Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3

FEBS Journal

Volumn 276, Issue 18, 2009, Pages 5203-5211

  Luo, Xiao ^a   Bathgate, Ross A D ^b   Liu, Ya Li ^a   Shao, Xiao Xia ^a   Wade, John D ^b   Guo, Zhan Yun ^a  

^a TONGJI UNIVERSITY   (China)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Activity; INSL3; Recombinant expression; Refolding; Single chain precursor

Indexed keywords

CARBOXYPEPTIDASE B; CYCLIC AMP; DISULFIDE; ENDOPROTEINASE LYS C; INSULIN DERIVATIVE; INSULIN LIKE PEPTIDE 3; METAL ION; PROTEINASE; RECEPTOR; RECOMBINANT PROTEIN; RELAXIN FAMILY PEPTIDE RECEPTOR 2; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL CELL; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; IN VITRO STUDY; NONHUMAN; PEPTIDE MAPPING; PRECURSOR; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; RECEPTOR BINDING; SOLUBILIZATION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; CYCLIC AMP; HUMANS; INSULIN; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PROTEIN FOLDING; PROTEIN PRECURSORS; PROTEINS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 69449094012     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2009.07216.x     Document Type: Article

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