Reference Gene Selection for qRT-PCR Analysis in the Sweetpotato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Li, Rumei ^a,b   Xie, Wen ^b   Wang, Shaoli ^b   Wu, Qingjun ^b   Yang, Nina ^b   Yang, Xin ^b   Pan, Huipeng ^b,c   Zhou, Xiaomao ^a   Bai, Lianyang ^a   Xu, Baoyun ^b   Zhou, Xuguo ^c   Zhang, Youjun ^a,b  

^a HUNAN AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c UNIVERSITY OF KENTUCKY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOPHILIN A; ELONGATION FACTOR 1ALPHA; HEAT SHOCK PROTEIN 40; INSECTICIDE; PROTEIN L29; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; RIBOSOME PROTEIN; SUCCINATE DEHYDROGENASE COMPLEX SUBUNIT A; UNCLASSIFIED DRUG;

ABIOTIC STRESS; ANIMAL TISSUE; ARTICLE; BIOTIC STRESS; BIOTYPE; BODY REGIONS; COMPUTER PROGRAM; CONTROLLED STUDY; DEVELOPMENTAL STAGE; GENE EXPRESSION; GENETIC ALGORITHM; GENETIC SELECTION; HEMIPTERA; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTOPERIODICITY; PLANT VIRUS; PROTEIN STABILITY; REAL TIME POLYMERASE CHAIN REACTION; REFERENCE GENE SELECTION; SWEET POTATO WHITEFLY; TEMPERATURE; ANIMAL; BRASSICA; CUCUMBER; GENE; GENE EXPRESSION PROFILING; GENETICS; METHODOLOGY; PARASITOLOGY; TOMATO; VIROLOGY;

ALEYRODIDAE; BEMISIA TABACI; HEMIPTERA; HEXAPODA;

ANIMALS; BRASSICA; CUCUMIS SATIVUS; GENE EXPRESSION PROFILING; GENES, INSECT; HEMIPTERA; LYCOPERSICON ESCULENTUM; REAL-TIME POLYMERASE CHAIN REACTION;

MLCS; MLOWN;

EID: 84872191172     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0053006     Document Type: Article

References (53)

