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Volumn 40, Issue 2, 2013, Pages 983-997

A synthetic cadmium metallothionein gene (PMCd1syn) of Paramecium species: Expression, purification and characteristics of metallothionein protein

Author keywords

Cadmium; Ciliate protozoan; Metallothionein; PMCd1 gene; PMCd1syn gene; Synthetic gene

Indexed keywords

CADMIUM; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; METALLOTHIONEIN;

EID: 84878349184     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-012-2140-8     Document Type: Article
Times cited : (14)

References (53)
  • 1
    • 0023087396 scopus 로고
    • Chemistry and biochemistry of metallothionein
    • 2959513
    • Kägi JH, Kojima Y (1987) Chemistry and biochemistry of metallothionein. Experientia Suppl 52:25-61
    • (1987) Experientia Suppl , vol.52 , pp. 25-61
    • Kägi, J.H.1    Kojima, Y.2
  • 2
    • 0026353521 scopus 로고
    • Overview of metallothionein
    • 1779825 10.1016/0076-6879(91)05145-L 1:STN:280:DyaK387ktVWgtA%3D%3D
    • Kagi JH (1991) Overview of metallothionein. Meth Enzymol 205:613-626
    • (1991) Meth Enzymol , vol.205 , pp. 613-626
    • Kagi, J.H.1
  • 3
    • 0036240239 scopus 로고    scopus 로고
    • Metallothionein: The multipurpose protein
    • 10.1007/s00018-002-8454-2 1:CAS:528:DC%2BD38XjtlWmtbs%3D
    • Coyle P, Philcox JC, Carey LC, Rofe AM (2002) Metallothionein: the multipurpose protein. Cell Mol Life 59:627-647
    • (2002) Cell Mol Life , vol.59 , pp. 627-647
    • Coyle, P.1    Philcox, J.C.2    Carey, L.C.3    Rofe, A.M.4
  • 4
    • 0343090367 scopus 로고    scopus 로고
    • Metal thiolate clusters in neuronal growth inhibitor factor (GIF)
    • 10830840 10.1016/S0162-0134(99)00164-6 1:CAS:528:DC%2BD3cXjslyku7g%3D
    • Vasak M, Hasler DW, Faller P (2000) Metal thiolate clusters in neuronal growth inhibitor factor (GIF). J Inorg Biochem 79:7-10
    • (2000) J Inorg Biochem , vol.79 , pp. 7-10
    • Vasak, M.1    Hasler, D.W.2    Faller, P.3
  • 5
    • 80755132177 scopus 로고    scopus 로고
    • Metallothionein protein evolution: An miniassay
    • 21633816 10.1007/s00775-011-0798-3 1:CAS:528:DC%2BC3MXmvVKgt74%3D
    • Capdevila M, Atrian S (2011) Metallothionein protein evolution: an miniassay. J Biol Inorg Chem 16:977-989
    • (2011) J Biol Inorg Chem , vol.16 , pp. 977-989
    • Capdevila, M.1    Atrian, S.2
  • 6
    • 1542364374 scopus 로고    scopus 로고
    • MTs: Zinc, cadmium, mercury, and copper thiolates and selenolates mimicking protein active site features-structural aspects and biological implications
    • 14871142 10.1021/cr020620d 1:CAS:528:DC%2BD2cXosFOnsQ%3D%3D
    • Henkel G, Krebs B (2004) MTs: zinc, cadmium, mercury, and copper thiolates and selenolates mimicking protein active site features-structural aspects and biological implications. Chem Rev 104:801-824
    • (2004) Chem Rev , vol.104 , pp. 801-824
    • Henkel, G.1    Krebs, B.2
  • 7
    • 0036189730 scopus 로고    scopus 로고
    • Advances in structure and chemistry of metallothioneins
    • 10.1016/S0162-0134(01)00347-6
    • Isart NR, Vasak M (2002) Advances in structure and chemistry of metallothioneins. Inorg Biochem 88:388-396
