Expression of the highly toxic centromere binding protein CENP-B in E. coli using the pET system in the absence of the inducer IPTG

BioTechniques

Volumn 22, Issue 5, 1997, Pages 798-802

  Bocanegra, J Antonio ^a   Bejarano, Luis A ^a   Valdivia, Manuel M ^a  

^a UNIVERSITY OF CÁDIZ   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GROWTH; CELL GROWTH; CENTROMERE; ESCHERICHIA COLI; NONHUMAN; POSITRON EMISSION TOMOGRAPHY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030908148     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97225bm02     Document Type: Article

References (12)

1. Bejarano, L.A. and M.M. Valdivia. 1996. Molecular cloning of an intronless gene for the hamster centromere antigen CENP-B. Biochem. Biophys. Acta 1307:21-25.
2. Brosius, J. 1984. Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators. Gene 27:161-172.
3. Earnshaw, W.C., K.F. Sullivan, P.S. Machlin, C.A. Cooke, D.A. Kaiser, T.D. Pollard, N.F. Rothfield and D.W. Cleveland. 1987 Molecular cloning of a cDNA for CENP-B the major human centromere autoantigen. J. Cell Biol. 104:817-829.
4. Matsumoto, H., K. Sugimoto and T. Okazaki. 1989. A human centromere antigen (CENP-B) interacts with a short sequence in alphoid DNA, a human centromeric satellite. J. Cell Biol. 109:1963-1973.
5. Moroi, Y., C. Peebles, M.J. Fritzler, J. Steigerwald and E.M. Tan. 1980. Autoantibody to centromere (kinetochore) in scleroderma sera. Proc. Natl. Acad. Sci. USA 77:1627-1631.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Sisk, W.P., J.D. Bradley, D. Kingsley and T.A. Patterson. 1992. Deletion of Hydrophobic domains of viral glycoproteins increases the level of their production in Escherichia coli. Gene 112:157-162.
8. Studier, F.W. and B.A. Mofatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:60-89.
9. Sugimoto, K., Y. Hagishita and M. Himeno. 1994. Functional domain structure of human centromere protein B. Implication of the internal and C-terminal self-association domains in centromeric heterochromatin condensation. J. Biol. Chem. 269:24271-24276.
10. Sullivan, K.F. and C.A. Glass. 1991. CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins. Chromosoma 100:360-370.
11. Suter-Crazzolara, C. and K. Unsicker. 1995. Improved expression of toxic proteins in E. coli. BioTechniques 19:202-204.
12. Verheijen, R., B.A.W. de Jong, E.H.H. Oberjé, and W.J. van Venrooij. 1992. Molecular cloning of a major CENP-B epitope and its use for the detection of anticentromere autoantibodies. Mol. Biol. Rep. 16:49-59.