Isotopically labeled expression in E. coli, purification, and refolding of the full ectodomain of the influenza virus membrane fusion protein

Protein Expression and Purification

Volumn 61, Issue 2, 2008, Pages 212-219

  Curtis Fisk, Jaime ^a   Spencer, Ryan M ^a   Weliky, David P ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Bacterial expression; Circular dichroism; Hemagglutinin; Influenza; Isotopic labeling; Membrane proteins; NMR; Protein expression; Protein refolding; Solid state NMR; Viral proteins

Indexed keywords

CARBON; GLUCOSE; GLYCEROL; VIRUS FUSION PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL PROLIFERATION; CHEMISTRY; CIRCULAR DICHROISM; DOSE RESPONSE; DRUG EFFECT; ESCHERICHIA COLI; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; ISOTOPE LABELING; MEMBRANE FUSION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; SOLUBILITY; STANDARD; TIME;

AMINO ACID SEQUENCE; CARBON ISOTOPES; CELL PROLIFERATION; CELLS, CULTURED; CIRCULAR DICHROISM; CLONING, MOLECULAR; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GLUCOSE; GLYCEROL; HYDROGEN-ION CONCENTRATION; ISOTOPE LABELING; MAGNETIC RESONANCE SPECTROSCOPY; MEMBRANE FUSION; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; REFERENCE STANDARDS; SOLUBILITY; TIME FACTORS; VIRAL FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORTHOMYXOVIRIDAE;

EID: 50049096697     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.06.009     Document Type: Article

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