Comparative and practical aspects of localization-based super-resolution imaging

Current Protocols in Cytometry

Volumn , Issue SUPPL.63, 2013, Pages

  Laevsky, Gary S ^a   O'Connell, Christopher B ^a  

^a Nikon USA   (United States)

Author keywords

PALM; STORM; Super resolution microscopy; Tandem dyes

Indexed keywords

FLUORESCENT DYE; PHOTOACTIVATABLE PROTEIN; PHOTOSWITCHABLE PROTEIN; PROTEIN; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CELL LABELING; CELL STRUCTURE; CLINICAL PROTOCOL; COMPARATIVE STUDY; CONTROLLED STUDY; DIFFRACTION; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; IMAGE PROCESSING; IMAGE QUALITY; INTERMETHOD COMPARISON; MICROSCOPY; PRIORITY JOURNAL; SUPER RESOLUTION MICROSCOPY; ANIMAL; CHEMISTRY; EQUIPMENT; HUMAN; IMAGE CYTOMETRY; IMAGE ENHANCEMENT; METHODOLOGY; MICROTUBULE; MITOCHONDRION; PROFESSIONAL PRACTICE; STAINING; ULTRASTRUCTURE;

ANIMALS; FLUORESCENT DYES; HUMANS; IMAGE CYTOMETRY; IMAGE ENHANCEMENT; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY; MICROSCOPY, FLUORESCENCE; MICROTUBULES; MITOCHONDRIA; PROFESSIONAL PRACTICE; STAINING AND LABELING;

MLCS; MLOWN;

EID: 84878513754     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy0220s63     Document Type: Article

References (15)

1. Annibale, P., Scarselli, M.,Kodiyan, A., and Radenovic, A. 2010. Photoactivatable fluorescent protein mEos2 displays repeated photoactivation after a long-lived dark state in the red photoconverted form. J. Phys. Chem. Lett. 1:1506-1510.
2. Bates, M., Blosser, T.R., and Zhuang, X. 2005. Short-range spectroscopic ruler based on a single-molecule optical switch. Phys. Rev. Lett. 94:108101.
3. Bates, M., Huang, B., Dempsey, G.T., and Zhuang, X. 2007. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science 317:1749-1753.
4. Betzig, E., Patterson, G.H., Sougrat, R., Lindwasser, O.W., Olenych, S., Bonifacino, J.S., Davidson, M.W., Lippincott-Schwartz, J., and Hess, H.F. 2006. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313:1642-1645.
5. Chudakov, D.M., Matz, M.V., Lukyanov, S., and Lukyanov, K.A. 2010. Fluorescent proteins and their applications in imaging living cells and tissues. Physiol. Rev. 90:1103-1163.
6. Dempsey, G.T., Bates, M., Kowtoniuk, W.E., Liu, D.R., Tsien, R.Y., and Zhuang, X. 2009. Photoswitching mechanism of cyanine dyes. J. Am. Chem. Soc. 131:18192-18193.
7. Dempsey, G.T., Vaughan, J.C., Chen, K.H., Bates, M., and Zhuang, X. 2011. Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging. Nat. Methods 8:1027-1036.
8. Huang, B., Wang, W., Bates, M., and Zhuang, X. 2008. Three-dimensional super-resolution imaging by stochastic optical reconstructionmicroscopy. Science 319:810-813.
9. Jones, S.A., Shim, S.-H., He, J., and Zhuang, X. 2011. Fast, three-dimensional super-resolution imaging of live cells. Nat. Methods 8:499-508.
10. Patterson, G.H. 2011. Highlights of the optical highlighter fluorescent proteins. J. Microsc. 243:1-7.
11. Rust, M.J., Bates, M., and Zhuang, X. 2006. Subdiffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3:793-795.
12. Shroff,H., Galbraith,C.G., Galbraith, J.A., andBetzig, E. 2008. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat. Methods 5:417-423.
13. van de Linde, S., Löschberger, A., Klein, T., Heidbreder, M., Wolter, S., Heilemann, M., and Sauer, M. 2011. Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat. Protocols 6:991-1009.
14. Veatch, S.L., Machta, B.B., Shelby, S.A., Chiang, E.N., Holowka, D.A., and Baird, B.A. 2012. Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting. PloS One 7:e31457.
15. Yildiz, A., Forkey, J.N., McKinney, S.A., Ha, T., Goldman,Y.E., and Selvin, P.R. 2003. MyosinV walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science 300:2061-2065.