Brief demethylation step allows the conversion of adult human skin fibroblasts into insulin-secreting cells

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 22, 2013, Pages 8948-8953

  Pennarossa, Georgia ^a   Maffei, Sara ^a   Campagnol, Marino ^a   Tarantini, Letizia ^b,c   Gandolfi, Fulvio ^a   Brevini, Tiziana A L ^a  

^a Center for Stem Cell Research   (Italy)

^b Department of Clinical and Community Sciences ^*  (Italy)

^c UNIVERSITY OF MILAN   (Italy)

Author keywords

Cell Plasticity; Pancreatic Beta Cell

Indexed keywords

AZACITIDINE; GLUCOSE; HEPATOCYTE NUCLEAR FACTOR 1BETA; HEPATOCYTE NUCLEAR FACTOR 3BETA; HEPATOCYTE NUCLEAR FACTOR 4ALPHA; INSULIN; OCTAMER TRANSCRIPTION FACTOR 4; STREPTOZOCIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR ONECUT; TRANSCRIPTION FACTOR PDX 1; TRANSCRIPTION FACTOR REX1; TRANSCRIPTION FACTOR SOX17; TRANSCRIPTION FACTOR SOX2; UNCLASSIFIED DRUG;

ADULT; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CASE REPORT; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; DEMETHYLATION; FEMALE; GENE EXPRESSION; GLUCOSE BLOOD LEVEL; HUMAN; HUMAN CELL; MALE; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; SKIN FIBROBLAST; STREPTOZOCIN DIABETES;

CELL PLASTICITY; PANCREATIC BETA CELL;

ADULT; ANIMALS; AZACITIDINE; CELL TRANSDIFFERENTIATION; DNA METHYLATION; DNA MODIFICATION METHYLASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPIGENESIS, GENETIC; FIBROBLASTS; FLUORESCENT ANTIBODY TECHNIQUE; GLUCOSE TOLERANCE TEST; HUMANS; INDOLES; INSULIN; INSULIN-SECRETING CELLS; MICE; MICE, SCID; REGENERATIVE MEDICINE; SKIN;

MUS;

EID: 84878432544     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1220637110     Document Type: Article

References (47)

