoS One 6: E16061.
17. Huggett, J., K. Dheda, S. Bustin, and A. Zumla. 2005. Realtime RT-PCR normalisation; strategies and considerations. Genes Immunol. 6: 279-284.
18. Jones, C. M., M. Daniels, M. Andrews, R. Slater, R. J. Lind, K. Gorman, M. S. Williamson, and I. Denholm. 2008. Age-speci-c expression of a P450 monooxygenase (CYP6CM1) correlates with neonicotinoid resistance in Bemisia tabaci. Pestic. Biochem. Physiol. 101: 53-58.
19. Karunker, I., J. Benting, B. Lueke, T. Ponge, R. Nauen, E. Roditakis, J. Vontas, K. Gorman, I. Denholm, and S. Morin. 2008. Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochem. Mol. Biol. 38: 634-644.
20. Li, M., J. Hu, F. C. Xu, and S. S. Liu. 2010. Transmission of Tomato yellow leaf curl virus by two invasive biotypes and a Chinese indigenous biotype of the whiteßy Bemisia tabaci. Int. J. Pest Manage. 56: 275-280.
21. Li, J. M., Y. L. Su, X. L. Gao, J. He, S. S. Liu, and X. W. Wang. 2011. Molecular characterization, and oxidative stress response of an intracellular Cu/Zn superoxide dismutase (CuZnSOD) of the whiteßy, Bemisia tabaci. Arch. Insect Biochem. Physiol. 77: 118-133.
22. Li, R. M., W. Xie, S. L. Wang, Q. J. Wu, N. N. Yang, X. Yang, H. P. Pan, X. M. Zhou, L. Y. Bai, B. Y. Xu et al. 2013. Reference gene selection for qRT-PCR analysis in the sweetpotato whiteßy, Bemisia tabaci (Hemiptera: Aleyrodidae). PLoS One 8: E53006.
23. Ling, D., and P. M. Salvaterra. 2011. Robust RT-qPCR data normalization: Validation and selection of internal reference genes during post-experimental data analysis. PLoS One 6: E17762.
24. Liu, S. S., J. Colvin, and P. J. De Barro. 2012. Species concepts as applied to the whiteßy Bemisia tabaci systematics: How many species are there? J. Integr. Agric. 11: 176-186.
25. Lord, J. C., K. Hartzer, M. Toutges, and B. Oppert. 2010. Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge. J. Microbiol. Methods 80: 219-221.
26. Lourenço, A. P., A. Mackert, A. S. Cristino, and Z.L.P. Simöes. 2008. Validation of reference genes for gene expression studies in the honey bee, Apis mellifera, by quantitative real-time RT-PCR. Apidologie 39: 372-385.
27. Luan, J. B., J. M. Li, N. Varela, Y. L. Wang, F. F. Li, Y. Y. Bao, C. X. Zhang, S. S. Liu, and X. W. Wang. 2011. Global analysis of the transcriptional response of whiteßy to Tomato yellow leaf curl China virus reveals the relationship of coevolved adaptations. J. Virol. 85: 3330-3340.
28. Mahadav, A., D. Gerling, Y. Gottlieb, H. Czosnek, and M. Ghanim. 2008. Parasitization by the wasp Eretmocerus mundus induces transcription of genes related to immune response and symbiotic bacteria proliferation in the whiteßy Bemisia tabaci. BMC Genomics 9: 342.
29. Mahadav, A., S. Kontsedalov, H. Czosnek, and M. Ghanim. 2009. Thermotolerance and gene expression following heat stress in the whiteßy Bemisia tabaci B and Q biotypes. Insect Biochem. Mol. Biol. 39: 668-676.
30. Mamidala, P., S. P. Rajarapu, S. C. Jones, and O. Mittapalli. 2011. Identi-cation and validation of reference genes for quantitative real-time polymerase chain reaction in Cimex lectularius. J. Med. Entomol. 48: 947-951.
31. Nauen, R., N. Stumpf, and A. Elbert. 2002. Toxicological and mechanistic studies on neonicotinoid cross resistance in Q-type Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag. Sci. 58: 868-875.
32. Paim, R. M., M. H. Pereira, R. Di Ponzio, J. O. Rodrigues, A. A. Guarneri, N. F. Gontijo, and R. N. Araú jo. 2012. Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real-time PCR. BMC Res. Notes 5: 128.
