Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp. P7 with unusual thermostability characteristics

Enzyme and Microbial Technology

Volumn 53, Issue 1, 2013, Pages 18-24

  Florczak, Tomasz ^a,b   Daroch, Maurycy ^b,c   Wilkinson, Mark Charles ^b   Białkowska, Aneta ^a   Bates, Andrew Derek ^b   Turkiewicz, Marianna ^a   Iwanejko, Lesley Ann ^b  

^a LODZ UNIVERSITY OF TECHNOLOGY   (Poland)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

^c PEKING UNIVERSITY   (China)

Author keywords

Cold adaptation; Inverse PCR; Lipase; Psychrophillic enzyme; Thermostability

Indexed keywords

454-PYROSEQUENCING; COLD ADAPTATION; COLD-ADAPTED LIPASE; ENANTIOSELECTIVE TRANSESTERIFICATION; INDUSTRIAL POTENTIALS; INVERSE-PCR; SECONDARY ALCOHOLS; THERMOSTABILITY;

CLONING; ENANTIOSELECTIVITY; LIPASES; PROTEINS; PURIFICATION;

YEAST;

FUNGAL PROTEIN; LIPG7 PROTEIN; TRANSCRIPTOME; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN;

ADAPTATION; ARTICLE; BIOCATALYST; CLONING; COLD; DENATURATION; ENANTIOSELECTIVITY; FUNGUS; GEOMYCES; HEATING; INVERSE POLYMERASE CHAIN REACTION; NONHUMAN; PH; PROTEIN PURIFICATION; PYROSEQUENCING; SACCHAROMYCES CEREVISIAE; TEMPERATURE SENSITIVE MUTANT; THERMOSTABILITY; ANTARCTICA; ASCOMYCETES; BIOTECHNOLOGY; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PROCEDURES; STEREOISOMERISM;

FUNGI; GEOMYCES; SACCHAROMYCES CEREVISIAE;

ADAPTATION, PHYSIOLOGICAL; ANTARCTIC REGIONS; ASCOMYCOTA; BACTERIAL PROTEINS; BIOTECHNOLOGY; CLONING, MOLECULAR; COLD TEMPERATURE; ENZYME STABILITY; FUNGI; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; STEREOISOMERISM;

EID: 84878019762     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2013.03.021     Document Type: Article

References (45)