1. Ginzinger DG, (2002) Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30: 503-512.
2. Bustin SA, Benes V, Nolan T, Pfaffl MV, (2005) Quantitative real-time RT-PCR a perspective. J Mol Endocrinol 34: 597-601.
3. Kubista M, Andrade JM, Bengtsson M, Forootan M, Jonák J, et al. (2006) The real-time polymerase chain reaction. Mol Aspects Med 27: 95-125.
4. Van Guilder HD, Vrana KE, Freeman WM, (2008) Twenty-five years of quantitative PCR for gene expression analysis. Biotech 44: 619-626.
5. Thellin O, Zorzi W, Lakaye B, Coumans B, Hennen G, et al. (1999) Housekeeping genes as internal standards: use and limits. J Biotech 75: 291-295.
6. Suzuki T, Higgins PJ, Crawford DR, (2000) Control selection for RNA quantization. Biotech 29: 332-337.
7. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP, (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotech Lett 26: 509-515.
8. Tunbridge EM, Eastwood SL, Harrison PJ, (2011) Changed relative to what? housekeeping genes and normalization strategies human brain gene expression studies. Biol Psychiatry 69: 173-179.
9. Radonić A, Thulke S, Mackay IM, Landt O, Siegert W, et al. (2004) Guideline to reference gene selection for quantitative real-time PCR. Biochem Bioph Res Co 313: 856-862.
10. Vandesompele J, De Preter K, Pattyn F, Poppe B, Roy NV, et al. (2002) Accurate normalization of realtime quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: 1-12.
11. Ponton F, Chapuis MP, Pernice M, Sword GA, Simpson SJ, (2011) Evaluation of potential reference genes for reverse transcription qPCR studies of physiological responses in Drosophila melanogaster. J Insect Physiol 57: 841.
12. Zhou XG, Tarver MR, Bennett GW, Oi FM, Scharf ME, (2006) Two hexamerin genes from the termite Reticulitermes flavipes: sequence, expression, and proposed functions in caste regulation. Gene 376: 47-58.
13. Andersen CL, Jensen JL, Ørntoft TF, (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64: 5245-5250.
14. Brown JK, Frohlich DR, Rosell RC, (1995) The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annu Rev Entomol 40: 511-534.
15. Inbar M, Gerling D, (2008) Plant-mediated interactions between whiteflies, herbivores and natural enemies. Ann Rev Entomol 53: 431-448.
16. Jones DR, (2003) Plant viruses transmitted by whiteflies. Eu J Plant Pathol 109: 195-219.
17. Perring TM, (2001) The Bemisia tabaci species complex. Crop Prot 20: 725-737.
18. Dinsdale A, Cook L, Riginos C, Buckley YM, De Barro PJ, (2010) Refined global analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidae) mitochondrial cytochrome oxidase 1 to identify species level genetic boundaries. Ann Entomol Soc Am 103: 196-208.
19. Xu J, De Barro PJ, Liu SS, (2010) Reproductive incompatibility among genetic groups of Bemisia tabaci supports the proposition that the whitefly is a cryptic species complex. Bull Entomol Res 100: 359-366.
20. De Barro PJ, Liu SS, Boykin LM, Dinsdale A, (2011) Bemisia tabaci: a statement of species status. Annu Rev Entomol 56: 1-19.
21. Luo C, Yao Y, Wang RJ, Yan FM, Hu DX, (2002) The use of mitochondrial cytochrome oxidase mtCO1 gene sequences for the identification of biotypes of Bemisia tabaci (Gennadius) in China. Acta Entomologica Sinica 4: 759-763.
22. Chu D, Zhang YJ, Brown JK, Cong B, Xu BY, et al. (2006) The introduction of the exotic biotype Q of Bemisia tabacifrom the Mediterranean region into China on ornamental crops. Fla Entomol 89: 168-174.
23. Pan HP, Chu D, Ge DQ, Wang SL, Wu QJ, et al. (2011) Further spread of and domination by Bemisia tabaci biotype Q on field crops in China. J Econ Entomol 104: 978-985.
24. Mahadav A, Kontsedalov S, Czosnek H, Ghanim M, (2009) Thermotolerance and gene expression following heat stress in the whitefly Bemisia tabaci B and Q biotypes. Insect Biochem Mol Biol 39: 668-676.
25. Ghanim M, Kontsedalov S, (2009) Susceptibility to insecticides in the Q biotype of Bemisia tabaci is correlated with bacterial symbiont densities. Pest Manag Sci 65: 939-942.
26. Upadhyay SK, Chandrashekar K, Thakur N, Verma PC, Borgio JF, et al. (2011) RNA interference for the control of whiteflies (Bemisia tabaci) by oral route. J Biosci 36: 153-161.
27. Lü ZC, Wan FH, (2011) Using double-stranded RNA to explore the role of heat shock protein genes in heat tolerance in Bemisia tabaci (Gennadius). J Exp Biol 214: 764-769.
28. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, et al. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55: 611-622.
