Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR

Methods in Molecular Biology

Volumn 967, Issue , 2013, Pages 235-248

  Kolesnik, Tatiana B ^a   Nicholson, Sandra E ^a,b  

^a WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Ct value; Macrophages; Reference gene; Relative expression; RT quantitative PCR; SOCS; Standard curve; Suppressor of cytokine signalling

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; SUPPRESSOR OF CYTOKINE SIGNALING; INTERLEUKIN 4; PRIMER DNA;

AMPLICON; BONE MARROW DERIVED MACROPHAGE; CYTOKINE RESPONSE; DNA BASE COMPOSITION; GENE EXPRESSION; JAK-STAT SIGNALING; MRNA EXPRESSION LEVEL; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; SINGLE NUCLEOTIDE POLYMORPHISM; SYNTHESIS; UPREGULATION; ARTICLE; BIOSYNTHESIS; BONE MARROW CELL; CYTOLOGY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; HUMAN; MACROPHAGE; METABOLISM; METHODOLOGY; STANDARD;

BONE MARROW CELLS; DNA PRIMERS; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION; HUMANS; INTERLEUKIN-4; MACROPHAGES; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SUPPRESSOR OF CYTOKINE SIGNALING PROTEINS;

EID: 84878756789     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-242-1_17     Document Type: Chapter

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