CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

Journal of Applied Oral Science

Volumn 21, Issue 2, 2013, Pages 99-105

  Sipert, Carla Renata ^a   Morandini, Ana Carolina de Faria ^a   Modena, Karin Cristina da Silva ^a   Dionisio, Thiago Jose ^a   Machado, Maria Aparecida Andrade Moreira ^a   de Oliveira, Sandra Helena Penha ^b   Campanelli, Ana Paula ^a   Santos, Carlos Ferreira ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

CCL3 chemokine; Chemokines; CXCL12 chemokine; Dental pulp; Dental pulp inflammation; Fibroblasts

Indexed keywords

MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA; STROMAL CELL DERIVED FACTOR 1;

ANALYSIS OF VARIANCE; BIOSYNTHESIS; CELL CULTURE; CELL SURVIVAL; CYTOLOGY; DECIDUOUS TOOTH; DENTITION; ENZYME LINKED IMMUNOSORBENT ASSAY; FIBROBLAST; HUMAN; IN VITRO STUDY; METABOLISM; PORPHYROMONAS GINGIVALIS; TIME; TOOTH PULP;

ANALYSIS OF VARIANCE; CELL SURVIVAL; CELLS, CULTURED; CHEMOKINE CCL3; CHEMOKINE CXCL12; DENTAL PULP; DENTITION, PERMANENT; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBROBLASTS; HUMANS; IN VITRO TECHNIQUES; PORPHYROMONAS GINGIVALIS; TIME FACTORS; TOOTH, DECIDUOUS;

EID: 84878168654     PISSN: 16787757     EISSN: 16787765     Source Type: Journal    
DOI: 10.1590/1678-7757201300004     Document Type: Article

References (41)

