A simple approach for the preparation of mature human relaxin-3

Peptides

Volumn 31, Issue 11, 2010, Pages 2083-2088

  Luo, Xiao ^a   Liu, Ya Li ^b   Layfield, Sharon ^c   Shao, Xiao Xia ^a   Bathgate, Ross A D ^c   Wade, John D ^c   Guo, Zhan Yun ^a  

^a TONGJI UNIVERSITY   (China)

^b TONGJI UNIVERSITY SCHOOL OF MEDICINE   (China)

^c UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Insulin like peptide; Recombinant expression; Relaxin 3

Indexed keywords

DISULFIDE; INSULIN; METAL ION; RECOMBINANT PROTEIN; RELAXIN 3;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; ASSAY; CIRCULAR DICHROISM; COST; ESCHERICHIA COLI; HUMAN; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PURIFICATION; RECEPTOR BINDING; STEM CELL;

AMINO ACID SEQUENCE; BASE SEQUENCE; CIRCULAR DICHROISM; ESCHERICHIA COLI; HUMANS; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; PROTEIN PRECURSORS; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT PROTEINS; RELAXIN;

EID: 77957846474     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.peptides.2010.07.022     Document Type: Article

References (27)

1. A. Banerjee, P.J. Shen, S. Ma, R.A. Bathgate, and A.L. Gundlach Swim stress excitation of nucleus incertus and rapid induction of relaxin-3 expression via CRF1 activation Neuropharmacology 58 2010 145 155
2. R.A. Bathgate, F. Lin, N.F. Hanson, L. Otvos Jr., A. Guidolin, and C. Giannakis Relaxin-3: improved synthesis strategy and demonstration of its high-affinity interaction with the relaxin receptor LGR7 both in vitro and in vivo Biochemistry 45 2006 1043 1053
3. R.A. Bathgate, C.S. Samuel, T.C. Burazin, S. Layfield, A.A. Claasz, and I.G. Reytomas Human relaxin gene 3 (H3) and the equivalent mouse relaxin (M3) gene. Novel members of the relaxin peptide family J Biol Chem 277 2002 1148 1157
4. T.C. Burazin, R.A. Bathgate, M. Macris, S. Layfield, A.L. Gundlach, and G.W. Tregear Restricted, but abundant, expression of the novel rat gene-3 (R3) relaxin in the dorsal tegmental region of brain J Neurochem 82 2002 1553 1557
5. T. Hida, E. Takahashi, K. Shikata, T. Hirohashi, T. Sawai, and T. Seiki Chronic intracerebroventricular administration of relaxin-3 increases body weight in rats J Recept Signal Transduct Res 26 2006 147 158
6. M.A. Hossain, R.A. Bathgate, K.J. Rosengren, F. Shabanpoor, S. Zhang, and F. Lin The structural and functional role of the B-chain C-terminal arginine in the relaxin-3 peptide antagonist, R3(BDelta23-27)R/I5 Chem Biol Drug Des 73 2009 46 52
7. H. Kizawa, K. Nishi, Y. Ishibashi, M. Harada, T. Asano, and Y. Ito Production of recombinant human relaxin 3 in AtT20 cells Regul Pept 113 2003 79 84
8. C. Kuei, S. Sutton, P. Bonaventure, C. Pudiak, J. Shelton, and J. Zhu R3(BDelta23 27)R/I5 chimeric peptide, a selective antagonist for GPCR135 and GPCR142 over relaxin receptor LGR7: in vitro and in vivo characterization J Biol Chem 282 2007 25425 25435
9. C. Liu, J. Chen, C. Kuei, S. Sutton, D. Nepomuceno, and P. Bonaventure Relaxin-3/insulin-like peptide 5 chimeric peptide, a selective ligand for G protein-coupled receptor GPCR135 and GPCR142 over leucine-rich repeat-containing G protein-coupled receptor 7 Mol Pharmacol 67 2005 231 240
10. C. Liu, J. Chen, S. Sutton, B. Roland, C. Kuei, and N. Farmer Identification of relaxin-3/INSL7 as a ligand for GPCR142 J Biol Chem 278 2003 50765 50770
