Overexpression of EGFR in Head and Neck Squamous Cell Carcinoma Is Associated with Inactivation of SH3GL2 and CDC25A Genes

PLoS ONE

Volumn 8, Issue 5, 2013, Pages

  Maiti, Guru Prasad ^a   Mondal, Pinaki ^b   Mukherjee, Nupur ^a   Ghosh, Amlan ^a,c   Ghosh, Susmita ^a,d   Dey, Sanjib ^b   Chakrabarty, Jayanta ^a   Roy, Anup ^e   Biswas, Jaydip ^a   Roychoudhury, Susanta ^b   Panda, Chinmay Kumar ^a  

^a CHITTARANJAN NATIONAL CANCER INSTITUTE   (India)

^b INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

^c PRESIDENCY UNIVERSITY   (India)

^d JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e NORTH BENGAL MEDICAL COLLEGE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; EPIDERMAL GROWTH FACTOR RECEPTOR; MEMBRANE PROTEIN; MESSENGER RNA; PROTEIN CDC25A; PROTEIN SH3GL2; UNCLASSIFIED DRUG;

ADULT; AMINO ACID SUBSTITUTION; ARTICLE; CANCER PROGNOSIS; CANCER STAGING; CANCER SURVIVAL; CDC25A GENE; CONTROLLED STUDY; DOWN REGULATION; EPIDERMAL GROWTH FACTOR RECEPTOR GENE; FEMALE; GENE; GENE AMPLIFICATION; GENE DELETION; GENE EXPRESSION REGULATION; GENE FREQUENCY; GENE INACTIVATION; GENE OVEREXPRESSION; GENETIC ASSOCIATION; HEAD AND NECK SQUAMOUS CELL CARCINOMA; HEP 2 CELL; HETEROZYGOSITY LOSS; HUMAN; HUMAN TISSUE; IN VITRO STUDY; MAJOR CLINICAL STUDY; MALE; MOLECULAR DYNAMICS; MUTATIONAL ANALYSIS; OUTCOME ASSESSMENT; OVERALL SURVIVAL; PAPILLOMAVIRUS INFECTION; PREDICTIVE VALUE; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN DEPHOSPHORYLATION; PROTEIN EXPRESSION; PROTEIN METHYLATION; SH3GL2 GENE; SINGLE NUCLEOTIDE POLYMORPHISM; SURVIVAL RATE; UPREGULATION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ADULT; AGED; CARCINOMA, SQUAMOUS CELL; CDC25 PHOSPHATASES; DNA METHYLATION; GENE AMPLIFICATION; GENE DELETION; GENE EXPRESSION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE SILENCING; HEAD AND NECK NEOPLASMS; HUMANS; MIDDLE AGED; MUTATION; PAPILLOMAVIRIDAE; PROMOTER REGIONS, GENETIC; RECEPTOR, EPIDERMAL GROWTH FACTOR; REPRODUCIBILITY OF RESULTS;

EID: 84877628286     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0063440     Document Type: Article

References (32)