    • (2002) Inorg Biochem , vol.88 , pp. 388-396
    • Isart, N.R.1    Vasak, M.2
  • 8
    • 0023937834 scopus 로고
    • Three-dimensional structure of rabbit liver [Cd7] metallothionein-2a in aqueous solution determined by nuclear magnetic resonance
    • 3418714 10.1016/0022-2836(88)90644-4 1:CAS:528:DyaL1cXksFSntrc%3D
    • Arseniev A, Schultze P, Wörgötter E, Braun W, Wagner G, Vasák M, Kägi JH, Wüthrich K (1988) Three-dimensional structure of rabbit liver [Cd7] metallothionein-2a in aqueous solution determined by nuclear magnetic resonance. J Mol Biol 201:637-657
    • (1988) J Mol Biol , vol.201 , pp. 637-657
    • Arseniev, A.1    Schultze, P.2    Wörgötter, E.3    Braun, W.4    Wagner, G.5    Vasák, M.6    Kägi, J.H.7    Wüthrich, K.8
  • 9
    • 0026454919 scopus 로고
    • Polypeptide fold into metal clusters of metallothionein 2 by nuclear magnetic resonance in solution
    • 1438200 10.1073/pnas.89.21.10124 1:CAS:528:DyaK3sXps1Gjsw%3D%3D
    • Braun W, Vasak M, Robbins AH, Stout CD, Wagner G, Kagi JHR, Wuthrich K (1992) Polypeptide fold into metal clusters of metallothionein 2 by nuclear magnetic resonance in solution. Proc natl Acad Sci USA 89:10124-10128
    • (1992) Proc Natl Acad Sci USA , vol.89 , pp. 10124-10128
    • Braun, W.1    Vasak, M.2    Robbins, A.H.3    Stout, C.D.4    Wagner, G.5    Kagi, J.H.R.6    Wuthrich, K.7
  • 10
    • 0030052073 scopus 로고    scopus 로고
    • 3D solution structure of copper and silver-substituted yeast metallothioneins
    • 10.1016/0014-5793(95)01492-6 1:CAS:528:DyaK28Xns12ntQ%3D%3D
    • Peterson CW, Narula SS, Armitage IM (1996) 3D solution structure of copper and silver-substituted yeast metallothioneins. Fed Europ Biochem Soc 379:85-93
    • (1996) Fed Europ Biochem Soc , vol.379 , pp. 85-93
    • Peterson, C.W.1    Narula, S.S.2    Armitage, I.M.3
  • 12
    • 0342614962 scopus 로고    scopus 로고
    • Three-dimensional solution structure of mouse [Cd7]-metallothionein-1 by homonuclear and heteronuclear NMR spectroscopy
    • 10631978 10.1110/ps.8.12.2630 1:CAS:528:DC%2BD3cXhtFynug%3D%3D
    • Zangger K, Oz G, Otvos JD, Armitage IM (1999) Three-dimensional solution structure of mouse [Cd7]-metallothionein-1 by homonuclear and heteronuclear NMR spectroscopy. Protein Sci 8:2630-2638
    • (1999) Protein Sci , vol.8 , pp. 2630-2638
    • Zangger, K.1    Oz, G.2    Otvos, J.D.3    Armitage, I.M.4
  • 13
    • 12344283772 scopus 로고    scopus 로고
    • How to hide zinc in a small protein
    • 15654738 10.1021/ar030182c 1:CAS:528:DC%2BD2cXpslentrk%3D
    • Blindauer CA, Sadler PJ (2005) How to hide zinc in a small protein. Acc Chem Res 38:62-69
    • (2005) Acc Chem Res , vol.38 , pp. 62-69
    • Blindauer, C.A.1    Sadler, P.J.2
  • 14
    • 0033955655 scopus 로고    scopus 로고
    • High resolution solution structure of the protein part of Cu7 metallothionein
    • 10672009 10.1046/j.1432-1327.2000.01093.x 1:CAS:528:DC%2BD3cXhsFGjtrs%3D
    • Bertini I, Hartmann HJ, Klein T, Liu GH, Luchinat C, Weser U (2000) High resolution solution structure of the protein part of Cu7 metallothionein. Eur J Biochem 267:1008-1018
    • (2000) Eur J Biochem , vol.267 , pp. 1008-1018
