Identification of Subunit-Subunit Interaction Sites in αA-WT Crystallin and Mutant αA-G98R Crystallin Using Isotope-Labeled Cross-Linker and Mass Spectrometry

PLoS ONE

Volumn 8, Issue 6, 2013, Pages

  Kannan, Rama ^a   Santhoshkumar, Puttur ^a   Mooney, Brian P ^a,b   Sharma, K Krishna ^a  

^a University of Missouri   (United States)

^b UNIVERSITY OF MISSOURI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CRYSTALLIN; ARGININE; GLYCINE; MUTANT PROTEIN; BIS(SULFOSUCCINIMIDYL)GLUTARATE; CROSS LINKING REAGENT; GLUTARIC ACID DERIVATIVE; PEPTIDE FRAGMENT; PROTEIN SUBUNIT; SUCCINIMIDE DERIVATIVE;

ARTICLE; BINDING SITE; CONTROLLED STUDY; ION EXCHANGE CHROMATOGRAPHY; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN CROSS LINKING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; WILD TYPE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN SECONDARY STRUCTURE; PROTEIN SUBUNIT; TANDEM MASS SPECTROMETRY;

ALPHA-CRYSTALLIN A CHAIN; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CHROMATOGRAPHY, ION EXCHANGE; CROSS-LINKING REAGENTS; GLUTARATES; HUMANS; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PEPTIDE FRAGMENTS; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN INTERACTION MAPPING; PROTEIN STRUCTURE, SECONDARY; PROTEIN SUBUNITS; SUCCINIMIDES; TANDEM MASS SPECTROMETRY;

EID: 84878746994     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0065610     Document Type: Article

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