1. Lengner CJ (2010) iPS cell technology in regenerative medicine. Ann N Y Acad Sci 1192:38-44.
2. Cohen DE, Melton D (2011) Turning straw into gold: Directing cell fate for regenerative medicine. Nat Rev Genet 12(4):243-252.
3. Kiskinis E, Eggan K (2010) Progress toward the clinical application of patient-specific pluripotent stem cells. J Clin Invest 120(1):51-59.
4. Li W, Ding S (2010) Small molecules that modulate embryonic stem cell fate and somatic cell reprogramming. Trends Pharmacol Sci 31(1):36-45.
5. Ding S, Schultz PG (2004) A role for chemistry in stem cell biology. Nat Biotechnol 22(7):833-840.
6. Hemberger M, Dean W, ReikW(2009) Epigenetic dynamics of stem cells and cell lineage commitment: Digging Waddington's canal. Nat Rev Mol Cell Biol 10(8):526-537.
7. Zhou Q, Melton DA (2008) Extreme makeover: Converting one cell into another. Cell Stem Cell 3(4):382-388.
8. Li M, Liu G-H, Izpisua Belmonte JC (2012) Navigating the epigenetic landscape of pluripotent stem cells. Nat Rev Mol Cell Biol 13(8):524-535.
9. Plath K, Lowry WE (2011) Progress in understanding reprogramming to the induced pluripotent state. Nat Rev Genet 12(4):253-265.
10. Wu SC, Zhang Y (2010) Active DNA demethylation: Many roads lead to Rome. Nat Rev Mol Cell Biol 11(9):607-620.
11. Jones PA (1985) Effects of 5-azacytidine and its 2'-deoxyderivative on cell differentiation and DNA methylation. Pharmacol Ther 28(1):17-27.
12. Constantinides PG, Jones PA, Gevers W (1977) Functional striated muscle cells from non-myoblast precursors following 5-azacytidine treatment. Nature 267(5609):364-366.
13. Darmon M, Nicolas JF, Lamblin D (1984) 5-Azacytidine is able to induce the conversion of teratocarcinoma-derived mesenchymal cells into epithelia cells. EMBO J 3(5):961-967.
14. Brevini TA, et al. (2009) Cell lines derived from human parthenogenetic embryos can display aberrant centriole distribution and altered expression levels of mitotic spindle check-point transcripts. Stem Cell Rev 5(4):340-352.
15. Niwa H (2007) How is pluripotency determined and maintained? Development 134(4): 635-646.
16. Shi Y, et al. (2005) Inducing embryonic stem cells to differentiate into pancreatic beta cells by a novel three-step approach with activin A and all-trans retinoic acid. Stem Cells 23(5):656-662.
17. Wiese C, et al. (2004) Nestin expression-a property of multi-lineage progenitor cells? Cell Mol Life Sci 61(19-20):2510-2522.
18. D'Amour KA, et al. (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24(11):1392-1401.
19. Teramura Y, Iwata H (2010) Bioartificial pancreas microencapsulation and conformal coating of islet of Langerhans. Adv Drug Deliv Rev 62(7-8):827-840.
20. Ahlgren U, Pfaff SL, Jessell TM, Edlund T, Edlund H (1997) Independent requirement for ISL1 in formation of pancreatic mesenchyme and islet cells. Nature 385(6613):257-260.
21. Kroon E, et al. (2008) Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol 26(4):443-452.
22. Zhang H, et al. (2009) The LIM-homeodomain protein ISL1 activates insulin gene promoter directly through synergy with BETA2. J Mol Biol 392(3):566-577.
23. Lumelsky N, et al. (2001) Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets. Science 292(5520):1389-1394.
24. Xie R, et al. (2013) Dynamic chromatin remodeling mediated by polycomb proteins orchestrates pancreatic differentiation of human embryonic stem cells. Cell Stem Cell 12(2):224-237.
25. Boyd AW, Schrader JW (1982) Derivation of macrophage-like lines from the pre-B lymphoma ABLS 8.1 using 5-azacytidine. Nature 297(5868):691-693.
26. Taylor SM, Jones PA (1979) Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine. Cell 17(4):771-779.
27. Taylor SM, Jones PA (1982) Changes in phenotypic expression in embryonic and adult cells treated with 5-azacytidine. J Cell Physiol 111(2):187-194.
28. Chiu CP, Blau HM (1985) 5-Azacytidine permits gene activation in a previously noninducible cell type. Cell 40(2):417-424.
29. Enjoji M, Nakashima M, Honda M, Sakai H, Nawata H (1997) Hepatocytic phenotypes induced in sarcomatous cholangiocarcinoma cells treated with 5-azacytidine. Hepatology 26(2):288-294.
30. Blum B, et al. (2012) Functional beta-cell maturation is marked by an increased glucose threshold and by expression of urocortin 3. Nat Biotechnol 30(3):261-264.
31. Chen S, et al. (2009) A small molecule that directs differentiation of human ESCs into the pancreatic lineage. Nat Chem Biol 5(4):258-265.
32. Thatava T, et al. (2011) Indolactam V/GLP-1-mediated differentiation of human iPS cells into glucose-responsive insulin-secreting progeny. Gene Ther 18(3):283-293.
33. Bar-Nur O, Russ HA, Efrat S, Benvenisty N (2011) Epigenetic memory and preferential lineage-specific differentiation in induced pluripotent stem cells derived from human pancreatic islet beta cells. Cell Stem Cell 9(1):17-23.
34. Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA (2008) In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Nature 455(7213):627-632.
35. Banga A, Akinci E, Greder LV, Dutton JR, Slack JMW(2012) In vivo reprogramming of Sox9+ cells in the liver to insulin-secreting ducts. Proc Natl Acad Sci USA 109(38):15336-15341.
36. Mikkelsen TS, et al. (2008) Dissecting direct reprogramming through integrative genomic analysis. Nature 454(7200):49-55.
37. Stresemann C, Lyko F (2008) Modes of action of the DNA methyltransferase inhibitors azacytidine and decitabine. Int J Cancer 123(1):8-13.
38. Borowiak M, et al. (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4(4):348-358.
39. Jones PA, Taylor SM (1980) Cellular differentiation, cytidine analogs and DNA methylation. Cell 20(1):85-93.
40. Creusot F, Acs G, Christman JK (1982) Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidine and 5-aza-2'-deoxycytidine. J Biol Chem 257(4):2041-2048.
41. Haaf T (1995) The effects of 5-azacytidine and 5-azadeoxycytidine on chromosome structure and function: Implications for methylation-associated cellular processes. Pharmacol Ther 65(1):19-46.
42. Kahan BW, et al. (2003) Pancreatic precursors and differentiated islet cell types from murine embryonic stem cells: An in vitro model to study islet differentiation. Diabetes 52(8):2016-2024.
43. Andralojc KM, et al. (2009) Ghrelin-producing epsilon cells in the developing and adult human pancreas. Diabetologia 52(3):486-493.
44. Wierup N, Svensson H, Mulder H, Sundler F (2002) The ghrelin cell: A novel developmentally regulated islet cell in the human pancreas. Regul Pept 107(1-3):63-69.
45. Vignjevi-c S, et al. (2012) Similar developmental patterns of ghrelin-and glucagonexpressing cells in the human pancreas. Cells Tissues Organs 196(4):362-373.
46. Zerbini G, Podesta F, Meregalli G, Deferrari G, Pontremoli R (2001) Fibroblast Na+-Li+ countertransport rate is elevated in essential hypertension. J Hypertens 19(7):1263-1269.
47. Hattori N, et al. (2004) Preference of DNA methyltransferases for CpG islands in mouse embryonic stem cells. Genome Res 14(9):1733-1740.