    • Bertini, I.1    Hartmann, H.J.2    Klein, T.3    Liu, G.H.4    Luchinat, C.5    Weser, U.6
  • 15
    • 0035949634 scopus 로고    scopus 로고
    • Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: Metallothionein-3
    • 11560491 10.1021/bi010827l 1:STN:280:DC%2BD3Mrht1ymtQ%3D%3D
    • Oz G, Zangger K, Armitage IM (2001) Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3. Biochemistry 40:11433-11441
    • (2001) Biochemistry , vol.40 , pp. 11433-11441
    • Oz, G.1    Zangger, K.2    Armitage, I.M.3
  • 16
    • 0036937216 scopus 로고    scopus 로고
    • Structure of the Cd-113(3) beta domains from Homarus americanus metallothionein-1: Hydrogen bonding and solvent accessibility of sulfur atoms
    • 12203008 10.1007/s00775-002-0345-3 1:CAS:528:DC%2BD38XotlajsLY%3D
    • Munoz A, Forsterling FH, Shaw CF, Petering DH (2002) Structure of the Cd-113(3) beta domains from Homarus americanus metallothionein-1: hydrogen bonding and solvent accessibility of sulfur atoms. J Biol Inorg Chem 7:713-724
    • (2002) J Biol Inorg Chem , vol.7 , pp. 713-724
    • Munoz, A.1    Forsterling, F.H.2    Shaw, C.F.3    Petering, D.H.4
  • 17
    • 0037387135 scopus 로고    scopus 로고
    • Solution structure of MT-nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps
    • 12679021 10.1016/S0969-2126(03)00044-3 1:CAS:528:DC%2BD3sXislelu74%3D
    • Capasso C, Carginale V, Crescenzi O, Di Maro D, Parisi E, Spadaccini R, Temussi PA (2003) Solution structure of MT-nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps. Structure 11:435-443
    • (2003) Structure , vol.11 , pp. 435-443
    • Capasso, C.1    Carginale, V.2    Crescenzi, O.3    Di Maro, D.4    Parisi, E.5    Spadaccini, R.6    Temussi, P.A.7
  • 18
    • 8644225574 scopus 로고    scopus 로고
    • Solution structure of Cu6 metallothionein from the fungus Neurospora
    • 15511227 10.1111/j.1432-1033.2004.04361.x 1:CAS:528:DC%2BD2cXhtVWhurbE
    • Cobine PA, McKay RT, Zangger K, Dameron CT, Armitage IM (2004) Solution structure of Cu6 metallothionein from the fungus Neurospora. Eur J Biochem 271:4213-4221
    • (2004) Eur J Biochem , vol.271 , pp. 4213-4221
    • Cobine, P.A.1    McKay, R.T.2    Zangger, K.3    Dameron, C.T.4    Armitage, I.M.5
  • 20
    • 31444449685 scopus 로고    scopus 로고
    • The molecular structure and vibrational spectra of corrolazine metal complexes, CzM by density functional theory
    • 10.1016/j.febslet.2005.12.099 1:CAS:528:DC%2BD28XhtFejsLc%3D
    • Wang H, Zhang Q, Cai B, Li HY, Sze KH, Huang ZX, Wu HM, Sun HZ (2006) The molecular structure and vibrational spectra of corrolazine metal complexes, CzM by density functional theory. FEBS J 580:795-800
    • (2006) FEBS J , vol.580 , pp. 795-800
    • Wang, H.1    Zhang, Q.2    Cai, B.3    Li, H.Y.4    Sze, K.H.5    Huang, Z.X.6    Wu, H.M.7    Sun, H.Z.8
  • 21
    • 61349154809 scopus 로고    scopus 로고
    • The beta(E)-domain of wheat E(c)1 metallothionein: A metal-binding domain with a distinctive structure
    • 19361445 10.1016/j.jmb.2009.01.035 1:CAS:528:DC%2BD1MXivVegtb0%3D
    • Peroza EA, Schmucki R, Guntert P, Freisinger E, Zerbe O (2009) The beta(E)-domain of wheat E(c)1 metallothionein: a metal-binding domain with a distinctive structure. J Mol Biol 387:207-218
