Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9

Journal of Biological Chemistry

Volumn 288, Issue 21, 2013, Pages 15142-15147

  Hu, Qi ^a   Wu, Di ^a   Chen, Wen ^b   Yan, Zhen ^a   Shi, Yigong ^a  

^a TSINGHUA UNIVERSITY   (China)

^b PEKING UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSOMES; EXPERIMENTAL DATUM; HOMODIMERS; ORDERS OF MAGNITUDE; PROTEASE ACTIVITIES; PROTEOLYTIC ACTIVITIES; PROTEOLYTIC PROCESSING; TRIPLE MUTANTS;

CELL DEATH;

ENZYME ACTIVITY;

APOPTOSOME; CASPASE 9; ENZYME PRECURSOR; PROTEINASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME DEGRADATION; ESCHERICHIA COLI; GEL FILTRATION; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; WILD TYPE;

APAF-1; APOPTOSIS; CASPASE; CELL BIOLOGY; CELL DEATH; ENZYME MECHANISMS;

AMINO ACID SUBSTITUTION; APOPTOSOMES; APOPTOTIC PROTEASE-ACTIVATING FACTOR 1; CASPASE 9; ENZYME ACTIVATION; ENZYME PRECURSORS; HUMANS; MUTATION, MISSENSE; PROTEIN MULTIMERIZATION; PROTEOLYSIS;

EID: 84878242246     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.441568     Document Type: Article

References (23)

1. Danial, N. N., and Korsmeyer, S. J. (2004) Cell death: critical control points. Cell 116, 205-219 (Pubitemid 38167313)
2. Fuchs, Y., and Steller, H. (2011) Programmed cell death in animal development and disease. Cell 147, 742-758
3. Lavrik, I., Golks, A., and Krammer, P. H. (2005) Death receptor signaling. J. Cell Sci. 118, 265-267 (Pubitemid 40277339)
4. Riedl, S. J., and Shi, Y. (2004) Molecular mechanisms of caspase regulation during apoptosis. Nat. Rev. Mol. Cell Biol. 5, 897-907 (Pubitemid 39486541)
5. Shi, Y. (2002) Mechanisms of caspase activation and inhibition during apoptosis. Mol. Cell 9, 459-470
6. Mace, P. D., and Riedl, S. J. (2010) Molecular cell death platforms and assemblies. Curr. Opin. Cell Biol. 22, 828-836
7. Liu, X., Kim, C. N., Yang, J., Jemmerson, R., and Wang, X. (1996) Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86, 147-157 (Pubitemid 26256586)
8. Zou, H., Henzel, W. J., Liu, X., Lutschg, A., and Wang, X. (1997) Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90, 405-413 (Pubitemid 27347231)
9. Acehan, D., Jiang, X., Morgan, D. G., Heuser, J. E., Wang, X., and Akey, C. W. (2002) Three-dimensional structure of the apoptosome: implications for assembly, procaspase-9 binding, and activation. Mol. Cell 9, 423-432 (Pubitemid 34195566)
10. Rodriguez, J., and Lazebnik, Y. (1999) Caspase-9 and APAF-1 form an active holoenzyme. Genes Dev. 13, 3179-3184 (Pubitemid 30030439)
11. Malladi, S., Challa-Malladi, M., Fearnhead, H. O., and Bratton, S. B. (2009) The Apaf-1·procaspase-9 apoptosome complex functions as a proteolytic-based molecular timer. EMBO J. 28, 1916-1925
12. Renatus, M., Stennicke, H. R., Scott, F. L., Liddington, R. C., and Salvesen, G. S. (2001) Dimer formation drives the activation of the cell death protease caspase-9. Proc. Natl. Acad. Sci. U.S.A. 98, 14250-14255 (Pubitemid 33121378)
13. Srinivasula, S. M., Hegde, R., Saleh, A., Datta, P., Shiozaki, E., Chai, J., Lee, R. A., Robbins, P. D., Fernandes-Alnemri, T., Shi, Y., and Alnemri, E. S. (2001) A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosis. Nature 410, 112-116 (Pubitemid 32225846)
14. Stennicke, H. R., Deveraux, Q. L., Humke, E. W., Reed, J. C., Dixit, V. M., and Salvesen, G. S. (1999) Caspase-9 can be activated without proteolytic processing. J. Biol. Chem. 274, 8359-8362 (Pubitemid 29164620)
15. Riedl, S. J., Li, W., Chao, Y., Schwarzenbacher, R., and Shi, Y. (2005) Structure of the apoptotic protease-activating factor 1 bound to ADP. Nature 434, 926-933 (Pubitemid 40559008)
16. Srinivasula, S. M., Ahmad, M., Fernandes-Alnemri, T., and Alnemri, E. S. (1998) Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization. Mol. Cell 1, 949-957 (Pubitemid 128379256)
17. Boatright, K. M., Renatus, M., Scott, F. L., Sperandio, S., Shin, H., Pedersen, I. M., Ricci, J. E., Edris, W. A., Sutherlin, D. P., Green, D. R., and Salvesen, G. S. (2003) A unified model for apical caspase activation. Mol. Cell 11, 529-541 (Pubitemid 36293843)
18. Donepudi, M., Mac Sweeney, A., Briand, C., and Grütter, M. G. (2003) Insights into the regulatory mechanism for caspase-8 activation. Mol. Cell 11, 543-549 (Pubitemid 36297058)
19. Huang, Y., Rich, R. L., Myszka, D. G., and Wu, H. (2003) Requirement of both the second and third BIR domains for the relief of X-linked inhibitor of apoptosis protein (XIAP)-mediated caspase inhibition by Smac. J. Biol. Chem. 278, 49517-49522
20. Zou, H., Yang, R., Hao, J., Wang, J., Sun, C., Fesik, S. W., Wu, J. C., Tomaselli, K. J., and Armstrong, R. C. (2003) Regulation of the Apaf-1/caspase-9 apoptosome by caspase-3 and XIAP. J. Biol. Chem. 278, 8091-8098 (Pubitemid 36800549)
21. Gao, Z., Tian, Y., Wang, J., Yin, Q., Wu, H., Li, Y. M., and Jiang, X. (2007) A dimeric Smac/Diablo peptide directly relieves caspase-3 inhibition by XIAP. Dynamic and cooperative regulation of XIAP by Smac/Diablo. J. Biol. Chem. 282, 30718-30727 (Pubitemid 350035215)
22. Burke, S. P., Smith, L., and Smith, J. B. (2010) cIAP1 cooperatively inhibits procaspase-3 activation by the caspase-9 apoptosome. J. Biol. Chem. 285, 30061-30068
23. Keller, N., Mareš, J., Zerbe, O., and Grütter, M. G. (2009) Structural and biochemical studies on procaspase-8: new insights on initiator caspase activation. Structure 17, 438-448