1. Abd-Elmeguid A, Yu DC, Kline LW, Moqbel R, Vliagoftis H. Dentin matrix protein-1 activates dental pulp fibroblasts. J Endod. 2012;38(1):75-80.
2. Ara T, Kurata K, Hirai K, Uchihashi T, Uematsu T, Imamura Y, et al. Human gingival fibroblasts are critical in sustaining inflammation in periodontal disease. J Periodontal Res. 2009;44(1):21-27.
3. Bonecchi R, Polentarutti N, Luini W, Borsatti A, Bernasconi S, Locati M, et al. Up-regulation of CCR1 and CCR3 and induction of chemotaxis to CC chemokines by IFN-gamma in human neutrophils. J Immunol. 1999;162(1):474-479.
4. Brouty-Boye D, Pottin-Clemenceau C, Doucet C, Jasmin C, Azzarone B. Chemokines and CD40 expression in human fibroblasts. EurJ Immunol. 2000;30(3):914-919.
5. Chiego Jr. D. Histology of the pulp. In: Avery J, editor. Oral development and histology. 3rd ed. ed. Stuttgart: Georg Thieme Verlag; 2002. p. 190-212.
6. Chu SC, Tsai CH, Yang SF, Huang FM, Su YF, Hsieh YS, et al. Induction of vascular endothelial growth factor gene expression by proinflammatory cytokines in human pulp and gingival fibroblasts. J Endod. 2004;30(10):704-707.
7. Enzerink A, Salmenpera P, Kankuri E, Vaheri A. Clustering of fibroblasts induces proinflammatory chemokine secretion promoting leukocyte migration. Mol Immunol. 2009;46(8- 9):1787-1795.
8. Farges JC, Keller JF, Carrouel F, Durand SH, Romeas A, Bleicher F, et al. Odontoblasts in the dental pulp immune response. J Exp Zool B Mol Dev Evol. 2009;312B(5):425-436.
9. Faria Morandini AC, Chaves Souza PP, Ramos-Junior ES, Brozoski DT, Sipert CR, Souza Costa CA, et al. Toll-like receptor 2 knockdown modulates interleukin (IL)-6 and IL-8 but not stromal derived factor-1 (SDF-1/CXCL12) in human periodontal ligament and gingival fibroblasts. J Periodontol. 2013;84(4):535-544.
10. Fedyk ER, Jones D, Critchley HO, Phipps RP, Blieden TM, Springer TA. Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing. J Immunol. 2001;166(9):5749-5754.
11. Ferreira DC, Brito DG, Cavalcanti BN. Cytokine production from human primary teeth pulp fibroblasts stimulated by different pulpotomy agents. J Dent Child (Chic). 2009;76(3):194-8.
12. Ferreira DC, Rocas IN, Paiva SS, Carmo FL, Cavalcante FS, Rosado AS, et al. Viral-bacterial associations in acute apical abscesses. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112(2):264-271.
13. Ghosh A, Park JY, Fenno C, Kapila YL. Porphyromonas gingivalis, gamma interferon, and a proapoptotic fibronectin matrix form a synergistic trio that induces c-Jun N-terminal kinase 1-mediated nitric oxide generation and cell death. Infect Immun. 2008;76(12):5514-5523.
14. Hatakeyama J, Tamai R, Sugiyama A, Akashi S, Sugawara S, Takada H. Contrasting responses of human gingival and periodontal ligament fibroblasts to bacterial cell-surface components through the CD14/Toll-like receptor system. Oral Microbiol Immunol. 2003;18(1):14-23.
15. Hennessy EJ, Parker AE, O'Neill LA. Targeting Toll-like receptors: emerging therapeutics? Nat Rev Drug Discov. 2010;9(4):293-307.
16. Hirao K, Yumoto H, Takahashi K, Mukai K, Nakanishi T, Matsuo T. Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts. J Dent Res. 2009;88(8):762-767.
17. Hosokawa Y, Hosokawa I, Ozaki K, Nakae H, Murakami K, Miyake Y, et al. CXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal disease. Clin Exp Immunol. 2005;141(3):467-474.
18. Jacinto RC, Gomes BP, Shah HN, Ferraz CC, Zaia AA, Souza-Filho FJ. Incidence and antimicrobial susceptibility of Porphyromonas gingivalis isolated from mixed endodontic infections. Int Endod J. 2006;39(1):62-70.
19. Jin MS, Kim SE, Heo JY, Lee ME, Kim HM, Paik SG, et al. Crystal structure of the TLR1-TLR2 heterodimer induced by binding of a tri-acylated lipopeptide. Cell. 2007;130(6):1071-1082.
20. Karin N. The multiple faces of CXCL12 (SDF-1alpha) in the regulation of immunity during health and disease. J Leukoc Biol. 2010;88(3):463-473.
21. Koga T, Nishihara T, Fujiwara T, Nisizawa T, Okahashi N, Noguchi T, et al. Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli. Infect Immun. 1985;47(3):638-647.
22. Kuo MY, Lan WH, Lin SK, Tsai KS, Hahn LJ. Collagen gene expression in human dental pulp cell cultures. Arch Oral Biol. 1992;37(11):945-952.
23. Love RM, Jenkinson HF. Invasion of dentinal tubules by oral bacteria. Crit Rev Oral Biol Med. 2002;13(2):171-183.
24. Mahanonda R, Sa-Ard-Iam N, Montreekachon P, Pimkhaokham A, Yongvanichit K, Fukuda MM, et al. IL-8 and IDO Expression by Human Gingival Fibroblasts via TLRs. J Immunol. 2007;178(2):1151-1157.
25. Martinez EF, Araujo VC. In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts. Int Endod J. 2004;37(11):749-755.
26. Maurer M, von Stebut E. Macrophage inflammatory protein-1. Int J Biochem Cell Biol. 2004;36(10):1882-1886.
27. Morandini AC, Sipert CR, Gasparoto TH, Greghi SL, Passanezi E, Rezende ML, et al. Differential production of macrophage inflammatory protein-1alpha, stromal-derived factor-1, and IL-6 by human cultured periodontal ligament and gingival fibroblasts challenged with lipopolysaccharide from P. gingivalis. J Periodontol. 2010;81(2):310-317.
28. Morandini AC, Sipert CR, Ramos-Junior ES, Brozoski DT, Santos CF. Periodontal ligament and gingival fibroblasts participate in the production of TGF-beta, interleukin (IL)-8 and IL-10. Braz Oral Res. 2011;25(2):157-162.
29. Ricart BG, John B, Lee D, Hunter CA, Hammer DA. Dendritic cells distinguish individual chemokine signals through CCR7 and CXCR4. J Immunol. 2011;186(1):53-61.
30. Ronnov-Jessen L, Celis JE, Van Deurs B, Petersen OW. A fibroblast-associated antigen: characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells. J Histochem Cytochem. 1992;40(4):475-486.
31. Schall TJ, Bacon K, Camp RD, Kaspari JW, Goeddel DV. Human macrophage inflammatory protein alpha (MIP-1 alpha) and MIP-1 beta chemokines attract distinct populations of lymphocytes. J Exp Med. 1993;177(6):1821-1826.
32. Scheres N, Laine ML, de Vries TJ, Everts V, van Winkelhoff AJ. Gingival and periodontal ligament fibroblasts differ in their inflammatory response to viable Porphyromonas gingivalis. J Periodontal Res. 2010;45(2):262-270.
33. Sipert CR, Moraes IG, Bernardinelli N, Garcia RB, Bramante CM, Gasparoto TH, et al. Heat-killed Enterococcus faecalis alters nitric oxide and CXCL12 production but not CXCL8 and CCL3 production by cultured human dental pulp fibroblasts. J Endod. 2010;36(1):91-94.
34. Souza PP, Palmqvist P, Lundgren I, Lie A, Costa-Neto CM, Lundberg P, et al. Stimulation of IL-6 cytokines in fibroblasts by toll-like receptors 2. J Dent Res. 2010;89(8):802-7.
35. Sundqvist G, Johansson E, Sjogren U. Prevalence of black- pigmented bacteroides species in root canal infections. J Endod. 1989;15(1):13-19.
36. van Winkelhoff AJ, Carlee AW, de Graaff J. Bacteroides endodontalis and other black-pigmented Bacteroides species in odontogenic abscesses. Infect Immun. 1985;49(3):494-497.
37. Wang PL, Azuma Y, Shinohara M, Ohura K. Toll-like receptor 4 mediated signal pathway induced by Porphyromonas gingivalis lipopolysaccharide in human gingival fibroblasts. Biochem Biophys Res Commun. 2000;273(3):1161-1167.
38. Wang PL, Shirasu S, Shinohar M, Azuma Y, Daito M, Yasuda H, et al. IL-10 inhibits Porphyromonas gingivalis LPS-stimulated human gingival fibroblasts production of IL-6. Biochem Biophys Res Commun. 1999;263(2):372-377.
39. Yang LC, Huang FM, Lin CS, Liu CM, Lai CC, Chang YC. Induction of interleukin-8 gene expression by black-pigmented Bacteroides in human pulp fibroblasts and osteoblasts. Int Endod J. 2003;36(11):774-779.
40. Yang LC, Tsai CH, Huang FM, Liu CM, Lai CC, Chang YC. Induction of interleukin-6 gene expression by pro-inflammatory cytokines and black-pigmented Bacteroides in human pulp cell cultures. Int Endod J. 2003;36(5):352-357.
41. Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity. Immunity. 2000;12(2):121-127.