Depletion of polycistronic transcripts using short interfering RNAs: CDNA synthesis method affects levels of non-targeted genes determined by quantitative PCR

Virology Journal

Volumn 10, Issue , 2013, Pages

  Hanning, Jennifer E ^a   Groves, Ian J ^a   Pett, Mark R ^a   Coleman, Nicholas ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

cDNA synthesis; Quantitative PCR; Short interfering RNA

Indexed keywords

COMPLEMENTARY DNA; HEXAMER PRIMER; MESSENGER RNA; OLIGO DT PRIMER; PRIMER DNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CELL LINE; COMPARATIVE STUDY; DNA SYNTHESIS; GENE; HUMAN; HUMAN CELL; HUMAN PAPILLOMAVIRUS TYPE 16; KERATINOCYTE; POLYCISTRONIC TRANSCRIPTS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; SQUAMOUS CELL CARCINOMA; VIRUS GENE;

DNA, COMPLEMENTARY; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, VIRAL; HUMAN PAPILLOMAVIRUS 16; HUMANS; KERATINOCYTES; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA, VIRAL;

HUMAN PAPILLOMAVIRUS;

EID: 84877880997     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-10-159     Document Type: Article

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