29. Xie W, Meng QS, Wu QJ, Wang SL, Yang X, et al. (2012) Pyrosequencing the Bemisia tabaci transcriptome reveals a highly diverse bacterial community and a robust system for insecticide resistance. PLoS ONE 7: e35181.
30. Xie W, Wang SL, Wu QJ, Feng YT, Pan HP, et al. (2011) Induction effects of host plants on insecticide susceptibility and detoxification enzymes of Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag Sci 67: 87-93.
31. Wu JB, Dai FM, Zhou XP, (2006) First report of Tomato yellow leaf curl virus in China. Ann Appl Biol 155: 439-448.
32. Ghanim M, Morin S, Zeidan M, Czosnek M, (1998) Evidence for transovarial transmission of tomato yellow leaf curl virus by its vector, the whitefly Bemisia tabaci. Virology 240: 295-303.
33. Jiu M, Zhou XP, Tong L, Xu J, Yang X, et al. (2007) Vector-virus mutualism accelerates population increase of an invasive whitefly. PLoS ONE 2: 182.
34. Feng YT, Wu QJ, Wang SL, Chang XL, Xie W, et al. (2010) Cross-resistance study and biochemical mechanisms of thiamethoxam resistance in B-biotype Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag Sci 66: 313-318.
35. Willems E, Mateizel I, Kemp C, Cauffman G, Sermon K, et al. (2006) Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells. Int J Dev Biol 50: 627-635.
36. Hoogewijs D, Houthoofd K, Matthijssens F, Vandesompele J, Vanfleteren JR, (2008) Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans. BMC Mol Biol 9: 9.
37. Langnaese K, John R, Schweizer H, Ebmeyer U, Keilhoff G, (2008) Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model. BMC Mol Biol 9: 53.
38. Tatsumi K, Ohashi K, Taminishi S, Okano T, Yoshioka A, et al. (2008) Reference gene selection for real-time RT-PCR in regenerating mouse livers. Biochem Bioph Res Co 374: 106-110.
39. Huis R, Hawkins S, Neutelings G, (2010) Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.). BMC Plant Biol 10: 71.
40. Bustin SA, (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 29: 23-39.
41. Gutierrez L, Mauriat M, Guenin S, Pelloux J, Lefebvre JF, et al. (2008) The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. J Plant Biotech 6: 609-618.
42. Shen GM, Jiang HB, Wang XN, Wang JJ, (2010) Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae). BMC Mol Biol 11: 7.
43. Hornáková D, Matoušková P, Kindl J, Valterová I, Pichová I, (2010) Selection of reference genes for real-time polymerase chain reaction analysis in tissues from Bombus terrestris and Bombus lucorum of different ages. Anal Biochem 397: 118-120.
44. Jiang H, Liu Y, Tang P, Zhou A, Wang J, (2009) Validation of endogenous reference genes for insecticide-induced and developmental expression profiling of Liposcelis bostrychophila (Psocoptera: Liposcelididae). Molecular Biol Rep 37: 1019-1029.
45. Scharlaken B, de Graaf DC, Goossens K, Brunain M, Peelman LJ, et al. (2008) Reference gene selection for insect expression studies using quantitative real-time PCR: The head of the honeybee, Apis mellifera, after a bacterial challenge. J Insect Sci 8: 33.
46. Hiel MBV, Wielendaele PV, Temmerman L, Soest SV, Vuerinckx K, et al. (2009) Identification and validation of housekeeping genes in brains of the desert locust Schistocerca gregaria under different developmental conditions. BMC Mol Biol 10: 56.
47. Shen YM, Li Y, Ye F, Wang FF, Lu WG, et al. (2010) Identification of suitable reference genes for measurement of gene expression in human cervical tissues. Anal Biochem 405: 224-229.
48. Olsvik P, Lie K, Jordal AE, Nilsen T, Hordvik I, (2005) Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon. BMC Mol Bio l6: 21.
49. Silver N, Best S, Jiang J, Thein SL, (2006) Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol 7: 33.
50. Marie-Pierre C, Donya TE, Tim D, Laurence B, Fleur P, et al. (2011) Assessment and validation of a suite of reverse transcription-quantitative PCR reference genes for analyses of density-dependent behavioral plasticity in the Australian plague locust. BMC Mol Biol 12: 7.
51. Bustin SA, (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25: 169-193.
52. De Boer M, de Boer T, Marien J, Timmermans M, Nota B, et al. (2009) Reference genes for QRT-PCR tested under various stress conditions in Folsomia candida and Orchesella cincta (Insecta, Collembola). BMC Mol Biol 10: 5.
53. Kylee J, Veazey, Michael C, (2011) Golding selection of stable reference genes for quantitative RT-PCR comparisons of mouse embryonic and extra-embryonic stem cells. PLoS ONE 6: e27592.