    • (2009) J Mol Biol , vol.387 , pp. 207-218
    • Peroza, E.A.1    Schmucki, R.2    Guntert, P.3    Freisinger, E.4    Zerbe, O.5
  • 23
    • 84865988176 scopus 로고    scopus 로고
    • Isolation of metallothionein genes and in silico structural characterization of their proteins using molecular modeling from Yak (Bos grunniens)
    • 10.1007/s10528-012-9503-7
    • Zhang L, Wu J, Wang X, Liu B, Ma B (2012) Isolation of metallothionein genes and in silico structural characterization of their proteins using molecular modeling from Yak (Bos grunniens). Biochem Genet. doi: 10.1007/s10528-012-9503-7
    • (2012) Biochem Genet
    • Zhang, L.1    Wu, J.2    Wang, X.3    Liu, B.4    Ma, B.5
  • 24
    • 0034990167 scopus 로고    scopus 로고
    • Microbial metallothioneins
    • 11407113 10.1016/S0065-2911(01)44014-8 1:CAS:528:DC%2BD3MXltV2msr4%3D
    • Robinson NJ, Whitehall SK, Cavet JS (2001) Microbial metallothioneins. Adv Microb Physiol 44:183-213
    • (2001) Adv Microb Physiol , vol.44 , pp. 183-213
    • Robinson, N.J.1    Whitehall, S.K.2    Cavet, J.S.3
  • 25
    • 78349307650 scopus 로고    scopus 로고
    • Role of MT in cadmium traffic and toxicity in kidneys and other mammalian organs
    • 20549307 10.1007/s10534-010-9351-z
    • Sabolić I, Breljak D, Skarica M, Herak-Kramberger CM (2010) Role of MT in cadmium traffic and toxicity in kidneys and other mammalian organs. Biometals 23:897-926
    • (2010) Biometals , vol.23 , pp. 897-926
    • Sabolić, I.1    Breljak, D.2    Skarica, M.3    Herak-Kramberger, C.M.4
  • 27
    • 33644780954 scopus 로고    scopus 로고
    • A family knockout of all four Drosophila MTs reveals a central role in copper homeostasis and detoxification
    • 16508004 10.1128/MCB.26.6.2286-2296.2006 1:CAS:528:DC%2BD28Xislygtbs%3D
    • Egli D, Yepiskoposyan H, Selvaraj A, Balamurugan K, Rajaram R (2006) A family knockout of all four Drosophila MTs reveals a central role in copper homeostasis and detoxification. Mol Cell Biol 26:2286-2296
    • (2006) Mol Cell Biol , vol.26 , pp. 2286-2296
    • Egli, D.1    Yepiskoposyan, H.2    Selvaraj, A.3    Balamurugan, K.4    Rajaram, R.5
  • 28
    • 33751409084 scopus 로고    scopus 로고
    • Single and double MT knockout in the nematode C. elegans reveals cadmium dependent and independent toxic effects on life history traits
    • 17141712 10.1016/j.envpol.2006.06.003 1:CAS:528:DC%2BD28Xht1KgtbnL
    • Hughes S, Sturzenbaum SR (2007) Single and double MT knockout in the nematode C. elegans reveals cadmium dependent and independent toxic effects on life history traits. Environ Pollut 145:395-400
    • (2007) Environ Pollut , vol.145 , pp. 395-400
    • Hughes, S.1    Sturzenbaum, S.R.2
  • 31
    • 79955068293 scopus 로고    scopus 로고
    • Functional comparison of metallothionein MTT1 and MTT2 from Tetrahymena thermophile
    • 21352798 10.1016/j.abb.2011.02.015 1:CAS:528:DC%2BC3MXltFyhtbw%3D
    • Wang Q, Xu J, Chai B, Liang A, Wang W (2011) Functional comparison of metallothionein MTT1 and MTT2 from Tetrahymena thermophile. Arch Biochem Biophys 509:170-176
