High-throughput single-cell analysis of low copy number β-galactosidase by a laboratory-built high-sensitivity flow cytometer

Biosensors and Bioelectronics

Volumn 48, Issue , 2013, Pages 49-55

  Yang, Lingling ^a   Huang, Tianxun ^a   Zhu, Shaobin ^a   Zhou, Yingxing ^a   Jiang, Yunbin ^a   Wang, Shuo ^a   Chen, Yuqing ^a   Wu, Lina ^a   Yan, Xiaomei ^a  

^a XIAMEN UNIVERSITY   (China)

Author keywords

galactosidase; Flow cytometry; Gene expression; Low copy number; Protein expression; Single cell analysis

Indexed keywords

COPY NUMBER; GALACTOSIDASES; HETEROGENEOUS EXPRESSIONS; HIGH-THROUGHPUT METHOD; PROTEIN EXPRESSIONS; QUANTITATIVE MEASUREMENT; SINGLECELL ANALYSIS; STATISTICAL DISTRIBUTION;

CELL CULTURE; CELL PROLIFERATION; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; TRANSCRIPTION FACTORS;

CELLS;

BETA GALACTOSIDASE;

ANALYTICAL EQUIPMENT; ARTICLE; BACTERIAL CELL; BACTERIAL COUNT; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE STAINING; FLUOROMETRY; GENE DOSAGE; HIGH SENSITIVITY FLOW CYTOMETER; HIGH THROUGHPUT SCREENING; LABORATORY; NONHUMAN; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REACTION TIME; SENSITIVITY ANALYSIS; SINGLE CELL ANALYSIS; STAINING;

BETA-GALACTOSIDASE; EQUIPMENT DESIGN; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE DOSAGE; GENE EXPRESSION; SINGLE-CELL ANALYSIS;

EID: 84877315210     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2013.03.078     Document Type: Article

References (42)

1. Ambrose W.P., Goodwin P.M., Jett J.H., Van Orden A., Werner J.H., Keller R.A. Chemical Reviews 1999, 99:2929-2956.
2. Baret J.C., Miller O.J., Taly V., Ryckelynck M., El-Harrak A., Frenz L., Rick C., Samuels M.L., Hutchison J.B., Agresti J.J., Link D.R., Weitz D.A., Griffiths A.D. Lab on a Chip 2009, 9:1850-1858.
3. Blake W.J., M K.A., Cantor C.R., Collins J.J. Nature 2003, 422:633-637.
4. Bocanegra J.A., Bejarano L.A., Valdivia M.M. Biotechniques 1997, 22(798-800):802.
5. Cai L., Friedman N., Xie X.S. Nature 2006, 440:358-362.
6. Castro A., Fairfield F.R., Shera E.B. Analytical Chemistry 1993, 65:849-852.
7. Chen D., Dovichi N.J. Analytical Chemistry 1996, 68:690-696.
8. Cookson S., Ostroff N., Pang W.L., Volfson D., Hasty J. Molecular Systems Biology 2005, 1(2005):0024.
9. Dovichi N.J., Martin J.C., Jett J.H., Keller R.A. Science 1983, 219:845-847.
10. Elowitz M.B., Levine A.J., Siggia E.D., Swain P.S. Science 2002, 297:1183-1186.
11. Gardner T.S., Cantor C.R., Collins J.J. Nature 2000, 403:339-342.
12. Ghaemmaghami S., Huh W.K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 2003, 425:737-741.
13. Guptasarma P. Bioessays 1995, 17:987-997.
14. He M., Edgar J.S., Jeffries G.D., Lorenz R.M., Shelby J.P., Chiu D.T. Analytical Chemistry 2005, 77:1539-1544.
15. Huang B., Wu H., Bhaya D., Grossman A., Granier S., Kobilka B.K., Zare R.N. Science 2007, 315:81-84.
16. Jiang T., Xing B., Rao J. Biotechnology and Genetic Engineering Reviews 2008, 25:42-76.
17. Kaern M., Elston T.C., Blake W.J., Collins J.J. Nature Reviews Genetics 2005, 6:451-464.
18. Karimova G., Dautin N., Ladant D. Journal of Bacteriology 2005, 187:2233-2243.
19. Keller R.A., Ambrose W.P., Goodwin P.M., JETr J.H., Martin J.C., Wu M. Applied Spectroscopy 1996, 50:12A-32A.
20. Kuhlman T., Zhang Z., Saier M.H., Hwa T. Proceedings of the National Academy of Sciences of the United States of America 2007, 104:6043-6048.
21. Lin Y., Trouillon R., Safina G., Ewing A.G. Analytical Chemistry 2011, 83:4369-4392.
22. Locke J.C., Young J.W., Fontes M., Hernandez Jimenez M.J., Elowitz M.B. Science 2011, 334:366-369.
23. Longo D., Hasty J. Molecular Systems Biology 2006, 2:64.
24. Miao F., Todd P., Kompala D.S. Biotechnology and Bioengineering 1993, 42:708-715.
25. Newman J.R., Ghaemmaghami S., Ihmels J., Breslow D.K., Noble M., DeRisi J.L., Weissman J.S. Nature 2006, 441:840-846.
26. Nolan G.P., Fiering S., Nicolas J.F., Herzenberg L.A. Proceedings of the National Academy of Sciences of the United States of America 1988, 85:2603-2607.
27. Ozbudak E.M., Thattai M., Kurtser I., Grossman A.D., van Oudenaarden A. Nature Genetics 2002, 31:69-73.
28. Rondelez Y., Tresset G., Tabata K.V., Arata H., Fujita H., Takeuchi S., Noji H. Nature Biotechnology 2005, 23:361-365.
29. Rosenfeld N., Young J.W., Alon U., Swain P.S., Elowitz M.B. Science 2005, 307:1962-1965.
30. Ryan D., Ren K., Wu H. Biomicrofluidics 2011, 5:21501.
31. Saida F., Uzan M., Odaert B., Bontems F. Current Protein and Peptide Science 2006, 7:47-56.
32. Schmid A., Kortmann H., Dittrich P.S., Blank L.M. Current Opinion in Biotechnology 2010, 21:12-20.
33. Taniguchi Y., Choi P.J., Li G.W., Chen H., Babu M., Hearn J., Emili A., Xie X.S. Science 2010, 329:533-538.
34. Wang D., Bodovitz S. Trends in Biotechnology 2010, 28:281-290.
35. Yang L., Wu L., Zhu S., Long Y., Hang W., Yan X. Analytical Chemistry 2010, 82:1109-1116.
36. Yang L., Zhou Y., Zhu S., Huang T., Wu L., Yan X. Analytical Chemistry 2012, 84:1526-1532.
37. Yang L., Zhu S., Hang W., Wu L., Yan X. Analytical Chemistry 2009, 81:2555-2563.
38. Zhang S., Zhu S., Yang L., Zheng Y., Gao M., Wang S., Zeng J.Z., Yan X. Analytical Chemistry 2012, 84:6421-6428.
39. Zhang Y.Z., Naleway J.J., Larison K.D., Huang Z.J., Haugland R.P. The FASEB Journal 1991, 5:3108-3113.
40. Zhao Y., He W., Liu W.F., Liu C.C., Feng L.K., Sun L., Yan Y.B., Hang H.Y. Journal of Biological Chemistry 2012, 287:9259-9268.
41. Zhu S., Yang L., Long Y., Gao M., Huang T., Hang W., Yan X. Journal of the American Chemical Society 2010, 132:12176-12178.
42. Zhu S.B., Wang S., Yang L.L., Huang T.X., Yan X.M. Science China Chemistry 2011, 54:1244-1253.