1. Hasan F., Shah A.A., Hameed A. Industrial applications of microbial lipases. Enzyme and Microbial Technology 2006, 39:235-251.
2. Bornscheuer U.T. Microbial carboxyl esterases: classification, properties and application in biocatalysis. FEMS Microbiology Review 2002, 26:73-81.
3. Gotor-Fernandez V., Brieva R., Gotor V. Lipases: useful biocatalysts for the preparation of pharmaceuticals. Journal of Molecular Catalysis B: Enzymatic 2006, 40:111-120.
4. Antczak M.S., Kubiak A., Antczak T., Bielecki S. Enzymatic biodiesel synthesis - key factors affecting efficiency of the process. Renewable Energy 2009, 34:1185-1194.
5. Messaoudi A., Belguith H., Gram I., Ben Hamida J. Classification of EC 3.1.1, 3 bacterial true lipases using phylogenetic analysis. African Journal of Biotechnology 2010, 9:8243-8247.
6. Adams M.W.W., Perler F.B., Kelly R.M. Extremozymes expanding the limits of biocatalysis. Nature Biotechnology 1995, 13:662-668.
7. Bruins M., Janssen A., Boom R. Thermozymes and their applications. Applied Biochemistry and Biotechnology 2001, 90:155-186.
8. Cavicchioli R., Siddiqui K.S., Andrews D., Sowers K.R. Low-temperature extremophiles and their applications. Current Opinion in Biotechnology 2002, 13:253-261.
9. Overbeeke P.L.A., Ottosson J., Hult K., Jongejan J.A., Duine J.A. The temperature dependence of enzymatic kinetic resolutions reveals the relative contribution of enthalpy and entropy to enzymatic enantioselectivity. Biocatalysis and Biotransformation 1999, 17:61-79.
10. Anderson E.M., Larsson K.M., Kirk O. One biocatalyst-many applications: the use of Candida antarctica B-lipase in organic synthesis. Biocatalysis and Biotransformation 1998, 16:181-204.
11. Joseph B., Ramteke P.W., Thomas G., Shirivastava N. Cold-active microbial lipases: a versatile tool for industrial applications. Biotechnology and Molecular Biology Reviews 2007, 2:39-48.
12. Fickers P., Marty A., Nicaud J.M. The lipases from Yarrowia lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications. Biotechnology Advances 2011, 29:632-644.
13. Mateo C., Palomo J.M., Fernandez-Lorente G., Guisan J.M., Fernandez-Lafuente R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme and Microbial Technology 2007, 40:1451-1463.
14. Cao L. Immobilised enzymes: science or art. Current Opinion in Chemical Biology 2005, 9:217-226.
15. Lockhart R.J., Van Dyke M.I., Beadle I.R., Humphreys P., McCarthy A.J. Molecular biological detection of anaerobic gut fungi (Neocallimastigales) from landfill sites. Applied and Environmental Microbiology 2006, 72:5659-5661.
16. Vakhlu J., Johri S., Verma V., Koul S., Parshad R., Taneja S.C., et al. Purification and properties of enantioselective ester hydrolase from a strain of Trichosporon species (DSMZ 11829). Enzyme and Microbial Technology 2005, 37:330-339.
17. Szczesna-Antczak M., Antczak T., Piotrowicz-Wasiak M., Rzyska M., Binkowska N., Bielecki S. Relationships between lipases and lipids in mycelia of two Mucor strains. Enzyme and Microbial Technology 2006, 39:1214-1222.
18. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
19. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 1976, 72:248-254.
20. Artimo P., Jonnalagedda M., Arnold K., Baratin D., Csardi G., de Castro E., et al. ExPASy: SIB bioinformatics resource portal. Nucleic Acids Research 2012, 40:W597-W603.
21. Chomczynski P., Sacchi N. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nature Protocols 2006, 1:581-585.
22. Ochman H., Gerber A.S., Hartl D.L. Genetic applications of an inverse polymerase chain reaction. Genetics 1988, 120:621-623.
23. Gietz R.D., Schiestl R.H. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nature Protocols 2007, 2:31-34.
24. Casas-Godoy L., Duquesne S., Bordes F., Sandoval G., Marty A. Lipases: an overview. Methods in Molecular Biology 2012, 861:3-30.
25. Fojan P., Jonson P.H., Petersen M.T.N., Petersen S.B. What distinguishes an esterase from a lipase: a novel structural approach. Biochimie 2000, 82:1033-1041.
26. Tsujita T., Shirai K., Saito Y., Okuda H. Relationship between lipase and esterase. Progress in Clinical and Biological Research 1990, 344:915-933.
27. Ali Y.B., Verger R., Abousalham A. Lipases or esterases: does it really matter? Toward a new bio-physico-chemical classification. Methods in Molecular Biology 2012, 861:31-51.
28. Feller G., Gerday C. Psychrophilic enzymes: hot topics in cold adaptation. Nature Reviews Microbiology 2003, 1:200-208.
29. Joseph B., Ramteke P.W., Thomas G. Cold active microbial lipases: some hot issues and recent developments. Biotechnology Advances 2008, 26:457-470.
30. Eddine A.N., Hannemann F., Schafer W. Cloning and expression analysis of NhL1, a gene encoding an extracellular lipase from the fungal pea pathogen Nectria haematococca MP VI (Fusarium solani f. sp pisi) that is expressed in planta. Molecular Genetics and Genomics 2001, 265:215-224.
31. Elend C., Schmeisser C., Hoebenreich H., Steele H.L., Streit W.R. Isolation and characterization of a metagenome-derived and cold-active lipase with high stereospecificity for (R)-ibuprofen esters. Journal of Biotechnology 2007, 130:370-377.
32. Zhang J.W., Zeng R.Y. Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp. 7323. Marine Biotechnology 2008, 10:612-621.
33. Ryu H.S., Kim H.K., Choi W.C., Kim M.H., Park S.Y., Han N.S., et al. New cold-adapted lipase from Photobacterium lipolyticum sp. nov that is closely related to filamentous fungal lipases. Applied Microbiology and Biotechnology 2006, 70:321-326.
34. Fickers P., Ongena M., Destain J., Weekers F., Thonart P. Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica. Enzyme and Microbial Technology 2006, 38:756-759.
35. Cai Y.J., Wang L., Liao X.R., Ding Y.R., Sun J. Purification and partial characterization of two new cold-adapted lipases from mesophilic Geotrichum sp. SYBC WU-3. Process Biochemistry 2009, 44:786-790.
36. Tripathi M.K., Roy U., Jinwal U.K., Jain S.K., Roy P.K. Cloning, sequencing and structural features of a novel Streptococcus lipase. Enzyme and Microbial Technology 2004, 34:437-445.
37. Parra L.P., Reyes F., Acevedo J.P., Salazar O., Andrews B.A., Asenjo J.A. Cloning and fusion expression of a cold-active lipase from marine Antarctic origin. Enzyme and Microbial Technology 2008, 42:371-377.
38. Al Khudary R., Venkatachalam R., Katzer M., Elleuche S., Antranikian G. A cold-adapted esterase of a novel marine isolate, Pseudoalteromonas arctica: gene cloning, enzyme purification and characterization. Extremophiles 2010, 14:273-285.
39. Yuan B.H., Cai Y.J., Liao X.R., Yun L.H., Zhang F., Zhang D.B. Isolation and identification of a cold-adapted lipase producing strain from decayed seeds of Ginkgo biloba L. and characterization of the lipase. African Journal of Biotechnology 2010, 9:2661-2667.
40. Novototskaya-Vlasova K., Petrovskaya L., Yakimov S., Gilichinsky D. Cloning, purification, and characterization of a cold-adapted esterase produced by Psychrobacter cryohalolentis K5T from Siberian cryopeg. FEMS Microbiology Ecology 2012, 82:367-375.
41. Sharma R., Chisti Y., Banerjee U.C. Production, purification, characterization, and applications of lipases. Biotechnology Advances 2001, 19:627-662.
42. Cheng Y.Y., Qian Y.K., Li Z.F., Wu Z.H., Liu H., Li Y.Z. A novel cold-adapted lipase from Sorangium cellulosum strain So0157-2: gene cloning, expression, and enzymatic characterization. International Journal of Molecular Sciences 2011, 12:6765-6780.
43. Yadav K.N., Adsul M.G., Bastawde K.B., Jadhav D.D., Thulasiram H.V., Gokhale D.V. Differential induction, purification and characterization of cold active lipase from Yarrowia lipolytica NCIM 3639. Bioresource Technology 2011, 102:10663-10670.
44. Zhang N.Y., Suen W.C., Windsor W., Xiao L., Madison V., Zaks A. Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution. Protein Engineering 2003, 16:599-605.
45. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J.H., Zhang Z., Miller W., et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 1997, 25:3389-3402.