33. Radonic, A., S. Thulke, I. M. Mackay, O. Landt, W. Siegert, and A. Nitsche. 2004. Guideline to reference gene selection for quantitative real-time PCR. Biochem. Biophys. Res. Commun. 313: 856-862.
34. Rauch, N., and R. Nauen. 2004. Characterization and molecular cloning of a glutathione S-transferase from the whiteßy Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochem. Mol. Biol. 34: 321-329.
35. Roditakis, E., E. Morou, A. Tsagkarakou, M. Riga, R. Nauen, M. Paine, S. Morin, and J. Vontas. 2011. Assessment of the Bemisia tabaci CYP6CM1vQ transcript and protein levels in laboratory and -eld-derived imidacloprid-resistant insects and cross-metabolism potential of the recombinant enzyme. Insect Sci. 18: 23-29.
36. Sánchez-Campos, S., J. Navas-Castillo, R. Camero, C. Soria, J. Diaz, and E. Moriones. 1999. Displacement of tomato yellow leaf curl virus (TYLCV)-Sr by TYLCV-Is in tomato epidemics in Spain. Phytopathology 89: 1038-1043.
37. Scharlaken, B., D. C. de Graaf, K. Goossens, M. Brunain, L. J. Peelman, and F. J. Jacobs. 2008. Reference gene selection for insect expression studies using quantitative realtime PCR: The head of the honeybee, Apis mellifera, after a bacterial challenge. J. Insect Sci. 8: 1-10.
38. Shen, G. M., H. B. Jiang, X. N. Wang, and J. J. Wang. 2010. Evaluation of endogenous references for gene expression pro-ling in different tissues of the oriental fruit ßy Bactrocera dorsalis (Diptera: Tephritidae). BMC Mol. Biol. 11: 76.
39. Sinisterra, X. H., C. McKenzie, W. B. Hunter, C. A. Powell, and R. G. Shatters Jr. 2005. Differential transcriptional activity of plant-pathogenic begomoviruses in their whiteßy vector (Bemisia tabaci, Gennadius: Hemiptera Aleyrodidae). J. Gen. Virol. 86: 1525-1532.
40. Spanier, K. I., F. Leese, C. Mayer, J. K. Colbourne, D. Gilbert, M. E. Pfrender, and R. Tollrian. 2010. Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR. BMC Mol. Biol. 11: 50.
41. Su, Y. L., J. M. Li, M. Li, J. B. Luan, X. D. Ye, X. W. Wang, and S. S. Liu. 2012. Transcriptomic analysis of the salivary glands of an invasive whiteßy. PLoS One 7: E39303.
42. Sun, W., Y. Jin, L. He, W. C. Lu, and M. Li. 2010. Suitable reference gene selection for different strains and developmental stages of the Carmine Spider Mite, Tetranychus cinnabarinus, using quantitative real-time PCR. J. Insect Sci. 10: 1-12.
43. Suzuki, T., P. Higgins, and D. Crawford. 2000. Control selection for RNA quantitation. Biotechniques 29: 332-337.
44. Van Hiel, M. B., P. Van Wielendaele, L. Temmerman, S. Van Soest, K. Vuerinckx, R. Huybrechts, J. V. Broeck, and . Simonet. 2009. Identi-cation and validation of housekeeping genes in brains of the desert locust Schistocerca gregaria under different developmental conditions. BMC Mol. Biol. 10: 56.
45. Vandesompele, J., K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, and F. Speleman. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3: Research0034.
46. Wang, X. W., J. B. Luan, J. M. Li, Y. Y. Bao, C. X. Zhang, and S. S. Liu. 2010. De novo characterization of a whiteßy transcriptome and analysis of its gene expression during development. BMC Genomics 11: 400.
47. Wang, X. W., J. B. Luan, J. M. Li, Y. L. Su, J. Xia and S. S Liu. 2011. Transcriptome analysis and comparison reveal divergence between two invasive whiteßy cryptic species. BMC Genomics 12: 458.
48. Wang, X. W., Q. Y. Zhao, J. B. Luan, Y. J. Wang, G. H. Yan, and S. S. Liu. 2012. Analysis of a native whiteßy transcriptome and its sequence divergence with two invasive whiteßy species. BMC Genomics 13: 529.