    • (2011) Arch Biochem Biophys , vol.509 , pp. 170-176
    • Wang, Q.1    Xu, J.2    Chai, B.3    Liang, A.4    Wang, W.5
  • 32
    • 33646573348 scopus 로고    scopus 로고
    • Cloning and characterization of a new multi-stress inducible metallothionein gene in Tetrahymena pyriformis
    • 16621695 10.1016/j.protis.2006.02.006 1:CAS:528:DC%2BD28XpsFGrtLo%3D
    • Fu C, Miao W (2006) Cloning and characterization of a new multi-stress inducible metallothionein gene in Tetrahymena pyriformis. Protist 157:193-203
    • (2006) Protist , vol.157 , pp. 193-203
    • Fu, C.1    Miao, W.2
  • 33
    • 0032967071 scopus 로고    scopus 로고
    • Cadmium metallothionein gene of Tetrahymena pyriformis
    • 10393238 10.1016/S0378-1119(99)00181-X 1:CAS:528:DyaK1MXks1CqsL8%3D
    • Piccinni E, Bertaggia D, Santovito G, Miceli C, Kraen A (1999) Cadmium metallothionein gene of Tetrahymena pyriformis. Gene 234:51-59
    • (1999) Gene , vol.234 , pp. 51-59
    • Piccinni, E.1    Bertaggia, D.2    Santovito, G.3    Miceli, C.4    Kraen, A.5
  • 34
    • 3042728574 scopus 로고    scopus 로고
    • Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila
    • 15305793 10.1078/143446104774199565 1:CAS:528:DC%2BD2cXnsVCms7w%3D
    • Dondero F, Cavaletto M, Chezzi AR, La Terza A, Banni M, Viarengo A (2004) Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila. Protist 155:157-168
    • (2004) Protist , vol.155 , pp. 157-168
    • Dondero, F.1    Cavaletto, M.2    Chezzi, A.R.3    La Terza, A.4    Banni, M.5    Viarengo, A.6
  • 35
    • 0037133578 scopus 로고    scopus 로고
    • A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila
    • 11891286 10.1073/pnas.052016199 1:CAS:528:DC%2BD38Xis1Kltrk%3D
    • Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proc Natl Acad Sci USA 99:3734-3739
    • (2002) Proc Natl Acad Sci USA , vol.99 , pp. 3734-3739
    • Shang, Y.1    Song, X.2    Bowen, J.3    Corstanje, R.4    Gao, Y.5    Gaertig, J.6    Gorovsky, M.A.7
  • 36
    • 35548995934 scopus 로고    scopus 로고
    • Identification, cloning and sequencing of a novel stress inducible metallothionein gene from locally isolated Tetrahymena tropicalis lahorensis
    • 10.1016/j.gene.2007.08.019
    • Shuja RN, Shakoori AR (2007) Identification, cloning and sequencing of a novel stress inducible metallothionein gene from locally isolated Tetrahymena tropicalis lahorensis. Gene 4905:19-26
    • (2007) Gene , vol.4905 , pp. 19-26
    • Shuja, R.N.1    Shakoori, A.R.2
  • 37
    • 50849101019 scopus 로고    scopus 로고
    • Two new members of the Tetrahymena multi-stress inducible metallothionein family: Characterization and expression analysis of T. rostrata Cd/Cu metallothionein genes
    • 18675326 10.1016/j.gene.2008.07.002 1:CAS:528:DC%2BD1cXhtVOqsrfF
    • Amaro F, de Lucas MDP, Martin-Gonzalez A, Gutierrez JC (2008) Two new members of the Tetrahymena multi-stress inducible metallothionein family: characterization and expression analysis of T. rostrata Cd/Cu metallothionein genes. Gene 423:85-91
    • (2008) Gene , vol.423 , pp. 85-91
    • Amaro, F.1    De Lucas, M.D.P.2    Martin-Gonzalez, A.3    Gutierrez, J.C.4
  • 39
    • 0036746478 scopus 로고    scopus 로고