1. Ghosh A, Ghosh S, Maiti GP, Sabbir MG, Alam N, et al. (2009) SH3GL2 and CDKN2A/2B loci are independently altered in early dysplastic lesions of head and neck: correlation with HPV infection and tobacco habit. J Pathol 217: 408-419.
2. Perez-Ordonez B, Beauchemin M, Jordan RC, (2006) Molecular biology of squamous cell carcinoma of the head and neck. J Clin Pathol 59: 445-453.
3. Chung CH, Ely K, McGavran L, Varella-Garcia M, Parker J, et al. (2006) Increased epidermal growth factor receptor gene copy number is associated with poor prognosis in head and neck squamous cell carcinomas. J Clin Oncol 24: 4170-4176.
4. Leemans CR, Braakhuis BJ, Brakenhoff RH, (2010) The molecular biology of head and neck cancer. Nat Rev Cancer 11: 9-22.
5. Chau NG, Perez-Ordonez B, Zhang K, Pham NA, Ho J, et al. (2011) The association between EGFR variant III, HPV, p16, c-MET, EGFR gene copy number and response to EGFR inhibitors in patients with recurrent or metastatic squamous cell carcinoma of the head and neck. Head Neck Oncol 3: 11.
6. Perrone F, Suardi S, Pastore E, Casieri P, Orsenigo M, et al. (2006) Molecular and cytogenetic subgroups of oropharyngeal squamous cell carcinoma. Clin Cancer Res 12: 6643-6651.
7. Choi SH, Mendrola JM, Lemmon MA, (2007) EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer. Oncogene 26: 1567-1576.
8. Xu Y, Baker D, Quan T, Baldassare JJ, Voorhees JJ, et al. (2010) Receptor type protein tyrosine phosphatase-kappa mediates cross-talk between transforming growth factor-beta and epidermal growth factor receptor signaling pathways in human keratinocytes. Mol Biol Cell 21: 29-35.
9. Dikic I, (2003) Mechanisms controlling EGF receptor endocytosis and degradation. Biochem Soc Trans 31: 1178-1181.
10. Shang C, Fu WN, Guo Y, Huang DF, Sun KL, (2007) Study of the SH3-domain GRB2-like 2 gene expression in laryngeal carcinoma. Chin Med J (Engl) 120: 385-388.
11. Wang Z, Wang M, Lazo JS, Carr BI, (2002) Identification of epidermal growth factor receptor as a target of Cdc25A protein phosphatase. J Biol Chem 277: 19470-19475.
12. Mitra S, Mazumder Indra D, Bhattacharya N, Singh RK, Basu PS, et al. (2010) RBSP3 is frequently altered in premalignant cervical lesions: clinical and prognostic significance. Genes Chromosomes Cancer 49: 155-170.
13. Dasgupta S, Mukherjee N, Roy S, Roy A, Sengupta A, et al. (2002) Mapping of the candidate tumor suppressor genes' loci on human chromosome 3 in head and neck squamous cell carcinoma of an Indian patient population. Oral Oncol 38: 6-15.
14. Ghosh S, Ghosh A, Maiti GP, Alam N, Roy A, et al. (2008) Alterations of 3p21.31 tumor suppressor genes in head and neck squamous cell carcinoma: Correlation with progression and prognosis. Int J Cancer 123: 2594-2604.
15. Bhattacharya N, Roy A, Roy B, Roychoudhury S, Panda CK, (2009) MYC gene amplification reveals clinical association with head and neck squamous cell carcinoma in Indian patients. J Oral Pathol Med 38: 759-763.
16. Tripathi A, Banerjee S, Roy A, Roychowdhury S, Panda CK, (2003) Alterations of the P16 gene in uterine cervical carcinoma from Indian patients. Int J Gynecol Cancer 13: 472-479.
17. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB, (1996) Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 93: 9821-9826.
18. Nath S, Banerjee T, Sen D, Das T, Roychoudhury S, (2011) Spindle assembly checkpoint protein Cdc20 transcriptionally activates expression of ubiquitin carrier protein UbcH10. J Biol Chem 286: 15666-15677.
19. Ghosh S, Ghosh A, Maiti GP, Alam N, Roy A, et al. (2009) Alterations of ROBO1/DUTT1 and ROBO2 loci in early dysplastic lesions of head and neck: clinical and prognostic implications. Hum Genet 125: 189-198.
20. Primer-3. Available: http://frodo.wi.mit.edu/ Accessed 2006 Aug 22.
21. TF Binding Prediction software. Available: http://www.gene-regulation.com/pub/programs/alibaba2/index.html. Accessed 2009 Jul 15.
22. EGFR at Cancer genome project database. Available: http://www.sanger.ac.uk/cgi-bin/genetics/CGP/conan/search.cgi?geneName=EGFR&action=GetGenes&searchmethod=hugoId Accessed 2012 Sep 10.
23. Sinha S, Chunder N, Mukherjee N, Alam N, Roy A, et al. (2008) Frequent deletion and methylation in SH3GL2 and CDKN2A loci are associated with early- and late-onset breast carcinoma. Ann Surg Oncol 15: 1070-1080.
24. Dasgupta S, Jang JS, Shao C, Mukhopadhyay ND, Sokhi UK, et al. (2013) SH3GL2 is frequently deleted in non-small cell lung cancer and downregulates tumor growth by modulating EGFR signaling. J Mol Med (Berl) 91: 381-393.
25. Farrell WE, Simpson DJ, Bicknell JE, Talbot AJ, Bates AS, et al. (1997) Chromosome 9p deletions in invasive and noninvasive nonfunctional pituitary adenomas: the deleted region involves markers outside of the MTS1 and MTS2 genes. Cancer Res 57: 2703-2709.
26. Giordani L, Iolascon A, Servedio V, Mazzocco K, Longo L, et al. (2002) Two regions of deletion in 9p22- p24 in neuroblastoma are frequently observed in favorable tumors. Cancer Genet Cytogenet 135: 42-47.
27. Potter N, Karakoula A, Phipps KP, Harkness W, Hayward R, et al. (2008) Genomic deletions correlate with underexpression of novel candidate genes at six loci in pediatric pilocytic astrocytoma. Neoplasia 10: 757-772.
28. SH3GL2 at Cancer genome project database. Available: http://www.sanger.ac.uk/cgi-bin/genetics/CGP/conan/search.cgi?geneName=SH3GL2&action=GetGenes&search, Accessed 2012 Sep 10.
29. Shang C, Guo Y, Fu S, Fu W, Sun K, (2010) SH3GL2 gene participates in MEK-ERK signal pathway partly by regulating EGFR in the laryngeal carcinoma cell line Hep2. Med Sci Monit 16: BR168-173.
30. CDC25A at Cancer genome project database. Available: http://www.sanger.ac.uk/cgi-bin/genetics/CGP/conan/search.cgi?geneName=CDC25A&action=GetGenes&search method = hugoId Accessed 2012 Sep 10.
31. Ray D, Kiyokawa H, (2008) CDC25A phosphatase: a rate-limiting oncogene that determines genomic stability. Cancer Res 68: 1251-1253.
32. Zhang B, Srirangam A, Potter DA, Roman A, (2005) HPV16 E5 protein disrupts the c-Cbl-EGFR interaction and EGFR ubiquitination in human foreskin keratinocytes. Oncogene 24: 2585-2588.