Characterization of site-specific mutations in a short-chain-length/medium-chain-length polyhydroxyalkanoate synthase: In vivo and in vitro studies of enzymatic activity and substrate specificity

Applied and Environmental Microbiology

Volumn 79, Issue 12, 2013, Pages 3813-3821

  Chuah, Jo Ann ^a,c   Tomizawa, Satoshi ^a   Yamada, Miwa ^a   Tsuge, Takeharu ^b   Doi, Yoshiharu ^a   Sudesh, Kumar ^c   Numata, Keiji ^a  

^a RIKEN   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c SCHOOL OF PHYSICS   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMATIC ACTIVITIES; HYDROXYBUTYRATE; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOATES; SATURATION POINT; SHORT CHAIN LENGTHS; SUBSTRATE SPECIFICITY; SUBSTRATE-BINDING;

BIOCHEMISTRY; CESIUM; MONOMERS; ORGANIC POLYMERS; PLANTS (BOTANY);

SUBSTRATES;

ACYLTRANSFERASE; POLY(3-HYDROXYALKANOIC ACID) SYNTHASE; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOIC ACID;

CLASSIFICATION; CORRELATION; ENZYME ACTIVITY; MICROBIAL ACTIVITY; MUTATION; REACTION KINETICS; SUBSTRATE;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHROMOBACTERIUM; DNA SEQUENCE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; WESTERN BLOTTING;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, WESTERN; CHROMOBACTERIUM; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYHYDROXYALKANOATES; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

CHROMOBACTERIUM SP;

EID: 84878531495     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00564-13     Document Type: Article

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