A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila

Development (Cambridge)

Volumn 140, Issue 11, 2012, Pages 2434-2442

  Bischof, Johannes ^a   Björklund, Mikael ^b,c   Furger, Edy ^a   Schertel, Claus ^a   Taipale, Jussi ^c,d   Basler, Konrad ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

^c UNIVERSITY OF HELSINKI   (Finland)

^d KAROLINSKA INSTITUTE   (Sweden)

Author keywords

C31; Drosophila; ORFeome library; Overexpression

Indexed keywords

EPITOPE;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA BARCODING; DROSOPHILA; EMBRYO; FEMALE; GENE LIBRARY; IN VIVO STUDY; MALE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; STOP CODON; TRANSGENIC ANIMAL;

ΦC31; DROSOPHILA; ORFEOME LIBRARY; OVEREXPRESSION;

ANIMALS; DNA BARCODING, TAXONOMIC; DROSOPHILA; DROSOPHILA PROTEINS; EPITOPES; GENE LIBRARY; GENETIC TECHNIQUES; OPEN READING FRAMES; PLASMIDS; TRANSGENES;

EID: 84877727235     PISSN: 09501991     EISSN: 14779129     Source Type: Journal    
DOI: 10.1242/dev.088757     Document Type: Article

References (39)

1. Basler, K. and Struhl, G. (1994). Compartment boundaries and the control of Drosophila limb pattern by hedgehog protein. Nature 368, 208-214.
2. Bateman, J. R., Lee, A. M. and Wu, C. T. (2006). Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics 173, 769-777.
3. Bellen, H. J., Levis, R. W., He, Y., Carlson, J. W., Evans-Holm, M., Bae, E., Kim, J., Metaxakis, A., Savakis, C., Schulze, K. L. et al. (2011). The Drosophila gene disruption project: progress using transposons with distinctive site specificities. Genetics 188, 731-743.
4. Bischof, J., Maeda, R. K., Hediger, M., Karch, F. and Basler, K. (2007). An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proc. Natl. Acad. Sci. USA 104, 3312-3317.
5. Björklund, M., Taipale, M., Varjosalo, M., Saharinen, J., Lahdenperä, J. and Taipale, J. (2006). Identification of pathways regulating cell size and cell-cycle progression by RNAi. Nature 439, 1009-1013.
6. Brand, A. H. and Perrimon, N. (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401-415.
7. Brothers, S. P., Janovick, J. A. and Conn, P. M. (2003). Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J. Clin. Endocrinol. Metab. 88, 6107-6112.
8. Caussinus, E., Kanca, O. and Affolter, M. (2011). Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Nat. Struct. Mol. Biol. 19, 117-121.
9. Cavener, D. R. (1987). Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates. Nucleic Acids Res. 15, 1353-1361.
10. Dietzl, G., Chen, D., Schnorrer, F., Su, K. C., Barinova, Y., Fellner, M., Gasser, B., Kinsey, K., Oppel, S., Scheiblauer, S. et al. (2007). A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448, 151-156.
11. Groth, A. C., Fish, M., Nusse, R. and Calos, M. P. (2004). Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 166, 1775-1782.
12. Herskowitz, I. (1987). Functional inactivation of genes by dominant negative mutations. Nature 329, 219-222.
13. Horn, C., Jaunich, B. and Wimmer, E. A. (2000). Highly sensitive, fluorescent transformation marker for Drosophila transgenesis. Dev. Genes Evol. 210, 623-629.
14. Hu, C. D., Chinenov, Y. and Kerppola, T. K. (2002). Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol. Cell 9, 789-798.
15. Jankovics, F. and Brunner, D. (2006). Transiently reorganized microtubules are essential for zippering during dorsal closure in Drosophila melanogaster. Dev. Cell 11, 375-385.
16. Jarvik, J. W. and Telmer, C. A. (1998). Epitope tagging. Annu. Rev. Genet. 32, 601-618.
17. Jaw, T. J., You, L. R., Knoepfler, P. S., Yao, L. C., Pai, C. Y., Tang, C. Y., Chang, L. P., Berthelsen, J., Blasi, F., Kamps, M. P. et al. (2000). Direct interaction of two homeoproteins, homothorax and extradenticle, is essential for EXD nuclear localization and function. Mech. Dev. 91, 279-291.
