Design, recombinant expression and in vitro maturation of human insulin-like peptide 6 and a biotin-labeled analogue

Process Biochemistry

Volumn 46, Issue 6, 2011, Pages 1243-1247

  Luo, Xiao ^a   Huang, Chun Hua ^a   Shao, Xiao Xia ^a   Guo, Zhan Yun ^a  

^a TONGJI UNIVERSITY   (China)

Author keywords

Biotin labeling; INSL6; Insulin like peptide; Recombinant expression; Refolding

Indexed keywords

BENIGN PROSTATE HYPERPLASIA; BIOTIN LABELING; BIOTIN MOIETY; CYANOGEN BROMIDE; IN-VITRO; INSL6; N-TERMINALS; PEPTIDE HORMONES; PRECLINICAL STUDIES; RECOMBINANT EXPRESSION; REFOLDING;

BROMINE COMPOUNDS; CIRCULAR DICHROISM SPECTROSCOPY; COENZYMES; DICHROISM; ESCHERICHIA COLI; INSULIN;

PEPTIDES;

ESCHERICHIA COLI;

EID: 79955591158     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2011.02.011     Document Type: Article

References (10)

1. Shabanpoor F, Separovic F, Wade JD. The human insulin superfamily of polypeptide hormones. Vitam Horm 2009;80:1-31.
2. Hsu SY. Cloning of two novel mammalian paralogs of relaxin/insulin family proteins and their expression in testis and kidney. Mol Endocrinol 1999;13:2163-74. (Pubitemid 30637274)
3. Lok S, Johnston DS, Conklin D, Lofton-Day CE, Adams RL, Jelmberg AC, et al. Identification of INSL6, a new member of the insulin family that is expressed in the testis of the human and rat. Biol Reprod 2000;62:1593-9. (Pubitemid 30333740)
4. Kasik J, Muglia L, Stephan DA, Menon RK. Identification, chromosomal mapping, and partial characterization ofmouseInsI6: anewmemberof the insulin family. Endocrinology 2000;141:458-61. (Pubitemid 32260390)
5. Brailoiu GC, Dun SL, Yin D, Yang J, Chang JK, Dun NJ. Insulin-like 6 immunoreactivity in the mouse brain and testis. Brain Res 2005;1040:187-90. (Pubitemid 40459632)
6. Lu C, Walker WH, Sun J, Weisz OA, Gibbs RB, Witchel SF, et al. Insulin-like peptide 6: characterization of secretory status and posttranslational modifications. Endocrinology 2006;147:5611-23. (Pubitemid 44833478)
7. Burnicka-Turek O, Shirneshan K, Paprotta I, Grzmil P, Meinhardt A, Engel W, et al. Inactivation of insulin-like factor 6 disrupts the progression of spermatogenesis at late meiotic prophase. Endocrinology 2009;150:4348-57.
8. Luo X, Bathgate RA, Liu YL, Shao XX, Wade JD, Guo ZY. Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3. FEBS J 2009;276:5203-11.
9. Luo X, Bathgate RA, Zhang WJ, Liu YL, Shao XX, Wade JD, et al. Design and recombinant expression of insulin-like peptide 5 precursors and the preparation of mature human INSL5. Amino Acids 2010;39:1343-52.
10. Luo X, Liu YL, Layfield S, Shao XX, Bathgate RA, Wade JD, et al. A simple approach for the preparation of mature human relaxin-3. Peptides 2010;31:2083-8.