Comparison of five assays for detection of Clostridium difficile toxin

Journal of Molecular Diagnostics

Volumn 13, Issue 4, 2011, Pages 395-400

  Chapin, Kimberle C ^a,b   Dickenson, Roberta A ^a   Wu, Fongman ^a   Andrea, Sarah B ^a  

^a RHODE ISLAND HOSPITAL   (United States)

^b BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B; GLUTAMATE DEHYDROGENASE; BACTERIAL PROTEIN; BACTERIAL TOXIN; ENTEROTOXIN; TCDA PROTEIN, CLOSTRIDIUM DIFFICILE; TOXB PROTEIN, CLOSTRIDIUM DIFFICILE;

ADULT; ARTICLE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; COST EFFECTIVENESS ANALYSIS; ENZYME IMMUNOASSAY; FECES ANALYSIS; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NONHUMAN; SENSITIVITY AND SPECIFICITY; SYMPTOM; TOXIN ANALYSIS; ADOLESCENT; AGED; CHEMISTRY; CHILD; COMPARATIVE STUDY; COST BENEFIT ANALYSIS; ECONOMICS; FECES; FEMALE; MALE; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; MOLECULAR DIAGNOSIS; PRESCHOOL CHILD; PSEUDOMEMBRANOUS COLITIS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIAL PROTEINS; BACTERIAL TOXINS; CHILD; CHILD, PRESCHOOL; CLOSTRIDIUM DIFFICILE; COST-BENEFIT ANALYSIS; ENTEROCOLITIS, PSEUDOMEMBRANOUS; ENTEROTOXINS; FECES; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 80054075999     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmoldx.2011.03.004     Document Type: Article

References (27)

1. Tedesco F, Stanley R, Alpar D: Diagnostic features of clindamycin-associated pseudomembranous colitis. N Engl J Med 1974, 290:841-843
2. Bartlett J: Narrative review: the new epidemic of Clostridium difficile-associated enteric disease. Ann Intern Med 2006, 145:758-764
3. Fellmeth G, Yarlagadda S, Iyer S: Epidemiology of community-onset Clostridium difficile infection in a community in the South of England. J Infect Public Health 2010, 3:118-123
4. Durai R: Epidemiology, pathogenesis, and management of Clostridium difficile infection. Dig Dis Sci 2007, 52:2958-2962
5. Curry S: Clostridium difficile. Clin Lab Med 2010, 30:329-342
6. Yoo J, Lightner AL: Clostridium difficile infections: what every clinician should know. Permanente J 2010, 14:35-40
7. Salkind AR: Clostridium difficile: an update for the primary care clinician. South Med J 2010, 103:896-900
8. Bobo L, Dubberke E: Recognition and prevention of hospital-associated enteric infections in the intensive care unit. Crit Care Med 2010, 38:S324-S334
9. Eastwood K, Else P, Charlett A, Wilcox M: Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J Clin Microbiol 2009, 47:3211-3217
10. Kvach EJ, Ferguson D, Riska PF, Landry ML: Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection. J Clin Microbiol 2010, 48:109-114
11. Terhes G, Urban E, Soki J, Nacsa E, Nagy E: Comparison of a rapid molecular method, the BD GeneOhm Cdiff assay, to the most frequently used laboratory tests for detection of toxin-producing Clostridium difficile in diarrheal feces. J Clin Microbiol 2009, 47:3478-3481
12. Hawass N: Comparing the sensitivities and specificities of two diagnostic procedures performed on the same group of patients. Br J Radiol 1997, 70:360-366
13. American College of Physicians: Primer on cost-effectiveness analysis. Effective Clin Pract 2000, 5:253-255
14. Pisapia J: Cost Analysis and Learning Technologies: How to conduct a cost-effectiveness analysis. Richmond, VA: Virginia Commonwealth University, School of Education, Metropolitan Education Research Consortium; 1994
15. Larson A, Fung A, Fang F: Evaluation of tcdB real-time PCR in a three-step diagnostic algorithm for detection of toxigenic Clostridium difficile. J Clin Microbiol 2010, 48:124-130
16. Novak-Weekley S, Marlowe EM, Miller JM, Cumpio J, Nomura JH, Vance PH, Weissfeld A: Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms. J Clin Microbiol 2010, 48:889-893
17. Stamper P, Alcabasa R, Aird D, Babiker W, Wehrlin J, Ikpeama I, Carroll KC: Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples. J Clin Microbiol 2009, 47:373-378
18. Swindells J, Brenwald N, Reading N, Oppenheim B: Evaluation of diagnostic tests for Clostridium difficile infection. J Clin Microbiol 2010, 48:606-608
19. Sharp S, Ruden L, Pohl JC, Hatcher PA, Jayne LM, Ivie WM: Evaluation of the C. Diff Quik Chek Complete Assay, a new glutamate dehydrogenase and A/B toxin combination lateral flow assay for use in rapid, simple diagnosis of Clostridium difficile disease. J Clin Microbiol 2010, 48:2082-2086
20. Schmidt M, Gilligan P: Clostridium difficile testing algorithms: what is practical and feasible? . Anaerobe 2009, 15:270-273
21. Reller M, Alcabasa R, Lema C, Carroll KC: Comparison of two rapid assays for Clostridium difficile common antigen and aCdifficile toxin A/B assay with the cell culture neutralization assay. Am J Clin Pathol 2010, 133:107-109
22. Dubberke ER, Wertheimer A: Review of current literature on the economic burden of Clostridium difficile infection. Infect Control Hospital Epidemiol 2009, 30:57-66
23. Nguyen G, Kaplan G, Harris ML, Brant SR: A national survey of the prevalence and impact of Clostridium difficile infection among hospitalized inflammatory bowel disease patients. Am J Gastroenterol 2008, 103:1443-1450 (Pubitemid 351813903)
24. O'Brien JA, Lahue BJ, Caro JJ, Davidson DM: The emerging infectious challenge of Clostridium difficile-associated disease in Massachusetts hospitals: clinical and economic consequences. Infect Control Hosp Epidemiol 2007, 28:1219-1227 (Pubitemid 47596558)
25. Cherifi S, Delmee M, Van Broeck J, Beyer I, Byl B, Mascart G: Management of an outbreak of Clostridium difficile-associated disease among geriatric patients. Infect Control Hosp Epidemiol 2006, 27:1200-1205 (Pubitemid 44807463)
26. Poon EG, Gandhi TK, Sequist TD, Murff HJ, Karson HJ, Bates DW: "I wish I had seen this test result earlier!": dissatisfaction with test result management systems in primary care. Arch Intern Med 2004, 164:2223-2228 (Pubitemid 39452982)
27. Koster M, Daigneault L, Dickenson R, et al.: Utilization of Rapid PCR Testing for Enterovirus and Impact on Physician Practice. Boston, MA: 2008;C-238