18. Lai, S. L. and Lee, T. (2006). Genetic mosaic with dual binary transcriptional systems in Drosophila. Nat. Neurosci. 9, 703-709.
19. Larsen, C., Franch-Marro, X., Hartenstein, V., Alexandre, C. and Vincent, J. P. (2006). An efficient promoter trap for detection of patterned gene expression and subsequent functional analysis in Drosophila. Proc. Natl. Acad. Sci. USA 103, 17813-17817.
20. Lee, T. and Luo, L. (1999). Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451-461.
21. Miklos, G. L. and Rubin, G. M. (1996). The role of the genome project in determining gene function: insights from model organisms. Cell 86, 521-529.
22. Parks, A. L., Cook, K. R., Belvin, M., Dompe, N. A., Fawcett, R., Huppert, K., Tan, L. R., Winter, C. G., Bogart, K. P., Deal, J. E. et al. (2004). Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. Nat. Genet. 36, 288-292.
23. Prelich, G. (2012). Gene overexpression: uses, mechanisms, and interpretation. Genetics 190, 841-854.
24. Ramani, A. K., Chuluunbaatar, T., Verster, A. J., Na, H., Vu, V., Pelte, N., Wannissorn, N., Jiao, A. and Fraser, A. G. (2012). The majority of animal genes are required for wild-type fitness. Cell 148, 792-802.
25. Romano, J. D., Schmidt, W. K. and Michaelis, S. (1998). The Saccharomyces cerevisiae prenylcysteine carboxyl methyltransferase Ste14p is in the endoplasmic reticulum membrane. Mol. Biol. Cell 9, 2231-2247.
26. Rørth, P. (1996). A modular misexpression screen in Drosophila detecting tissuespecific phenotypes. Proc. Natl. Acad. Sci. USA 93, 12418-12422.
27. Saka, Y., Hagemann, A. I., Piepenburg, O. and Smith, J. C. (2007). Nuclear accumulation of Smad complexes occurs only after the midblastula transition in Xenopus. Development 134, 4209-4218.
28. Schertel, C., Huang, D., Björklund, M., Bischof, J., Yin, D., Li, R., Wu, Y., Zeng, R., Wu, J., Taipale, J. et al. (2013). Systematic screening of a Drosophila ORF library in vivo uncovers Wnt/Wg pathway components. Dev. Cell 25, 207-219.
29. Schlake, T. and Bode, J. (1994). Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 33, 12746-12751.
30. Taxis, C., Stier, G., Spadaccini, R. and Knop, M. (2009). Efficient protein depletion by genetically controlled deprotection of a dormant N-degron. Mol. Syst. Biol. 5, 267.
31. Venken, K. J., He, Y., Hoskins, R. A. and Bellen, H. J. (2006). P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster. Science 314, 1747-1751.
32. Venken, K. J., Simpson, J. H. and Bellen, H. J. (2011). Genetic manipulation of genes and cells in the nervous system of the fruit fly. Neuron 72, 202-230.
33. Viktorinová, I. and Wimmer, E. A. (2007). Comparative analysis of binary expression systems for directed gene expression in transgenic insects. Insect. Biochem. Mol. Biol. 37, 246-254.
34. Wei, N., Serino, G. and Deng, X. W. (2008). The COP9 signalosome: more than a protease. Trends Biochem. Sci. 33, 592-600.
35. Wittwer, F., Jaquenoud, M., Brogiolo, W., Zarske, M., Wüstemann, P., Fernandez, R., Stocker, H., Wymann, M. P. and Hafen, E. (2005). Susi, a negative regulator of Drosophila PI3-kinase. Dev. Cell 8, 817-827.
36. Xu, R., Deng, K., Zhu, Y., Wu, Y., Ren, J., Wan, M., Zhao, S., Wu, X., Han, M., Zhuang, Y. et al. (2008). A large-scale functional approach to uncover human genes and pathways in Drosophila. Cell Res. 18, 1114-1127.
37. Yagi, R., Mayer, F. and Basler, K. (2010). Refined LexA transactivators and their use in combination with the Drosophila Gal4 system. Proc. Natl. Acad. Sci. USA 107, 16166-16171.
38. Yu, C., Wan, K. H., Hammonds, A. S., Stapleton, M., Carlson, J. W. and Celniker, S. E. (2011). Development of expression-ready constructs for generation of proteomic libraries. Methods Mol. Biol. 723, 257-272.
39. Zhong, J. and Yedvobnick, B. (2009). Targeted gain-of-function screening in Drosophila using GAL4-UAS and random transposon insertions. Genet. Res. (Camb.) 91, 243-258.