Influence of copy number on the expression levels of pandemic influenza hemagglutinin recombinant protein in methylotrophic yeast Pichia pastoris

Virus Genes

Volumn 45, Issue 3, 2012, Pages 440-451

  Athmaram, T N ^a   Saraswat, Shweta ^a   Singh, Anil Kumar ^a   Rao, M Kameswara ^a   Gopalan, N ^a   Suryanarayana, V V S ^b   Rao, P V L ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

^b INDIAN VETERINARY RESEARCH INSTITUTE   (India)

Author keywords

Copy number, swine origin influenza A virus; Gene dosage effect; H1N1; Hemagglutinin; Pichia pastoris; Yeast

Indexed keywords

ANTIBIOTIC G 418; INFLUENZA VIRUS HEMAGGLUTININ; RECOMBINANT HEMAGGLUTININ; RECOMBINANT PROTEIN; SYNTHETIC DNA; UNCLASSIFIED DRUG; H1N1 VIRUS HEMAGGLUTININ;

ARTICLE; CELL CLONE; CONTROLLED STUDY; DNA INTEGRATION; GENE CASSETTE; GENE DOSAGE; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; HEMAGGLUTININ GENE; HETEROLOGOUS EXPRESSION; HOMOLOGOUS RECOMBINATION; INFLUENZA VIRUS A H1N1; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SUPERNATANT; TRANSGENE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; MASS SPECTROMETRY; METABOLISM; MICROBIAL SENSITIVITY TEST; MOLECULAR CLONING; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; TRANSGENIC ORGANISM; VIRUS GENE;

INFLUENZA A VIRUS; PICHIA; PICHIA PASTORIS; SUIDAE;

CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; GENES, VIRAL; GENETIC VECTORS; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HOMOLOGOUS RECOMBINATION; INFLUENZA A VIRUS, H1N1 SUBTYPE; MICROBIAL SENSITIVITY TESTS; ORGANISMS, GENETICALLY MODIFIED; PICHIA; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSFORMATION, GENETIC; TRANSGENES;

EID: 84878235326     PISSN: 09208569     EISSN: 1572994X     Source Type: Journal    
DOI: 10.1007/s11262-012-0809-7     Document Type: Article

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