    • Metal interaction and regulation of Tertrahymena pigmentosa metallothionein genes
    • 12389817 10.1078/1434-4610-00105 1:CAS:528:DC%2BD38XovVGrtbw%3D
    • Boldrin F, Santovito G, Irato P, Piccini E (2002) Metal interaction and regulation of Tertrahymena pigmentosa metallothionein genes. Protist 153:283-291
    • (2002) Protist , vol.153 , pp. 283-291
    • Boldrin, F.1    Santovito, G.2    Irato, P.3    Piccini, E.4
  • 40
    • 77956266030 scopus 로고    scopus 로고
    • A circadian clock regulates sensitivity to cadmium in Paramecium tetraaurelia
    • 20108033 10.1007/s10565-010-9150-x 1:CAS:528:DC%2BC3cXotlyku7g%3D
    • Hinrichsen RD, Tran JR (2010) A circadian clock regulates sensitivity to cadmium in Paramecium tetraaurelia. Cell Biol Toxicol 26:379-389
    • (2010) Cell Biol Toxicol , vol.26 , pp. 379-389
    • Hinrichsen, R.D.1    Tran, J.R.2
  • 41
    • 60549107238 scopus 로고    scopus 로고
    • Identification and cloning of first cadmium metallothionein like gene from locally isolated ciliate, Paramecium sp
    • 18273691 10.1007/s11033-008-9213-8 1:CAS:528:DC%2BD1MXhvF2ltL0%3D
    • Shuja RN, Shakoori AR (2009) Identification and cloning of first cadmium metallothionein like gene from locally isolated ciliate, Paramecium sp. Mol Biol Rep 36:549-560
    • (2009) Mol Biol Rep , vol.36 , pp. 549-560
    • Shuja, R.N.1    Shakoori, A.R.2
  • 43
    • 0019162146 scopus 로고
    • Cloning in single stranded bacteriophage as an aid to rapid DNA sequencing
    • 6260957 10.1016/0022-2836(80)90196-5 1:CAS:528:DyaL3MXpvFWqtQ%3D%3D
    • Sanger F, Coulson AR, Barrell GG, Smith AJH, Roe BA (1980) Cloning in single stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143:161-178
    • (1980) J Mol Biol , vol.143 , pp. 161-178
    • Sanger, F.1    Coulson, A.R.2    Barrell, G.G.3    Smith, A.J.H.4    Roe, B.A.5
  • 44
    • 0017681196 scopus 로고
    • DNA sequencing with chain-terminating inhibitors
    • 271968 10.1073/pnas.74.12.5463 1:CAS:528:DyaE1cXhtlaru7Y%3D
    • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc natl Acad Sci USA 74:5463-5467
    • (1977) Proc Natl Acad Sci USA , vol.74 , pp. 5463-5467
    • Sanger, F.1    Nicklen, S.2    Coulson, A.R.3
  • 45
    • 78649975294 scopus 로고    scopus 로고
    • Cd(II) and AS(III) bioaccumulation by recombinant Escherichia coli expressing oligomeric human metallothioneins
    • 10.1016/j.jhazmat.2010.10.051 1:CAS:528:DC%2BC3cXhsFCks7nL
    • Ma Y, Lin J, Zhang C, Ren Y, Lin J (2011) Cd(II) and AS(III) bioaccumulation by recombinant Escherichia coli expressing oligomeric human metallothioneins. J Haz Mat 185:1605-1608
    • (2011) J Haz Mat , vol.185 , pp. 1605-1608
    • Ma, Y.1    Lin, J.2    Zhang, C.3    Ren, Y.4    Lin, J.5
  • 46
    • 0034466827 scopus 로고    scopus 로고
    • Cytoplasmic expression of a soluble synthetic mammalian metallothionein-α domain in Escherichia coli: Enhanced tolerance and accumulation of cadmium
    • 10.1385/MB:16:3:211
    • Li Y, Cockburn W, Kilpatrick J, Whitelam GC (2000) Cytoplasmic expression of a soluble synthetic mammalian metallothionein-α domain in Escherichia coli: enhanced tolerance and accumulation of cadmium. Mol. Biotech 16:211-219