11. C. Liu, E. Eriste, S. Sutton, J. Chen, B. Roland, and C. Kuei Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein-coupled receptor GPCR135 J Biol Chem 278 2003 50754 50764
12. S. Ma, P. Bonaventure, T. Ferraro, P.J. Shen, T.C. Burazin, and R.A. Bathgate Relaxin-3 in GABA projection neurons of nucleus incertus suggests widespread influence on forebrain circuits via G-protein-coupled receptor-135 in the rat Neuroscience 144 2007 165 190
13. S. Ma, Q. Sang, J.L. Lanciego, and A.L. Gundlach Localization of relaxin-3 in brain of Macaca fascicularis: identification of a nucleus incertus in primate J Comp Neurol 517 2009 856 872
14. B.M. McGowan, S.A. Stanley, J. Donovan, E.L. Thompson, M. Patterson, and N.M. Semjonous Relaxin-3 stimulates the hypothalamic-pituitary-gonadal axis Am J Physiol Endocrinol Metab 295 2008 E278 E286
15. B.M. McGowan, S.A. Stanley, K.L. Smith, J.S. Minnion, J. Donovan, and E.L. Thompson Effects of acute and chronic relaxin-3 on food intake and energy expenditure in rats Regul Pept 136 2006 72 77
16. B.M. McGowan, S.A. Stanley, K.L. Smith, N.E. White, M.M. Connolly, and E.L. Thompson Central relaxin-3 administration causes hyperphagia in male Wistar rats Endocrinology 146 2005 3295 3300
17. B.M. McGowan, S.A. Stanley, N.E. White, A. Spangeus, M. Patterson, and E.L. Thompson Hypothalamic mapping of orexigenic action and Fos-like immunoreactivity following relaxin-3 administration in male Wistar rats Am J Physiol Endocrinol Metab 292 2007 E913 E919
18. K.J. Rosengren, F. Lin, R.A. Bathgate, G.W. Tregear, N.L. Daly, and J.D. Wade Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3 J Biol Chem 281 2006 5845 5851
19. F. Shabanpoor, R.A. Hughes, R.A. Bathgate, S. Zhang, D.B. Scanlon, and F. Lin Solid-phase synthesis of europium-labeled human INSL3 as a novel probe for the study of ligand-receptor interactions Bioconjug Chem 19 2008 1456 1463
20. F. Shabanpoor, F. Separovic, and J.D. Wade The human insulin superfamily of polypeptide hormones Vitam Horm 80 2009 1 31
21. J.D. Silvertown, A. Neschadim, H.N. Liu, P. Shannon, J.S. Walia, and J.C. Kao Relaxin-3 and receptors in the human and rhesus brain and reproductive tissues Regul Pept 159 2010 44 53
22. C.M. Smith, A.J. Lawrence, S.W. Sutton, and A.L. Gundlach Behavioral phenotyping of mixed background (129S5:B6) relaxin-3 knockout mice Ann N Y Acad Sci 1160 2009 236 241
23. C.M. Smith, P.J. Shen, S. Ma, S.W. Sutton, and A.L. Gundlach Verification of a relaxin-3 knockout/LacZ reporter mouse as a model of relaxin-3 deficiency Ann N Y Acad Sci 1160 2009 259 260
24. S. Sudo, J. Kumagai, S. Nishi, S. Layfield, T. Ferraro, and R.A. Bathgate H3 relaxin is a specific ligand for LGR7 and activates the receptor by interacting with both the ectodomain and the exoloop 2 J Biol Chem 278 2003 7855 7862
25. S.W. Sutton, P. Bonaventure, C. Kuei, D. Nepomuceno, J. Wu, and J. Zhu G-protein-coupled receptor (GPCR)-142 does not contribute to relaxin-3 binding in the mouse brain: further support that relaxin-3 is the physiological ligand for GPCR135 Neuroendocrinology 82 2005 139 150
26. M. Tanaka, N. Iijima, Y. Miyamoto, S. Fukusumi, Y. Itoh, and H. Ozawa Neurons expressing relaxin 3/INSL 7 in the nucleus incertus respond to stress Eur J Neurosci 21 2005 1659 1670
27. G.W. Tregear, R.A. Bathgate, S. Layfield, T. Ferraro, A. Gundlach, and S. Ma The chemistry and biology of human relaxin-3 Ann N Y Acad Sci 1041 2005 40 46