Curcumin attenuates cyclooxygenase-2 expression via inhibition of the NF-κb pathway in lipopolysaccharide-stimulated human gingival fibroblasts

Cell Biology International

Volumn 37, Issue 5, 2013, Pages 443-448

  Hu, Ping ^a   Huang, Ping ^a   Chen, Min Wei ^a  

^a TONGJI HOSPITAL OF TONGJI MEDICAL COLLEGE OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Curcumin; Cyclooxygenase 2; Nuclear factor kappa B; Periodontitis; Porphyromonas gingivalis; Quantitative realtime polymerase chain reaction

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; CURCUMIN; CYCLOOXYGENASE 2; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LUCIFERASE; MESSENGER RNA;

ANTIINFLAMMATORY ACTIVITY; ARTICLE; CELL STIMULATION; CONTROLLED STUDY; DOWN REGULATION; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME INHIBITION; FIBROBLAST; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; GINGIVA; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; PERIODONTITIS; PORPHYROMONAS GINGIVALIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS INHIBITION; REAL TIME POLYMERASE CHAIN REACTION; RNA SYNTHESIS; TRANSCRIPTION INITIATION; WESTERN BLOTTING;

EID: 84878143333     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1002/cbin.10050     Document Type: Article

References (24)

1. Ara T, Maeda Y, Fujinami Y, Imamura Y, Hattori T, Wang PL (2008) Preventive effects of a Kampo medicine, Shosaikoto, on inflammatory responses in LPS-treated human gingival fibroblasts. Biol Pharm Bull 31: 1141-4.
2. Bowie A, O'Neill LA (2000) Oxidative stress and nuclear factorkappaB activation: a reassessment of the evidence in the light of recent discoveries. Biochem Pharmacol 59: 13-23.
3. Chen D, Nie M, Fan MW, Bian Z (2008) Anti-inflammatory activity of curcumin in macrophages stimulated by lipopolysaccharides from Porphyromonas gingivalis. Pharmacology 82: 264-9.
4. Choi H, Lim W, Kim I, Kim J, Ko Y, Kwon H, Kim S, Kabir KM, Li X, Kim O, Lee Y, Kim S, Kim O (2012) Inflammatory cytokines are suppressed by light-emitting diode irradiation of P. gingivalis LPS-treated human gingival fibroblasts: inflammatory cytokine changes by LED irradiation. Lasers Med Sci 27: 459-67.
5. Darveau RP (2009) The oral microbial consortium's interaction with the periodontal innate defense system. DNA Cell Biol 28: 389-95.
6. Das L, VinayakM, (2012) Anti-carcinogenic action of curcumin by activation of antioxidant defence system and inhibition of NF-kB signalling in lymphoma-bearing mice. Biosci Rep 32: 161-70.
7. Deng Y, Zhao K, Zhang XF, Hu P, Chen GQ (2002) Study on the three-dimensional proliferation of rabbit articular cartilagederived chondrocytes on polyhydroxyalkanoate scaffolds. Biomaterials 23: 4049-56.
8. Esatbeyoglu T, Huebbe P, Ernst IM, Chin D,Wagner AE, Rimbach G (2012) Curcumin-from molecule to biological function. Angew Chem Int Ed Engl 51: 5308-32.
9. Feng L, Xia Y, Garcia GE, Hwang D, Wilson CB (1995) Involvement of reactive oxygen intermediates in cyclooxygenase-2 expression induced by interleukin-1, tumor necrosis factor-alpha, and lipopolysaccharide. J Clin Invest 95: 1669-75.
10. Guimarães MR, Coimbra LS, de Aquino SG, Spolidorio LC, Kirkwood KL, Rossa C Jr (2011) Potent anti-inflammatory effects of systemically administered curcumin modulate periodontal disease in vivo. J Periodontal Res 46: 269-79.
11. Hofer TP, Bitterle E, Beck-Speier I, Maier KL, Frankenberger M, Heyder J, Ziegler-Heitbrock L (2004) Diesel exhaust particles increase LPS-stimulated COX-2 expression and PGE2 production in human monocytes. J Leukoc Biol 75: 856-64.
12. Kwak HB, Sun HM, Ha H, KimHN, Lee JH, Kim HH, Shin HI, Lee ZH (2008) Tanshinone IIA suppresses inflammatory bone loss by inhibiting the synthesis of prostaglandin E2 in osteoblasts. Eur J Pharmacol 601: 30-7.
13. Lamont RJ, Jenkinson HF (1998) Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis. Microbiol Mol Biol Rev 62: 1244-63.
14. Liu Y, Bridges R, Wortham A, Kulesz-Martin M (2012) NF-kB repression by PIAS3 mediated RelA SUMOylation. PLoS ONE 7: e37636.
15. Mitchell JA, Larkin S, Williams TJ (1995) Cyclooxygenase-2: regulation and relevance in inflammation. Biochem Pharmacol 50: 1535-42.
16. Noguchi K, Shitashige M, Yanai M, Morita I, Nishihara T, Murota S, Ishikawa I (1996) Prostaglandin production via induction of cyclooxygenase-2 by human gingival fibroblasts stimulated with lipopolysaccharides. Inflammation 20: 555-68.
17. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3: 1101-8.
18. Stancovski I, Baltimore D (1997) NF-kappaB activation: the I kappaB kinase revealed? Cell 91: 299-302
19. Vane JR, Bakhle YS, Botting RM (1998) Cyclooxygenases 1 and 2. Annu Rev Pharmacol Toxicol 38: 97-120.
20. vonMetzler I, Krebbel H, Kuckelkorn U, Heider U, Jakob C, Kaiser M, Fleissner C, Terpos E, Sezer O (2009) Curcumin diminishes human osteoclastogenesis by inhibition of the signalosomeassociated I kappaB kinase. J Cancer Res Clin Oncol 135: 173-9.
21. Wang PL, Ohura K (2002) Porphyromonas gingivalis lipopolysaccharide signaling in gingival fibroblasts-CD14 and Toll-like receptors. Crit Rev Oral Biol Med 13: 132-42.
22. Williams CS, Mann M, DuBois RN (1999) The role of cyclooxygenases in inflammation, cancer, and development Oncogene 18: 7908-16
23. Yamamoto K, Arakawa T, Ueda N, Yamamoto S (1995) Transcriptional roles of nuclear factor kappa B and nuclear factor-interleukin-6 in the tumor necrosis factor alphadependent induction of cyclooxygenase-2 in MC3T3-E1 cells. J Biol Chem 270: 31315-20.
24. Zhou T, Chen D, Li Q, Sun X, Song Y, Wang C (2012) Curcumin inhibits inflammatory response and bone loss during experimental periodontitis in rats. Acta Odontol Scand 4: 1-8.