Expression of recombinant human complement C1q allows identification of the C1r/C1s-binding sites

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 21, 2013, Pages 8650-8655

  Bally, Isabelle ^a,b,c   Ancelet, Sarah ^a,b,c   Moriscot, Christine ^a,b,c   Gonnet, Florence ^d,e   Mantovani, Alberto ^f   Daniel, Régis ^d,e   Schoehn, Guy ^a,b,c   Arlaud, Gérard J ^a,b,c   Thielens, Nicole M ^a,b,c  

^a INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^b CNRS   (France)

^c UNIV GRENOBLE ALPES   (France)

^d Centre National de la Recherche Scientifique   (France)

^e UNIVERSITÉ D EVRY VAL D ESSONNE   (France)

^f HUMANITAS CLINICAL AND RESEARCH CENTER   (Italy)

Author keywords

C1 complex; Innate immunity; Protein engineering

Indexed keywords

BONE MORPHOGENETIC PROTEIN; CALCIUM; COLLAGEN; COMPLEMENT COMPONENT C1Q; COMPLEMENT COMPONENT C1R; COMPLEMENT COMPONENT C1S; COMPLEMENT COMPONENT UEGF; IMMUNOGLOBULIN G; LYSINE; PENTRAXIN 3; POLYPEPTIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL STRAIN HEK293; CHEMICAL ANALYSIS; CONTROLLED STUDY; EMBRYO; FLOWER; GENE MUTATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LIGAND BINDING; MAMMAL CELL; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TARGETING; STRUCTURE ANALYSIS; SURFACE PLASMON RESONANCE; WESTERN BLOTTING;

C1 COMPLEX; INNATE IMMUNITY; PROTEIN ENGINEERING;

AMINO ACID SUBSTITUTION; BINDING SITES; C-REACTIVE PROTEIN; CALCIUM; COMPLEMENT ACTIVATION; COMPLEMENT C1Q; COMPLEMENT C1R; COMPLEMENT C1S; GENE EXPRESSION; HEK293 CELLS; HUMANS; IMMUNOGLOBULIN G; MUTATION, MISSENSE; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SERUM AMYLOID P-COMPONENT; SURFACE PLASMON RESONANCE;

EID: 84878163798     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1304894110     Document Type: Article

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