    • (2000) Mol. Biotech , vol.16 , pp. 211-219
    • Li, Y.1    Cockburn, W.2    Kilpatrick, J.3    Whitelam, G.C.4
  • 47
    • 0023840783 scopus 로고
    • Efficient expression of the yeast metallothionein gene in Escherichia coli
    • Berka T, Shatzman A, Zimmerman J, Strickler JM, Rosenberg M (1988) Efficient expression of the yeast metallothionein gene in Escherichia coli. J Bact 170:210-226
    • (1988) J Bact , vol.170 , pp. 210-226
    • Berka, T.1    Shatzman, A.2    Zimmerman, J.3    Strickler, J.M.4    Rosenberg, M.5
  • 48
    • 0027467849 scopus 로고
    • Cloning and expression of Saccharomyces cerevisiae copper metallothionein gene in Escherichia coli and characterization of recombinant protein
    • 8444189 10.1111/j.1432-1033.1993.tb17689.x 1:CAS:528:DyaK3sXhvVKhtrs%3D
    • Sayers Z, Brouillon P, Vorgias CE, Nolting HF, Hermes C, Koch MH (1993) Cloning and expression of Saccharomyces cerevisiae copper metallothionein gene in Escherichia coli and characterization of recombinant protein. Eur J Biochem 212:521-528
    • (1993) Eur J Biochem , vol.212 , pp. 521-528
    • Sayers, Z.1    Brouillon, P.2    Vorgias, C.E.3    Nolting, H.F.4    Hermes, C.5    Koch, M.H.6
  • 49
    • 0023133003 scopus 로고
    • Expression of cloned monkey metallothionein in Escherichia coli
    • 3103531 1:CAS:528:DyaL2sXhtlaqtLo%3D
    • Murooka Y, Nagaoka T (1987) Expression of cloned monkey metallothionein in Escherichia coli. Appl Environ Microbiol 53:204-207
    • (1987) Appl Environ Microbiol , vol.53 , pp. 204-207
    • Murooka, Y.1    Nagaoka, T.2
  • 50
    • 0023691990 scopus 로고
    • Expression of the mouse metallothionein -1 gene in Escherichia coli: Increased tolerance to heavy metals
    • 3056525 10.1016/0167-4781(88)90045-0 1:CAS:528:DyaL1MXjsVOmtg%3D%3D
    • Hou YM, Kim R, Kim SH (1988) Expression of the mouse metallothionein -1 gene in Escherichia coli: increased tolerance to heavy metals. Biochim Biophys Acta 951:230-234
    • (1988) Biochim Biophys Acta , vol.951 , pp. 230-234
    • Hou, Y.M.1    Kim, R.2    Kim, S.H.3
  • 51
    • 0025278204 scopus 로고
    • The expression of a synthetic rainbow trout metallothionein gene in E. coli
    • 2182123 10.1016/0167-4781(90)90054-6 1:CAS:528:DyaK3cXktFyrtLY%3D
    • Kille P, Stephens P, Cryer A, KLay J (1990) The expression of a synthetic rainbow trout metallothionein gene in E. coli. Biochim Biophys Acta 1048:178-186
    • (1990) Biochim Biophys Acta , vol.1048 , pp. 178-186
    • Kille, P.1    Stephens, P.2    Cryer, A.3    Klay, J.4
  • 53
    • 80755146147 scopus 로고    scopus 로고
    • Ciliate metallothioneins: Unique microbial eukaryotic heavy-metal binder molecules
    • 21785894 10.1007/s00775-011-0820-9 1:CAS:528:DC%2BC3MXptFymurg%3D
    • Gutierrez JC, Amaro F, Diaz S, de Francisco P, Cubas LL, Martin-Gonzalez A (2011) Ciliate metallothioneins: unique microbial eukaryotic heavy-metal binder molecules. J Biol Inorg Chem 16:1025-1034
    • (2011) J Biol Inorg Chem , vol.16 , pp. 1025-1034
    • Gutierrez, J.C.1    Amaro, F.2    Diaz, S.3    De Francisco, P.4    Cubas, L.L.5    Martin-Gonzalez, A.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.